Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples

Detalhes bibliográficos
Autor(a) principal: Pelizzon, Cristiane [UNESP]
Data de Publicação: 2017
Outros Autores: da Silva Carvalho, Carolina [UNESP], Caballero, Susana, Galetti Junior, Pedro Manoel, Sanches, Alexandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12686-016-0607-y
http://hdl.handle.net/11449/174365
Resumo: The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs.
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spelling Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samplesAmelogeninSexingSRYTapirZFX/ZFYThe knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 ADepartamento de Ciencias Biológicas Universidad de los Andes, Carrera 1 No. 18A-10Departamento de Genética e Evolução Universidade Federal de São Carlos (UFSCar campus São Carlos), Rodovia Washington Luiz, km 235Centro de Ciências da Natureza Universidade Federal de São Carlos (UFSCar campus Lagoa do Sino), Rodovia Lauri Simões de Barros, km 12, SP-189Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 AFAPESP: 2007/04073-1FAPESP: 2013/19377-7Universidade Estadual Paulista (Unesp)Universidad de los AndesUniversidade Federal de São Carlos (UFSCar)Pelizzon, Cristiane [UNESP]da Silva Carvalho, Carolina [UNESP]Caballero, SusanaGaletti Junior, Pedro ManoelSanches, Alexandra2018-12-11T17:10:46Z2018-12-11T17:10:46Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-19application/pdfhttp://dx.doi.org/10.1007/s12686-016-0607-yConservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017.1877-72601877-7252http://hdl.handle.net/11449/17436510.1007/s12686-016-0607-y2-s2.0-850159981402-s2.0-85015998140.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengConservation Genetics Resources0,4300,430info:eu-repo/semantics/openAccess2024-10-21T12:51:58Zoai:repositorio.unesp.br:11449/174365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-21T12:51:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
title Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
spellingShingle Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
Pelizzon, Cristiane [UNESP]
Amelogenin
Sexing
SRY
Tapir
ZFX/ZFY
title_short Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
title_full Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
title_fullStr Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
title_full_unstemmed Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
title_sort Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
author Pelizzon, Cristiane [UNESP]
author_facet Pelizzon, Cristiane [UNESP]
da Silva Carvalho, Carolina [UNESP]
Caballero, Susana
Galetti Junior, Pedro Manoel
Sanches, Alexandra
author_role author
author2 da Silva Carvalho, Carolina [UNESP]
Caballero, Susana
Galetti Junior, Pedro Manoel
Sanches, Alexandra
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad de los Andes
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Pelizzon, Cristiane [UNESP]
da Silva Carvalho, Carolina [UNESP]
Caballero, Susana
Galetti Junior, Pedro Manoel
Sanches, Alexandra
dc.subject.por.fl_str_mv Amelogenin
Sexing
SRY
Tapir
ZFX/ZFY
topic Amelogenin
Sexing
SRY
Tapir
ZFX/ZFY
description The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
2018-12-11T17:10:46Z
2018-12-11T17:10:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12686-016-0607-y
Conservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017.
1877-7260
1877-7252
http://hdl.handle.net/11449/174365
10.1007/s12686-016-0607-y
2-s2.0-85015998140
2-s2.0-85015998140.pdf
url http://dx.doi.org/10.1007/s12686-016-0607-y
http://hdl.handle.net/11449/174365
identifier_str_mv Conservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017.
1877-7260
1877-7252
10.1007/s12686-016-0607-y
2-s2.0-85015998140
2-s2.0-85015998140.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Conservation Genetics Resources
0,430
0,430
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-19
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1826303641395396608

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