Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells

Detalhes bibliográficos
Autor(a) principal: Gomes, José Álvaro Pereira [UNIFESP]
Data de Publicação: 2010
Outros Autores: Monteiro, Babyla Geraldes, Melo, Gustavo Barreto [UNIFESP], Smith, Ricardo Luiz [UNIFESP], Silva, Marcelo Cavenaghi Pereira da [UNIFESP], Lizier, Nelson Foresto [UNIFESP], Kerkis, Alexandre, Cerruti, Humberto, Kerkis, Irina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1167/iovs.09-4029
http://repositorio.unifesp.br/handle/11600/32300
Resumo: PURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029
id UFSP_d25829aad1e52cfad6187f83a3423fe2
oai_identifier_str oai:repositorio.unifesp.br/:11600/32300
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem CellsPURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap, Inst Butantan, Genet Lab, BR-05503900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, CASO, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, Setor Cornea & Doencas Externas, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilAtividades Vet Ltda, Ctr Inovacao Tecnol, Genet Aplicada, São Paulo, BrazilClin Pesquisa Odontol CERA, São Paulo, BrazilCtr Pesquisa Odontol CERA, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, CASO, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Visao, Setor Cornea & Doencas Externas, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Clinica CERAAssoc Research Vision Ophthalmology IncInst Nacl Ciencia & Tecnol Celulas Tronco & TerapUniversidade Federal de São Paulo (UNIFESP)Atividades Vet LtdaClin Pesquisa Odontol CERACtr Pesquisa Odontol CERAGomes, José Álvaro Pereira [UNIFESP]Monteiro, Babyla GeraldesMelo, Gustavo Barreto [UNIFESP]Smith, Ricardo Luiz [UNIFESP]Silva, Marcelo Cavenaghi Pereira da [UNIFESP]Lizier, Nelson Foresto [UNIFESP]Kerkis, AlexandreCerruti, HumbertoKerkis, Irina2016-01-24T13:59:22Z2016-01-24T13:59:22Z2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1408-1414http://dx.doi.org/10.1167/iovs.09-4029Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010.10.1167/iovs.09-40290146-0404http://repositorio.unifesp.br/handle/11600/32300WOS:000275164300025engInvestigative Ophthalmology & Visual Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-26T09:52:42Zoai:repositorio.unifesp.br/:11600/32300Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-26T09:52:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
title Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
spellingShingle Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
Gomes, José Álvaro Pereira [UNIFESP]
title_short Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
title_full Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
title_fullStr Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
title_full_unstemmed Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
title_sort Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells
author Gomes, José Álvaro Pereira [UNIFESP]
author_facet Gomes, José Álvaro Pereira [UNIFESP]
Monteiro, Babyla Geraldes
Melo, Gustavo Barreto [UNIFESP]
Smith, Ricardo Luiz [UNIFESP]
Silva, Marcelo Cavenaghi Pereira da [UNIFESP]
Lizier, Nelson Foresto [UNIFESP]
Kerkis, Alexandre
Cerruti, Humberto
Kerkis, Irina
author_role author
author2 Monteiro, Babyla Geraldes
Melo, Gustavo Barreto [UNIFESP]
Smith, Ricardo Luiz [UNIFESP]
Silva, Marcelo Cavenaghi Pereira da [UNIFESP]
Lizier, Nelson Foresto [UNIFESP]
Kerkis, Alexandre
Cerruti, Humberto
Kerkis, Irina
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Inst Nacl Ciencia & Tecnol Celulas Tronco & Terap
Universidade Federal de São Paulo (UNIFESP)
Atividades Vet Ltda
Clin Pesquisa Odontol CERA
Ctr Pesquisa Odontol CERA
dc.contributor.author.fl_str_mv Gomes, José Álvaro Pereira [UNIFESP]
Monteiro, Babyla Geraldes
Melo, Gustavo Barreto [UNIFESP]
Smith, Ricardo Luiz [UNIFESP]
Silva, Marcelo Cavenaghi Pereira da [UNIFESP]
Lizier, Nelson Foresto [UNIFESP]
Kerkis, Alexandre
Cerruti, Humberto
Kerkis, Irina
description PURPOSE. To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD).METHODS. LSCD was induced by the application of 0.5 M NaOH to the right eye of rabbits for 25 seconds (mild chemical burn [MCB]) and for 45 seconds (severe chemical burn [SCB]). After 1 month, a superficial keratectomy was performed to remove the fibrovascular pannus that covered the animals' burned corneas. A tissue-engineered hIDPSC sheet was transplanted onto the corneal bed and then covered with deepithelialized human amniotic membrane (AM). in the respective control groups, the denuded cornea was covered with AM only. After 3 months, a detailed analysis of the rabbit eyes was performed with regard to clinical aspect, histology, electron microscopy, and immunohistochemistry.RESULTS. Corneal transparency of the rabbit eyes that underwent hIDPSC transplantation was improved throughout the follow-up, while the control corneas developed total conjunctivalization and opacification. Rabbits from the MCB group showed clearer corneas with less neovascularization. the clinical data were confirmed by histologic analysis that showed healthy uniform corneal epithelium, especially in the MCB group. the presence of hIDPSC was detected using an anti-hIDPSC antibody. the corneal tissue also showed positive immunostaining with anti-human antibodies. in the control corneas, none of these antigens were detected.CONCLUSIONS. Overall, these data showed that transplantation of a tissue-engineered hIDPSC sheet was successful for the reconstruction of corneal epithelium in an animal model of LSCD. (Invest Ophthalmol Vis Sci. 2010;51:1408-1414) DOI:10.1167/iovs.09-4029
publishDate 2010
dc.date.none.fl_str_mv 2010-03-01
2016-01-24T13:59:22Z
2016-01-24T13:59:22Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1167/iovs.09-4029
Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010.
10.1167/iovs.09-4029
0146-0404
http://repositorio.unifesp.br/handle/11600/32300
WOS:000275164300025
url http://dx.doi.org/10.1167/iovs.09-4029
http://repositorio.unifesp.br/handle/11600/32300
identifier_str_mv Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 51, n. 3, p. 1408-1414, 2010.
10.1167/iovs.09-4029
0146-0404
WOS:000275164300025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Investigative Ophthalmology & Visual Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1408-1414
dc.publisher.none.fl_str_mv Assoc Research Vision Ophthalmology Inc
publisher.none.fl_str_mv Assoc Research Vision Ophthalmology Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268355643703296