Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1167/iovs.09-4029 http://repositorio.unifesp.br/handle/11600/32300 |
Resumo: | PURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029 |
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Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem CellsPURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap, Inst Butantan, Genet Lab, BR-05503900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, CASO, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, Setor Cornea & Doencas Externas, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilAtividades Vet Ltda, Ctr Inovacao Tecnol, Genet Aplicada, São Paulo, BrazilClin Pesquisa Odontol CERA, São Paulo, BrazilCtr Pesquisa Odontol CERA, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, CASO, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, Setor Cornea & Doencas Externas, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Clinica CERAAssoc Research Vision Ophthalmology IncInst Nacl Ciencia & Tecnol Celulas Tronco & TerapUniversidade Federal de São Paulo (UNIFESP)Atividades Vet LtdaClin Pesquisa Odontol CERACtr Pesquisa Odontol CERAGomes, José Álvaro Pereira [UNIFESP]Monteiro, Babyla GeraldesMelo, Gustavo Barreto [UNIFESP]Smith, Ricardo Luiz [UNIFESP]Silva, Marcelo Cavenaghi Pereira da [UNIFESP]Lizier, Nelson Foresto [UNIFESP]Kerkis, AlexandreCerruti, HumbertoKerkis, Irina2016-01-24T13:59:22Z2016-01-24T13:59:22Z2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1408-1414http://dx.doi.org/10.1167/iovs.09-4029Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010.10.1167/iovs.09-40290146-0404http://repositorio.unifesp.br/handle/11600/32300WOS:000275164300025engInvestigative Ophthalmology & Visual Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-26T09:52:42Zoai:repositorio.unifesp.br/:11600/32300Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-26T09:52:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
title |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
spellingShingle |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells Gomes, José Álvaro Pereira [UNIFESP] |
title_short |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
title_full |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
title_fullStr |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
title_full_unstemmed |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
title_sort |
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells |
author |
Gomes, José Álvaro Pereira [UNIFESP] |
author_facet |
Gomes, José Álvaro Pereira [UNIFESP] Monteiro, Babyla Geraldes Melo, Gustavo Barreto [UNIFESP] Smith, Ricardo Luiz [UNIFESP] Silva, Marcelo Cavenaghi Pereira da [UNIFESP] Lizier, Nelson Foresto [UNIFESP] Kerkis, Alexandre Cerruti, Humberto Kerkis, Irina |
author_role |
author |
author2 |
Monteiro, Babyla Geraldes Melo, Gustavo Barreto [UNIFESP] Smith, Ricardo Luiz [UNIFESP] Silva, Marcelo Cavenaghi Pereira da [UNIFESP] Lizier, Nelson Foresto [UNIFESP] Kerkis, Alexandre Cerruti, Humberto Kerkis, Irina |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap Universidade Federal de São Paulo (UNIFESP) Atividades Vet Ltda Clin Pesquisa Odontol CERA Ctr Pesquisa Odontol CERA |
dc.contributor.author.fl_str_mv |
Gomes, José Álvaro Pereira [UNIFESP] Monteiro, Babyla Geraldes Melo, Gustavo Barreto [UNIFESP] Smith, Ricardo Luiz [UNIFESP] Silva, Marcelo Cavenaghi Pereira da [UNIFESP] Lizier, Nelson Foresto [UNIFESP] Kerkis, Alexandre Cerruti, Humberto Kerkis, Irina |
description |
PURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029 |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 2016-01-24T13:59:22Z 2016-01-24T13:59:22Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1167/iovs.09-4029 Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010. 10.1167/iovs.09-4029 0146-0404 http://repositorio.unifesp.br/handle/11600/32300 WOS:000275164300025 |
url |
http://dx.doi.org/10.1167/iovs.09-4029 http://repositorio.unifesp.br/handle/11600/32300 |
identifier_str_mv |
Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010. 10.1167/iovs.09-4029 0146-0404 WOS:000275164300025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Investigative Ophthalmology & Visual Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1408-1414 |
dc.publisher.none.fl_str_mv |
Assoc Research Vision Ophthalmology Inc |
publisher.none.fl_str_mv |
Assoc Research Vision Ophthalmology Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268355643703296 |