Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors

Detalhes bibliográficos
Autor(a) principal: Bertanha, Matheus [UNESP]
Data de Publicação: 2013
Outros Autores: Moroz, Andrei[UNESP], Almeida, Rodrigo, Alves, Flavia Cilene, Acorci Valério, Michele Janegitz, Moura, Regina [UNESP], Domingues, Maria Aparecida Custódio [UNESP], Sobreira, Marcone Lima [UNESP], Deffune, Elenice [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jvs.2013.05.032
http://hdl.handle.net/11449/75907
Resumo: Background: Cardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration of the circulation through construction of bypass surgical treatment is regarded as the gold standard treatment of peripheral vascular diseases, and grafts are necessary for this purpose. The great saphenous vein is often not available and synthetic grafts have their limitations. Therefore, new techniques to produce alternative grafts have been developed and, in this sense, tissue engineering is a promising alternative to provide biocompatible grafts. This study objective was to reconstruct the endothelium layer of decellularized vein scaffolds, using mesenchymal stem cells (MSCs) and growth factors obtained from platelets. Methods: Fifteen nonpregnant female adult rabbits were used for all experiments. Adipose tissue and vena cava were obtained and subjected to MSCs isolation and tissue decellularization, respectively. MSCs were subjected to differentiation using endothelial inductor growth factor (EIGF) obtained from human platelet lysates. Immunofluorescence, histological and immunohistochemical analyses were employed for the final characterization of the obtained blood vessel substitute. Results: The scaffolds were successfully decellularized with sodium dodecyl sulfate. MSCs actively adhered at the scaffolds, and through stimulation with EIGF were differentiated into functional endothelial cells, secreting significantly higher quantities of von Willebrand factor (0.85 μg/mL; P < .05) than cells cultivated under the same conditions, without EIGF (0.085 μg/mL). Cells with evident morphologic characteristics of endothelium were seen at the lumen of the scaffolds. These cells also stained positive for fascin protein, which is highly expressed by differentiated endothelial cells. Conclusions: Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation. © 2013 Society for Vascular Surgery.
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spelling Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factorsBackground: Cardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration of the circulation through construction of bypass surgical treatment is regarded as the gold standard treatment of peripheral vascular diseases, and grafts are necessary for this purpose. The great saphenous vein is often not available and synthetic grafts have their limitations. Therefore, new techniques to produce alternative grafts have been developed and, in this sense, tissue engineering is a promising alternative to provide biocompatible grafts. This study objective was to reconstruct the endothelium layer of decellularized vein scaffolds, using mesenchymal stem cells (MSCs) and growth factors obtained from platelets. Methods: Fifteen nonpregnant female adult rabbits were used for all experiments. Adipose tissue and vena cava were obtained and subjected to MSCs isolation and tissue decellularization, respectively. MSCs were subjected to differentiation using endothelial inductor growth factor (EIGF) obtained from human platelet lysates. Immunofluorescence, histological and immunohistochemical analyses were employed for the final characterization of the obtained blood vessel substitute. Results: The scaffolds were successfully decellularized with sodium dodecyl sulfate. MSCs actively adhered at the scaffolds, and through stimulation with EIGF were differentiated into functional endothelial cells, secreting significantly higher quantities of von Willebrand factor (0.85 μg/mL; P < .05) than cells cultivated under the same conditions, without EIGF (0.085 μg/mL). Cells with evident morphologic characteristics of endothelium were seen at the lumen of the scaffolds. These cells also stained positive for fascin protein, which is highly expressed by differentiated endothelial cells. Conclusions: Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation. © 2013 Society for Vascular Surgery.Universidade Estadual Paulista (Unesp)Bertanha, Matheus [UNESP]Moroz, Andrei[UNESP]Almeida, RodrigoAlves, Flavia CileneAcorci Valério, Michele JanegitzMoura, Regina [UNESP]Domingues, Maria Aparecida Custódio [UNESP]Sobreira, Marcone Lima [UNESP]Deffune, Elenice [UNESP]2014-05-27T11:29:55Z2014-05-27T11:29:55Z2013-07-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.jvs.2013.05.032Journal of Vascular Surgery.0741-52141097-6809http://hdl.handle.net/11449/7590710.1016/j.jvs.2013.05.032WOS:0003363638000312-s2.0-848794839632-s2.0-84879483963.pdf9646764071339214960932483259138205857231130371404513014379461383Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Vascular Surgery3.2942,3082,308info:eu-repo/semantics/openAccess2024-09-03T14:29:56Zoai:repositorio.unesp.br:11449/75907Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
title Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
spellingShingle Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
Bertanha, Matheus [UNESP]
title_short Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
title_full Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
title_fullStr Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
title_full_unstemmed Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
title_sort Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors
author Bertanha, Matheus [UNESP]
author_facet Bertanha, Matheus [UNESP]
Moroz, Andrei[UNESP]
Almeida, Rodrigo
Alves, Flavia Cilene
Acorci Valério, Michele Janegitz
Moura, Regina [UNESP]
Domingues, Maria Aparecida Custódio [UNESP]
Sobreira, Marcone Lima [UNESP]
Deffune, Elenice [UNESP]
author_role author
author2 Moroz, Andrei[UNESP]
Almeida, Rodrigo
Alves, Flavia Cilene
Acorci Valério, Michele Janegitz
Moura, Regina [UNESP]
Domingues, Maria Aparecida Custódio [UNESP]
Sobreira, Marcone Lima [UNESP]
Deffune, Elenice [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Bertanha, Matheus [UNESP]
Moroz, Andrei[UNESP]
Almeida, Rodrigo
Alves, Flavia Cilene
Acorci Valério, Michele Janegitz
Moura, Regina [UNESP]
Domingues, Maria Aparecida Custódio [UNESP]
Sobreira, Marcone Lima [UNESP]
Deffune, Elenice [UNESP]
description Background: Cardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration of the circulation through construction of bypass surgical treatment is regarded as the gold standard treatment of peripheral vascular diseases, and grafts are necessary for this purpose. The great saphenous vein is often not available and synthetic grafts have their limitations. Therefore, new techniques to produce alternative grafts have been developed and, in this sense, tissue engineering is a promising alternative to provide biocompatible grafts. This study objective was to reconstruct the endothelium layer of decellularized vein scaffolds, using mesenchymal stem cells (MSCs) and growth factors obtained from platelets. Methods: Fifteen nonpregnant female adult rabbits were used for all experiments. Adipose tissue and vena cava were obtained and subjected to MSCs isolation and tissue decellularization, respectively. MSCs were subjected to differentiation using endothelial inductor growth factor (EIGF) obtained from human platelet lysates. Immunofluorescence, histological and immunohistochemical analyses were employed for the final characterization of the obtained blood vessel substitute. Results: The scaffolds were successfully decellularized with sodium dodecyl sulfate. MSCs actively adhered at the scaffolds, and through stimulation with EIGF were differentiated into functional endothelial cells, secreting significantly higher quantities of von Willebrand factor (0.85 μg/mL; P < .05) than cells cultivated under the same conditions, without EIGF (0.085 μg/mL). Cells with evident morphologic characteristics of endothelium were seen at the lumen of the scaffolds. These cells also stained positive for fascin protein, which is highly expressed by differentiated endothelial cells. Conclusions: Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation. © 2013 Society for Vascular Surgery.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-03
2014-05-27T11:29:55Z
2014-05-27T11:29:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jvs.2013.05.032
Journal of Vascular Surgery.
0741-5214
1097-6809
http://hdl.handle.net/11449/75907
10.1016/j.jvs.2013.05.032
WOS:000336363800031
2-s2.0-84879483963
2-s2.0-84879483963.pdf
9646764071339214
9609324832591382
0585723113037140
4513014379461383
url http://dx.doi.org/10.1016/j.jvs.2013.05.032
http://hdl.handle.net/11449/75907
identifier_str_mv Journal of Vascular Surgery.
0741-5214
1097-6809
10.1016/j.jvs.2013.05.032
WOS:000336363800031
2-s2.0-84879483963
2-s2.0-84879483963.pdf
9646764071339214
9609324832591382
0585723113037140
4513014379461383
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Vascular Surgery
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reponame:Repositório Institucional da UNESP
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instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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