Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado

Detalhes bibliográficos
Autor(a) principal: Mannise, Natalia
Data de Publicação: 2018
Outros Autores: Trovati, R. Guilherme, Duarte, J. Mauricio B. [UNESP], Maldonado, Jesús E., González, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.32800/abc.2018.41.0315
http://hdl.handle.net/11449/221012
Resumo: Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado. The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.
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spelling Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian CerradoUtilización de técnicas genéticas no invasivas para contribuir a la conservación del aguará guazú en un fragment residual del Cerrado de BrasilFecal DNAMicrosatellite lociNeotropical canidReal time PCRZFX–ZFYUsing non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado. The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.Agencia Nacional de Investigación e InnovaciónDepto. de Biodiversidad y Genética Inst. de Investigaciones Biológicas Clemente Estable–MEC, Av. Italia 3318Centro de Energia Nuclear na Agricultura Escola Superior de Agricultura 'Luiz de Queiroz'–Univ. de São Paulo, Av. Centenário 303Núcleo de Pesquisa e Conservação de Cervídeos Depto. de Zootecnia Univ. Estadual Paulista, Via de Acesso Paulo Donato Castellane s/n.Center for Conservation Genomics Smithsonian Conservation Biology Inst. Smithsonian Institution, 3001 Connecticut Ave, NWSección Genética Evolutiva Fac. de Ciencias–UdelaR, Iguá 4225Núcleo de Pesquisa e Conservação de Cervídeos Depto. de Zootecnia Univ. Estadual Paulista, Via de Acesso Paulo Donato Castellane s/n.Agencia Nacional de Investigación e Innovación: 3_2011_1_6619Inst. de Investigaciones Biológicas Clemente Estable–MECEscola Superior de Agricultura 'Luiz de Queiroz'–Univ. de São PauloUniversidade Estadual Paulista (UNESP)Smithsonian InstitutionFac. de Ciencias–UdelaRMannise, NataliaTrovati, R. GuilhermeDuarte, J. Mauricio B. [UNESP]Maldonado, Jesús E.González, Susana2022-04-28T19:08:42Z2022-04-28T19:08:42Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article315-319http://dx.doi.org/10.32800/abc.2018.41.0315Animal Biodiversity and Conservation, v. 41, n. 2, p. 315-319, 2018.2014-928X1578-665Xhttp://hdl.handle.net/11449/22101210.32800/abc.2018.41.03152-s2.0-85041742427Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Biodiversity and Conservationinfo:eu-repo/semantics/openAccess2022-04-28T19:08:42Zoai:repositorio.unesp.br:11449/221012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:08:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
Utilización de técnicas genéticas no invasivas para contribuir a la conservación del aguará guazú en un fragment residual del Cerrado de Brasil
title Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
spellingShingle Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
Mannise, Natalia
Fecal DNA
Microsatellite loci
Neotropical canid
Real time PCR
ZFX–ZFY
title_short Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_full Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_fullStr Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_full_unstemmed Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
title_sort Using non–Invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado
author Mannise, Natalia
author_facet Mannise, Natalia
Trovati, R. Guilherme
Duarte, J. Mauricio B. [UNESP]
Maldonado, Jesús E.
González, Susana
author_role author
author2 Trovati, R. Guilherme
Duarte, J. Mauricio B. [UNESP]
Maldonado, Jesús E.
González, Susana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Inst. de Investigaciones Biológicas Clemente Estable–MEC
Escola Superior de Agricultura 'Luiz de Queiroz'–Univ. de São Paulo
Universidade Estadual Paulista (UNESP)
Smithsonian Institution
Fac. de Ciencias–UdelaR
dc.contributor.author.fl_str_mv Mannise, Natalia
Trovati, R. Guilherme
Duarte, J. Mauricio B. [UNESP]
Maldonado, Jesús E.
González, Susana
dc.subject.por.fl_str_mv Fecal DNA
Microsatellite loci
Neotropical canid
Real time PCR
ZFX–ZFY
topic Fecal DNA
Microsatellite loci
Neotropical canid
Real time PCR
ZFX–ZFY
description Using non–invasive genetic techniques to assist in maned wolf conservation in a remnant fragment of the Brazilian Cerrado. The maned wolf is a South American canid considered a keystone species of the Cerrado. We performed a genetic assessment of maned wolves that inhabit a small remnant fragment of the Cerrado in Brazil. We collected 84 fecal samples over a year and also included two tissue samples from road–killed animals. We successfully identified the species, sex, and individuals using molecular markers. Using microsatellite loci analysis we identified 13 different individuals, eight females and five males. The genetic variability level found and the high number of individuals detected indicates the presence of an open population.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
2022-04-28T19:08:42Z
2022-04-28T19:08:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.32800/abc.2018.41.0315
Animal Biodiversity and Conservation, v. 41, n. 2, p. 315-319, 2018.
2014-928X
1578-665X
http://hdl.handle.net/11449/221012
10.32800/abc.2018.41.0315
2-s2.0-85041742427
url http://dx.doi.org/10.32800/abc.2018.41.0315
http://hdl.handle.net/11449/221012
identifier_str_mv Animal Biodiversity and Conservation, v. 41, n. 2, p. 315-319, 2018.
2014-928X
1578-665X
10.32800/abc.2018.41.0315
2-s2.0-85041742427
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Biodiversity and Conservation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 315-319
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965165389611008

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