Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/183129 |
Resumo: | Sustainable agriculture encourages practices that present low risks to the environment and human health. To this end, zein (corn protein) can be used to develop nanocarrier systems capable of improving the physicochemical properties of biopesticides, reducing their possible toxicity. Neem oil extracted from the Azadirachta indica tree contains many active ingredients including azadirachtin, which is the active ingredient in multiple commercially available biopesticides. In this study, we describe the preparation and characterization of neem oilloaded zein nanoparticles, together with evaluation of their toxicity towards nontarget organisms, using Allium cepa, soil nitrogen cycle microbiota, and Caenorhabditis elegans aiming to achieve the safer by design strategy. The spherical nanoparticles showed an average diameter of 278 ± 61.5 nm and a good stability during the experiments. In the toxicity assays with A. cepa, the neem oil-loaded zein nanoparticles mitigated the increase in the DNA relative damage index caused by the neem oil. Molecular genetic analysis of the soil nitrogen cycle microbiota revealed that neem oil-loaded zein nanoparticles did not change the number of genes which encode nitrogen-fixing enzymes and denitrifying enzymes. In C. elegans, the neem oil-loaded zein nanoparticles had no toxic effect, while neem oil interfered with pharyngeal pumping and GST-4 protein expression. This neem oil-loaded zein nanoparticles showed promising results in the toxicity studies, opening perspectives for its use in crop protection in organic agriculture |
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Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspectiveZein nanoparticleNanopesticideBotanical pesticideAzadirachtinSafer by designSustainable agriculture encourages practices that present low risks to the environment and human health. To this end, zein (corn protein) can be used to develop nanocarrier systems capable of improving the physicochemical properties of biopesticides, reducing their possible toxicity. Neem oil extracted from the Azadirachta indica tree contains many active ingredients including azadirachtin, which is the active ingredient in multiple commercially available biopesticides. In this study, we describe the preparation and characterization of neem oilloaded zein nanoparticles, together with evaluation of their toxicity towards nontarget organisms, using Allium cepa, soil nitrogen cycle microbiota, and Caenorhabditis elegans aiming to achieve the safer by design strategy. The spherical nanoparticles showed an average diameter of 278 ± 61.5 nm and a good stability during the experiments. In the toxicity assays with A. cepa, the neem oil-loaded zein nanoparticles mitigated the increase in the DNA relative damage index caused by the neem oil. Molecular genetic analysis of the soil nitrogen cycle microbiota revealed that neem oil-loaded zein nanoparticles did not change the number of genes which encode nitrogen-fixing enzymes and denitrifying enzymes. In C. elegans, the neem oil-loaded zein nanoparticles had no toxic effect, while neem oil interfered with pharyngeal pumping and GST-4 protein expression. This neem oil-loaded zein nanoparticles showed promising results in the toxicity studies, opening perspectives for its use in crop protection in organic agricultureFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PreprintSão Paulo State University (UNESP), Institute of Science and Technology of Sorocaba, Laboratory of Environmental NanotechnologyResearch Group in Biochemistry and Toxicology in Caenorhabditis elegans, Federal University of PampaLaboratory of Bioactivity Assessment and Toxicology of Nanomaterials, University of SorocabaFAPESP: 2017/21004-5ElsevierUniversidade Estadual Paulista (Unesp)Pascoli, Monica [UNESP]Jacques, Mauricio T.Agarrayua, Danielle A.Avila, Daiana S.Lima, RenataFraceto, Leonardo Fernandes [UNESP]2019-08-05T17:13:06Z2019-08-05T17:13:06Z2019-04-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article57-67application/pdfScience of the Total Environment, v. 677, p. 57-670048-9697http://hdl.handle.net/11449/18312910.1016/j.scitotenv.2019.04.34547414805388833950000-0002-2827-2038enghttp://hdl.handle.net/11449/183274Science of The Total Environmentinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-12-11T06:18:17Zoai:repositorio.unesp.br:11449/183129Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:03:57.738453Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
title |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
spellingShingle |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective Pascoli, Monica [UNESP] Zein nanoparticle Nanopesticide Botanical pesticide Azadirachtin Safer by design |
title_short |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
title_full |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
title_fullStr |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
title_full_unstemmed |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
title_sort |
Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: anecotoxicological perspective |
author |
Pascoli, Monica [UNESP] |
author_facet |
Pascoli, Monica [UNESP] Jacques, Mauricio T. Agarrayua, Danielle A. Avila, Daiana S. Lima, Renata Fraceto, Leonardo Fernandes [UNESP] |
author_role |
author |
author2 |
Jacques, Mauricio T. Agarrayua, Danielle A. Avila, Daiana S. Lima, Renata Fraceto, Leonardo Fernandes [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pascoli, Monica [UNESP] Jacques, Mauricio T. Agarrayua, Danielle A. Avila, Daiana S. Lima, Renata Fraceto, Leonardo Fernandes [UNESP] |
dc.subject.por.fl_str_mv |
Zein nanoparticle Nanopesticide Botanical pesticide Azadirachtin Safer by design |
topic |
Zein nanoparticle Nanopesticide Botanical pesticide Azadirachtin Safer by design |
description |
Sustainable agriculture encourages practices that present low risks to the environment and human health. To this end, zein (corn protein) can be used to develop nanocarrier systems capable of improving the physicochemical properties of biopesticides, reducing their possible toxicity. Neem oil extracted from the Azadirachta indica tree contains many active ingredients including azadirachtin, which is the active ingredient in multiple commercially available biopesticides. In this study, we describe the preparation and characterization of neem oilloaded zein nanoparticles, together with evaluation of their toxicity towards nontarget organisms, using Allium cepa, soil nitrogen cycle microbiota, and Caenorhabditis elegans aiming to achieve the safer by design strategy. The spherical nanoparticles showed an average diameter of 278 ± 61.5 nm and a good stability during the experiments. In the toxicity assays with A. cepa, the neem oil-loaded zein nanoparticles mitigated the increase in the DNA relative damage index caused by the neem oil. Molecular genetic analysis of the soil nitrogen cycle microbiota revealed that neem oil-loaded zein nanoparticles did not change the number of genes which encode nitrogen-fixing enzymes and denitrifying enzymes. In C. elegans, the neem oil-loaded zein nanoparticles had no toxic effect, while neem oil interfered with pharyngeal pumping and GST-4 protein expression. This neem oil-loaded zein nanoparticles showed promising results in the toxicity studies, opening perspectives for its use in crop protection in organic agriculture |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-05T17:13:06Z 2019-08-05T17:13:06Z 2019-04-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Science of the Total Environment, v. 677, p. 57-67 0048-9697 http://hdl.handle.net/11449/183129 10.1016/j.scitotenv.2019.04.345 4741480538883395 0000-0002-2827-2038 |
identifier_str_mv |
Science of the Total Environment, v. 677, p. 57-67 0048-9697 10.1016/j.scitotenv.2019.04.345 4741480538883395 0000-0002-2827-2038 |
url |
http://hdl.handle.net/11449/183129 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://hdl.handle.net/11449/183274 Science of The Total Environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
57-67 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129156314038272 |