Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1007/s12686-016-0607-y http://hdl.handle.net/11449/174365 |
Resumo: | The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs. |
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Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samplesAmelogeninSexingSRYTapirZFX/ZFYThe knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 ADepartamento de Ciencias Biológicas Universidad de los Andes, Carrera 1 No. 18A-10Departamento de Genética e Evolução Universidade Federal de São Carlos (UFSCar campus São Carlos), Rodovia Washington Luiz, km 235Centro de Ciências da Natureza Universidade Federal de São Carlos (UFSCar campus Lagoa do Sino), Rodovia Lauri Simões de Barros, km 12, SP-189Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 AFAPESP: 2007/04073-1FAPESP: 2013/19377-7Universidade Estadual Paulista (Unesp)Universidad de los AndesUniversidade Federal de São Carlos (UFSCar)Pelizzon, Cristiane [UNESP]da Silva Carvalho, Carolina [UNESP]Caballero, SusanaGaletti Junior, Pedro ManoelSanches, Alexandra2018-12-11T17:10:46Z2018-12-11T17:10:46Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-19application/pdfhttp://dx.doi.org/10.1007/s12686-016-0607-yConservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017.1877-72601877-7252http://hdl.handle.net/11449/17436510.1007/s12686-016-0607-y2-s2.0-850159981402-s2.0-85015998140.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengConservation Genetics Resources0,4300,430info:eu-repo/semantics/openAccess2023-10-17T06:03:25Zoai:repositorio.unesp.br:11449/174365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:08.302284Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| title |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| spellingShingle |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples Pelizzon, Cristiane [UNESP] Amelogenin Sexing SRY Tapir ZFX/ZFY |
| title_short |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| title_full |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| title_fullStr |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| title_full_unstemmed |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| title_sort |
Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples |
| author |
Pelizzon, Cristiane [UNESP] |
| author_facet |
Pelizzon, Cristiane [UNESP] da Silva Carvalho, Carolina [UNESP] Caballero, Susana Galetti Junior, Pedro Manoel Sanches, Alexandra |
| author_role |
author |
| author2 |
da Silva Carvalho, Carolina [UNESP] Caballero, Susana Galetti Junior, Pedro Manoel Sanches, Alexandra |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad de los Andes Universidade Federal de São Carlos (UFSCar) |
| dc.contributor.author.fl_str_mv |
Pelizzon, Cristiane [UNESP] da Silva Carvalho, Carolina [UNESP] Caballero, Susana Galetti Junior, Pedro Manoel Sanches, Alexandra |
| dc.subject.por.fl_str_mv |
Amelogenin Sexing SRY Tapir ZFX/ZFY |
| topic |
Amelogenin Sexing SRY Tapir ZFX/ZFY |
| description |
The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:10:46Z 2018-12-11T17:10:46Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12686-016-0607-y Conservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017. 1877-7260 1877-7252 http://hdl.handle.net/11449/174365 10.1007/s12686-016-0607-y 2-s2.0-85015998140 2-s2.0-85015998140.pdf |
| url |
http://dx.doi.org/10.1007/s12686-016-0607-y http://hdl.handle.net/11449/174365 |
| identifier_str_mv |
Conservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017. 1877-7260 1877-7252 10.1007/s12686-016-0607-y 2-s2.0-85015998140 2-s2.0-85015998140.pdf |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Conservation Genetics Resources 0,430 0,430 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
17-19 application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128468257341440 |