Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento

Detalhes bibliográficos
Autor(a) principal: Toledo, Carmen Zilda Pereira de [UNESP]
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/122040
Resumo: The general objective of this study was to describe the inflammatory responses in Pêga Jennies from fresh (NM), extended (ES) and frozen (CS) semen from Jackass. The specific objective was to correlate these responses with low pregnancy rates in Jennies after artificial inseminations (AI) with frozen semen. Twenty five Jennies were daily evaluated by teasing, rectal examination and ultrasound from May 2002 to March 2013. Estrous cycles (n=234) were followed, with 33 (14.22%) non- ovulatory estrous and 11 (4.74%) double ovulations. The estrous cycle length was 32.68±0.76 days, with external heat signs of 4.49±0.23 days and time of ovulation in relationship to end of estrous of 5.68±7.18 hours for Jennies during the observed period. The estrous cycle was 28.88±0.95 days; with external heat signs of 4.50±0.35 days and time to ovulation in relation to end of estrous of 8.0±2.45 hours among 25 Jennies. Thus, considering the obtained results there was no reproductive seasonality. Twelve Jennies were casually selected for biopsy during diestrous being reproductively evaluated and subsequently submitted to one of those: natural mount (NM), AI- ES and AI-CS near the time of ovulations; and then submitted to endometrial biopsy 10 hours latter. Each fragment was maintained in formalin 10%, Bouin solution and Buffer glutaraldehyde 10% for Ligh Microscopy (LM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The endometrial samples from Jennies in diestrous presented folds and endometrial grooves, microvillus cells and ciliated cells, free of process that could compromise reproduction. The estrous samples presented simple columnar epithelium, circled nuclei, condensed chromatin and at the cytoplasm, mitochondrial vacuoles and electro- dense corpuscles. Similar structures to blebs were present at the microvilli cell surface. The endometrial glands were completed by secretions that were shown with major evidence ...
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spelling Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumentoAsininoCiclo estralSemenEndometrio - BiopsiaNeutrofilosEstrous CycleThe general objective of this study was to describe the inflammatory responses in Pêga Jennies from fresh (NM), extended (ES) and frozen (CS) semen from Jackass. The specific objective was to correlate these responses with low pregnancy rates in Jennies after artificial inseminations (AI) with frozen semen. Twenty five Jennies were daily evaluated by teasing, rectal examination and ultrasound from May 2002 to March 2013. Estrous cycles (n=234) were followed, with 33 (14.22%) non- ovulatory estrous and 11 (4.74%) double ovulations. The estrous cycle length was 32.68±0.76 days, with external heat signs of 4.49±0.23 days and time of ovulation in relationship to end of estrous of 5.68±7.18 hours for Jennies during the observed period. The estrous cycle was 28.88±0.95 days; with external heat signs of 4.50±0.35 days and time to ovulation in relation to end of estrous of 8.0±2.45 hours among 25 Jennies. Thus, considering the obtained results there was no reproductive seasonality. Twelve Jennies were casually selected for biopsy during diestrous being reproductively evaluated and subsequently submitted to one of those: natural mount (NM), AI- ES and AI-CS near the time of ovulations; and then submitted to endometrial biopsy 10 hours latter. Each fragment was maintained in formalin 10%, Bouin solution and Buffer glutaraldehyde 10% for Ligh Microscopy (LM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The endometrial samples from Jennies in diestrous presented folds and endometrial grooves, microvillus cells and ciliated cells, free of process that could compromise reproduction. The estrous samples presented simple columnar epithelium, circled nuclei, condensed chromatin and at the cytoplasm, mitochondrial vacuoles and electro- dense corpuscles. Similar structures to blebs were present at the microvilli cell surface. The endometrial glands were completed by secretions that were shown with major evidence ...O objetivo geral deste estudo foi descrever as respostas inflamatórias de jumentas Pêga ao sêmen fresco (MN), sêmen extendido (SE) e sêmen congelado (SC) de jumento, tendo como objetivo específico correlacionar esses achados com os baixos índices de gestações de jumentas Pêga por inseminação artificial (IA) com SC. Foram avaliadas diariamente 25 jumentas, através de rufiações, palpações transretais e exames ultrassonográficos durante maio de 2012 a março de 2013. Foram observados 234 estros, sendo 33 (14,22%) estros anovulatórios e 11 (4,74%) duplas ovulações; o comprimento do ciclo estral foi de 28,88±0,95 dias, com sinais externos de estro de 4,50±0,35 dias e o tempo de ovulação em relação ao término do estro de 8,0±2,45 horas entre as 25 jumentas. Diante dos resultados obtidos, as jumentas não apresentaram sazonalidade reprodutiva. Selecionaram-se 12 jumentas ao acaso, as quais foram biopsiadas em diestro para avaliação reprodutiva e nos ciclos estrais subsequentes, as mesmas foram submetidas à uma das técnicas: monta natural (MN), IA-SE e IA-SC próximo à ovulação, realizando-se biópsia endometrial 10 horas após cada evento. Os fragmentos foram conservados em formalina a 10%, solução de Bouin e solução de glutaraldeído tamponado a 10% para análise em microscopia de luz (ML), microscopia eletrônica de varredura (MEV) e microscopia eletrônica de transmissão (MET). As amostras endometriais das jumentas em diestro apresentaram pregas e sulcos do endométrio, células microvilosas e ciliadas, livre de processos patológicos incompatíveis com a reprodução; em estro apresentaram epitélio colunar simples, evidenciando células microvilosas e células ciliadas, com núcleos ovalados, cromatina condensada e, no citoplasma, presença de mitocôndrias, vacúolos e corpúsculos elétron-densos. Formações semelhantes a bolhas estavam presentes nas superfícies das células microvilosas. As glândulas ...Universidade Estadual Paulista (Unesp)Toniollo, Gilson Hélio [UNESP]Machado, Márcia Rita Fernandes [UNESP]Universidade Estadual Paulista (Unesp)Toledo, Carmen Zilda Pereira de [UNESP]2015-04-09T12:28:18Z2015-04-09T12:28:18Z2014-06-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisxvi, 67 p. : il.application/pdfTOLEDO, Carmen Zilda Pereira de. Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento. 2014. xvi, 67 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.http://hdl.handle.net/11449/122040000815233000815233.pdf33004102069P85604409973209376Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-05T18:01:17Zoai:repositorio.unesp.br:11449/122040Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:48:32.625009Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
title Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
spellingShingle Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
Toledo, Carmen Zilda Pereira de [UNESP]
Asinino
Ciclo estral
Semen
Endometrio - Biopsia
Neutrofilos
Estrous Cycle
title_short Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
title_full Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
title_fullStr Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
title_full_unstemmed Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
title_sort Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento
author Toledo, Carmen Zilda Pereira de [UNESP]
author_facet Toledo, Carmen Zilda Pereira de [UNESP]
author_role author
dc.contributor.none.fl_str_mv Toniollo, Gilson Hélio [UNESP]
Machado, Márcia Rita Fernandes [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Toledo, Carmen Zilda Pereira de [UNESP]
dc.subject.por.fl_str_mv Asinino
Ciclo estral
Semen
Endometrio - Biopsia
Neutrofilos
Estrous Cycle
topic Asinino
Ciclo estral
Semen
Endometrio - Biopsia
Neutrofilos
Estrous Cycle
description The general objective of this study was to describe the inflammatory responses in Pêga Jennies from fresh (NM), extended (ES) and frozen (CS) semen from Jackass. The specific objective was to correlate these responses with low pregnancy rates in Jennies after artificial inseminations (AI) with frozen semen. Twenty five Jennies were daily evaluated by teasing, rectal examination and ultrasound from May 2002 to March 2013. Estrous cycles (n=234) were followed, with 33 (14.22%) non- ovulatory estrous and 11 (4.74%) double ovulations. The estrous cycle length was 32.68±0.76 days, with external heat signs of 4.49±0.23 days and time of ovulation in relationship to end of estrous of 5.68±7.18 hours for Jennies during the observed period. The estrous cycle was 28.88±0.95 days; with external heat signs of 4.50±0.35 days and time to ovulation in relation to end of estrous of 8.0±2.45 hours among 25 Jennies. Thus, considering the obtained results there was no reproductive seasonality. Twelve Jennies were casually selected for biopsy during diestrous being reproductively evaluated and subsequently submitted to one of those: natural mount (NM), AI- ES and AI-CS near the time of ovulations; and then submitted to endometrial biopsy 10 hours latter. Each fragment was maintained in formalin 10%, Bouin solution and Buffer glutaraldehyde 10% for Ligh Microscopy (LM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The endometrial samples from Jennies in diestrous presented folds and endometrial grooves, microvillus cells and ciliated cells, free of process that could compromise reproduction. The estrous samples presented simple columnar epithelium, circled nuclei, condensed chromatin and at the cytoplasm, mitochondrial vacuoles and electro- dense corpuscles. Similar structures to blebs were present at the microvilli cell surface. The endometrial glands were completed by secretions that were shown with major evidence ...
publishDate 2014
dc.date.none.fl_str_mv 2014-06-18
2015-04-09T12:28:18Z
2015-04-09T12:28:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv TOLEDO, Carmen Zilda Pereira de. Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento. 2014. xvi, 67 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.
http://hdl.handle.net/11449/122040
000815233
000815233.pdf
33004102069P8
5604409973209376
identifier_str_mv TOLEDO, Carmen Zilda Pereira de. Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento. 2014. xvi, 67 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.
000815233
000815233.pdf
33004102069P8
5604409973209376
url http://hdl.handle.net/11449/122040
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv xvi, 67 p. : il.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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