TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envexpbot.2022.104908 http://hdl.handle.net/11449/241320 |
Resumo: | Stomatal conductance (gs) takes longer to recover after drought when compared to leaf water potential or leaf water content (RWC). Aquaporins, known for transporting water through membranes, could modulate gs recovery. Using Sorghum bicolor, we tested whether members of the plasma membrane (PIP) and tonoplast (TIP) intrinsic aquaporin subfamilies could influence gs after a 20-day drought. Potted plants under soil field capacity (control), severe water deficit (SWD; 30% (m/m) soil water content), and progressive severe water deficit (PSWD; water withheld until maximum stress) were used. Soil (Ψsoil), predawn (ΨPD), and midday (ΨMD) leaf water potentials, photochemical, and gas exchange parameters were measured during drought and 12 days of recovery. After 17 days of drought, gs of SWD and PSWD was 4-times lower than control, and it took 6 days to recover. ΨPD, ΨMD, and RWC, however, recovered after 24 h. SbPIP1;2, SbPIP2;5, and SbTIP1;1 were all down-regulated on the day of maximum stress, but only SbTIP1;1 showed consistent alleviation of its down-regulation as gs recovered. SbTIP1;1 expression was positively correlated with gs, leaf transpiration, and hydraulic conductivity (KL), but only in SWD plants. The slow gs recovery seems to be associated with the alleviation of SbTIP1;1 down-regulation as rehydration progresses. |
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TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolorAquaporinsDrought recoverygsPIPReirrigationTIPStomatal conductance (gs) takes longer to recover after drought when compared to leaf water potential or leaf water content (RWC). Aquaporins, known for transporting water through membranes, could modulate gs recovery. Using Sorghum bicolor, we tested whether members of the plasma membrane (PIP) and tonoplast (TIP) intrinsic aquaporin subfamilies could influence gs after a 20-day drought. Potted plants under soil field capacity (control), severe water deficit (SWD; 30% (m/m) soil water content), and progressive severe water deficit (PSWD; water withheld until maximum stress) were used. Soil (Ψsoil), predawn (ΨPD), and midday (ΨMD) leaf water potentials, photochemical, and gas exchange parameters were measured during drought and 12 days of recovery. After 17 days of drought, gs of SWD and PSWD was 4-times lower than control, and it took 6 days to recover. ΨPD, ΨMD, and RWC, however, recovered after 24 h. SbPIP1;2, SbPIP2;5, and SbTIP1;1 were all down-regulated on the day of maximum stress, but only SbTIP1;1 showed consistent alleviation of its down-regulation as gs recovered. SbTIP1;1 expression was positively correlated with gs, leaf transpiration, and hydraulic conductivity (KL), but only in SWD plants. The slow gs recovery seems to be associated with the alleviation of SbTIP1;1 down-regulation as rehydration progresses.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Bioestatística Biologia vegetal Parasitologia e Zoologia Instituto de Biociências Universidade Estadual Paulista UNESP, São PauloDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista UNESP, São PauloDepartamento de Biodiversidade Universidade Estadual Paulista UNESP Instituto de Biociências, Av. 24-A, 1515, SPDepartamento de Bioestatística Biologia vegetal Parasitologia e Zoologia Instituto de Biociências Universidade Estadual Paulista UNESP, São PauloDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista UNESP, São PauloDepartamento de Biodiversidade Universidade Estadual Paulista UNESP Instituto de Biociências, Av. 24-A, 1515, SPFAPESP: #2010/15585-6CNPq: #307431/2020-7Universidade Estadual Paulista (UNESP)Schley, Thayssa Rabelo [UNESP]Franco, Danilo Miralha [UNESP]Araújo Junior, João Pessoa [UNESP]de Godoy Maia, Ivan [UNESP]Habermann, Gustavo [UNESP]de Almeida, Luiz Fernando Rolim [UNESP]2023-03-01T20:56:46Z2023-03-01T20:56:46Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.envexpbot.2022.104908Environmental and Experimental Botany, v. 200.0098-8472http://hdl.handle.net/11449/24132010.1016/j.envexpbot.2022.1049082-s2.0-85133953098Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental and Experimental Botanyinfo:eu-repo/semantics/openAccess2023-03-01T20:56:46Zoai:repositorio.unesp.br:11449/241320Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:50:37.563482Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
title |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
spellingShingle |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor Schley, Thayssa Rabelo [UNESP] Aquaporins Drought recovery gs PIP Reirrigation TIP |
title_short |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
title_full |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
title_fullStr |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
title_full_unstemmed |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
title_sort |
TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor |
author |
Schley, Thayssa Rabelo [UNESP] |
author_facet |
Schley, Thayssa Rabelo [UNESP] Franco, Danilo Miralha [UNESP] Araújo Junior, João Pessoa [UNESP] de Godoy Maia, Ivan [UNESP] Habermann, Gustavo [UNESP] de Almeida, Luiz Fernando Rolim [UNESP] |
author_role |
author |
author2 |
Franco, Danilo Miralha [UNESP] Araújo Junior, João Pessoa [UNESP] de Godoy Maia, Ivan [UNESP] Habermann, Gustavo [UNESP] de Almeida, Luiz Fernando Rolim [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Schley, Thayssa Rabelo [UNESP] Franco, Danilo Miralha [UNESP] Araújo Junior, João Pessoa [UNESP] de Godoy Maia, Ivan [UNESP] Habermann, Gustavo [UNESP] de Almeida, Luiz Fernando Rolim [UNESP] |
dc.subject.por.fl_str_mv |
Aquaporins Drought recovery gs PIP Reirrigation TIP |
topic |
Aquaporins Drought recovery gs PIP Reirrigation TIP |
description |
Stomatal conductance (gs) takes longer to recover after drought when compared to leaf water potential or leaf water content (RWC). Aquaporins, known for transporting water through membranes, could modulate gs recovery. Using Sorghum bicolor, we tested whether members of the plasma membrane (PIP) and tonoplast (TIP) intrinsic aquaporin subfamilies could influence gs after a 20-day drought. Potted plants under soil field capacity (control), severe water deficit (SWD; 30% (m/m) soil water content), and progressive severe water deficit (PSWD; water withheld until maximum stress) were used. Soil (Ψsoil), predawn (ΨPD), and midday (ΨMD) leaf water potentials, photochemical, and gas exchange parameters were measured during drought and 12 days of recovery. After 17 days of drought, gs of SWD and PSWD was 4-times lower than control, and it took 6 days to recover. ΨPD, ΨMD, and RWC, however, recovered after 24 h. SbPIP1;2, SbPIP2;5, and SbTIP1;1 were all down-regulated on the day of maximum stress, but only SbTIP1;1 showed consistent alleviation of its down-regulation as gs recovered. SbTIP1;1 expression was positively correlated with gs, leaf transpiration, and hydraulic conductivity (KL), but only in SWD plants. The slow gs recovery seems to be associated with the alleviation of SbTIP1;1 down-regulation as rehydration progresses. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:56:46Z 2023-03-01T20:56:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envexpbot.2022.104908 Environmental and Experimental Botany, v. 200. 0098-8472 http://hdl.handle.net/11449/241320 10.1016/j.envexpbot.2022.104908 2-s2.0-85133953098 |
url |
http://dx.doi.org/10.1016/j.envexpbot.2022.104908 http://hdl.handle.net/11449/241320 |
identifier_str_mv |
Environmental and Experimental Botany, v. 200. 0098-8472 10.1016/j.envexpbot.2022.104908 2-s2.0-85133953098 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental and Experimental Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129365259583488 |