Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1477-7827-9-100 http://hdl.handle.net/11449/12262 |
Resumo: | Background: Hyperglycemia can impair the male reproductive system in experimental animals and in men during reproductive age. Studies have shown that vitamin C has some good effects on male reproductive system, and therefore vitamin C treatment could attenuate the dysfunctions in this system caused by hyperglycemia. Thus, the objective of this work was to evaluate whether vitamin C treatment could attenuate reproductive dysfunctions in hyperglycemic male rats.Methods: Adult male rats were divided into 3 groups: a normoglycemic (n = 10) and two hyperglycemic (that received a single dose of streptozotocin -40 mg/kg BW). The two last groups (n = 10 per group) were divided into: hyperglycemic control (Hy) and hyperglycemic + 150 mg of vitamin C (HyC), by gavage during 30 consecutive days. The normoglycemic and hyperglycemic control groups received the vehicle (water). The first day after the treatment, the rats were anesthetized and killed to evaluate oxidative stress biomarkers (TBARS, SOD, GSHt and GSH-Px) in the erythrocytes, body and reproductive organ weights, sperm parameters, plasma hormone levels (FSH, LH and testosterone), testicular and epididymal histo-morphometry and histopathology.Results: Compared with the normoglycemic animals, hyperglycemic control rats showed reduced weight of the body and reproductive organ but testis weight was maintained. It was also observed reduction of testosterone and LH levels, seminiferous tubular diameter, sperm motility and sperm counts in the epididymis. In addition, there was an increase in morphological abnormalities on spermatozoa as well as in oxidative stress level. Vitamin C reduced the oxidative stress level, diminished the number of abnormal sperm, and increased testosterone and LH levels and seminiferous tubular diameter but did not show improvement of sperm motility in relation to the hyperglycemic control group. Hyperglycemia caused a rearrangement in the epididymal tissue components (stroma, ephitelium and lumen) as demonstrated by the stereological analysis results. However, this alteration was partially prevented by vitamin C treatment.Conclusions: We conclude that vitamin C partially attenuated some male reproductive system dysfunctions in hyperglycemic rats. |
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Vitamin C partially attenuates male reproductive deficits in hyperglycemic ratsBackground: Hyperglycemia can impair the male reproductive system in experimental animals and in men during reproductive age. Studies have shown that vitamin C has some good effects on male reproductive system, and therefore vitamin C treatment could attenuate the dysfunctions in this system caused by hyperglycemia. Thus, the objective of this work was to evaluate whether vitamin C treatment could attenuate reproductive dysfunctions in hyperglycemic male rats.Methods: Adult male rats were divided into 3 groups: a normoglycemic (n = 10) and two hyperglycemic (that received a single dose of streptozotocin -40 mg/kg BW). The two last groups (n = 10 per group) were divided into: hyperglycemic control (Hy) and hyperglycemic + 150 mg of vitamin C (HyC), by gavage during 30 consecutive days. The normoglycemic and hyperglycemic control groups received the vehicle (water). The first day after the treatment, the rats were anesthetized and killed to evaluate oxidative stress biomarkers (TBARS, SOD, GSHt and GSH-Px) in the erythrocytes, body and reproductive organ weights, sperm parameters, plasma hormone levels (FSH, LH and testosterone), testicular and epididymal histo-morphometry and histopathology.Results: Compared with the normoglycemic animals, hyperglycemic control rats showed reduced weight of the body and reproductive organ but testis weight was maintained. It was also observed reduction of testosterone and LH levels, seminiferous tubular diameter, sperm motility and sperm counts in the epididymis. In addition, there was an increase in morphological abnormalities on spermatozoa as well as in oxidative stress level. Vitamin C reduced the oxidative stress level, diminished the number of abnormal sperm, and increased testosterone and LH levels and seminiferous tubular diameter but did not show improvement of sperm motility in relation to the hyperglycemic control group. Hyperglycemia caused a rearrangement in the epididymal tissue components (stroma, ephitelium and lumen) as demonstrated by the stereological analysis results. However, this alteration was partially prevented by vitamin C treatment.Conclusions: We conclude that vitamin C partially attenuated some male reproductive system dysfunctions in hyperglycemic rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Campinas UNICAMP, Inst Biol, Grad Program Cell & Struct Biol, Campinas, SP, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, BrazilUSP, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP, BrazilUNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilBiomed Central Ltd.Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Fernandes, Glaura S. A. [UNESP]Fernandez, Carla D. B. [UNESP]Campos, Kleber E. [UNESP]Damasceno, Débora Cristina [UNESP]Anselmo-Franci, Janete A.Kempinas, Wilma D. G. [UNESP]2014-05-20T13:35:36Z2014-05-20T13:35:36Z2011-07-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1186/1477-7827-9-100Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011.1477-7827http://hdl.handle.net/11449/1226210.1186/1477-7827-9-100WOS:000294539000001WOS000294539000001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Biology and Endocrinology2.8521,203info:eu-repo/semantics/openAccess2024-08-16T14:12:50Zoai:repositorio.unesp.br:11449/12262Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:12:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
title |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
spellingShingle |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats Fernandes, Glaura S. A. [UNESP] |
title_short |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
title_full |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
title_fullStr |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
title_full_unstemmed |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
title_sort |
Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats |
author |
Fernandes, Glaura S. A. [UNESP] |
author_facet |
Fernandes, Glaura S. A. [UNESP] Fernandez, Carla D. B. [UNESP] Campos, Kleber E. [UNESP] Damasceno, Débora Cristina [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
author_role |
author |
author2 |
Fernandez, Carla D. B. [UNESP] Campos, Kleber E. [UNESP] Damasceno, Débora Cristina [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Fernandes, Glaura S. A. [UNESP] Fernandez, Carla D. B. [UNESP] Campos, Kleber E. [UNESP] Damasceno, Débora Cristina [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
description |
Background: Hyperglycemia can impair the male reproductive system in experimental animals and in men during reproductive age. Studies have shown that vitamin C has some good effects on male reproductive system, and therefore vitamin C treatment could attenuate the dysfunctions in this system caused by hyperglycemia. Thus, the objective of this work was to evaluate whether vitamin C treatment could attenuate reproductive dysfunctions in hyperglycemic male rats.Methods: Adult male rats were divided into 3 groups: a normoglycemic (n = 10) and two hyperglycemic (that received a single dose of streptozotocin -40 mg/kg BW). The two last groups (n = 10 per group) were divided into: hyperglycemic control (Hy) and hyperglycemic + 150 mg of vitamin C (HyC), by gavage during 30 consecutive days. The normoglycemic and hyperglycemic control groups received the vehicle (water). The first day after the treatment, the rats were anesthetized and killed to evaluate oxidative stress biomarkers (TBARS, SOD, GSHt and GSH-Px) in the erythrocytes, body and reproductive organ weights, sperm parameters, plasma hormone levels (FSH, LH and testosterone), testicular and epididymal histo-morphometry and histopathology.Results: Compared with the normoglycemic animals, hyperglycemic control rats showed reduced weight of the body and reproductive organ but testis weight was maintained. It was also observed reduction of testosterone and LH levels, seminiferous tubular diameter, sperm motility and sperm counts in the epididymis. In addition, there was an increase in morphological abnormalities on spermatozoa as well as in oxidative stress level. Vitamin C reduced the oxidative stress level, diminished the number of abnormal sperm, and increased testosterone and LH levels and seminiferous tubular diameter but did not show improvement of sperm motility in relation to the hyperglycemic control group. Hyperglycemia caused a rearrangement in the epididymal tissue components (stroma, ephitelium and lumen) as demonstrated by the stereological analysis results. However, this alteration was partially prevented by vitamin C treatment.Conclusions: We conclude that vitamin C partially attenuated some male reproductive system dysfunctions in hyperglycemic rats. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-27 2014-05-20T13:35:36Z 2014-05-20T13:35:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1477-7827-9-100 Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011. 1477-7827 http://hdl.handle.net/11449/12262 10.1186/1477-7827-9-100 WOS:000294539000001 WOS000294539000001.pdf |
url |
http://dx.doi.org/10.1186/1477-7827-9-100 http://hdl.handle.net/11449/12262 |
identifier_str_mv |
Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011. 1477-7827 10.1186/1477-7827-9-100 WOS:000294539000001 WOS000294539000001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Biology and Endocrinology 2.852 1,203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128190575542272 |