Double effort test for evaluation of aerobic capacity of diet-induced obese rats

Detalhes bibliográficos
Autor(a) principal: Brandão, Bruna Brasil [UNESP]
Data de Publicação: 2013
Outros Autores: de Souza, Lidiane Moreira [UNESP], Trombetta, Beatriz de Novais Oliveira [UNESP], Pastre, Carlos Marcelo [UNESP], Papoti, Marcelo, Seraphim, Patricia Monteiro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-86922013000300014
http://hdl.handle.net/11449/76499
Resumo: Introduction: The literature lacks studies about lactate actions and some limitations in studies involving healthy individuals or patients with some metabolic disorder. Objectives: This study aimed to evaluate the protocol of double effort test for obese-induced rats. Methods: Fourteen male Wistar rats were divided into two groups: Control (Con) and Obese (Obe). The control group was fed with standard chow and water ad libitum. The obese group was fed with standard chow, water ad libitum and hyperlipidic diet. Twelve weeks after the beginning of the hyperlipidic diet, insulin tolerance test, Maximal Lactate Steady State (MLSS) test and the double efforts test were performed. Results: The diet was effective to promote obesity. The obese group decreased insulin sensitivity in approximately 19% (Con = 2.156 ± 0.1187 AU vs Obe = 1.742 ± 0.1551 AU). The lactate concentration and velocity of anaerobic threshold at MLSS test were 3.780 ± 0.09 mmol/L e 18 m.min-1 in both groups. The velocity of anaerobic threshold estimated by double efforts test was 15.59±0.653 m.min-1 in Con group control animals and 16.42±0.672 m.min-1 in Obe group. The double effort test underestimated around 13% and 8.7% the aerobic capacity in control and obese groups respectively, however, presented significant correlation with MLSS (r = 0,88; P < 0,0075 controls / r = 0,92; P < 0,0031 obese). Conclusion: So, the double effort test can be an interesting alternative to evaluate the aerobic capacity for both healthy sedentary and obese animals.
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spelling Double effort test for evaluation of aerobic capacity of diet-induced obese ratsLactic acidObesityRunningIntroduction: The literature lacks studies about lactate actions and some limitations in studies involving healthy individuals or patients with some metabolic disorder. Objectives: This study aimed to evaluate the protocol of double effort test for obese-induced rats. Methods: Fourteen male Wistar rats were divided into two groups: Control (Con) and Obese (Obe). The control group was fed with standard chow and water ad libitum. The obese group was fed with standard chow, water ad libitum and hyperlipidic diet. Twelve weeks after the beginning of the hyperlipidic diet, insulin tolerance test, Maximal Lactate Steady State (MLSS) test and the double efforts test were performed. Results: The diet was effective to promote obesity. The obese group decreased insulin sensitivity in approximately 19% (Con = 2.156 ± 0.1187 AU vs Obe = 1.742 ± 0.1551 AU). The lactate concentration and velocity of anaerobic threshold at MLSS test were 3.780 ± 0.09 mmol/L e 18 m.min-1 in both groups. The velocity of anaerobic threshold estimated by double efforts test was 15.59±0.653 m.min-1 in Con group control animals and 16.42±0.672 m.min-1 in Obe group. The double effort test underestimated around 13% and 8.7% the aerobic capacity in control and obese groups respectively, however, presented significant correlation with MLSS (r = 0,88; P < 0,0075 controls / r = 0,92; P < 0,0031 obese). Conclusion: So, the double effort test can be an interesting alternative to evaluate the aerobic capacity for both healthy sedentary and obese animals.Department of Physiotherapy Laboratory of Research in Physiology State University of São Paulo, Júlio de Mesquita FilhoPhysical Education and Sports School of Ribeirão Preto USPDepartment of Physiotherapy Laboratory of Research in Physiology State University of São Paulo, Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Brandão, Bruna Brasil [UNESP]de Souza, Lidiane Moreira [UNESP]Trombetta, Beatriz de Novais Oliveira [UNESP]Pastre, Carlos Marcelo [UNESP]Papoti, MarceloSeraphim, Patricia Monteiro [UNESP]2014-05-27T11:30:37Z2014-05-27T11:30:37Z2013-09-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article214-218application/pdfhttp://dx.doi.org/10.1590/S1517-86922013000300014Revista Brasileira de Medicina do Esporte, v. 19, n. 3, p. 214-218, 2013.1517-8692http://hdl.handle.net/11449/7649910.1590/S1517-86922013000300014S1517-86922013000300014WOS:0003235010000142-s2.0-848830688222-s2.0-84883068822.pdf276139654405860604110085990708710000-0003-2145-6640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Medicina do Esporte0.2700,185info:eu-repo/semantics/openAccess2024-06-18T18:44:03Zoai:repositorio.unesp.br:11449/76499Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:54:43.116789Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Double effort test for evaluation of aerobic capacity of diet-induced obese rats
title Double effort test for evaluation of aerobic capacity of diet-induced obese rats
spellingShingle Double effort test for evaluation of aerobic capacity of diet-induced obese rats
Brandão, Bruna Brasil [UNESP]
Lactic acid
Obesity
Running
title_short Double effort test for evaluation of aerobic capacity of diet-induced obese rats
title_full Double effort test for evaluation of aerobic capacity of diet-induced obese rats
title_fullStr Double effort test for evaluation of aerobic capacity of diet-induced obese rats
title_full_unstemmed Double effort test for evaluation of aerobic capacity of diet-induced obese rats
title_sort Double effort test for evaluation of aerobic capacity of diet-induced obese rats
author Brandão, Bruna Brasil [UNESP]
author_facet Brandão, Bruna Brasil [UNESP]
de Souza, Lidiane Moreira [UNESP]
Trombetta, Beatriz de Novais Oliveira [UNESP]
Pastre, Carlos Marcelo [UNESP]
Papoti, Marcelo
Seraphim, Patricia Monteiro [UNESP]
author_role author
author2 de Souza, Lidiane Moreira [UNESP]
Trombetta, Beatriz de Novais Oliveira [UNESP]
Pastre, Carlos Marcelo [UNESP]
Papoti, Marcelo
Seraphim, Patricia Monteiro [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Brandão, Bruna Brasil [UNESP]
de Souza, Lidiane Moreira [UNESP]
Trombetta, Beatriz de Novais Oliveira [UNESP]
Pastre, Carlos Marcelo [UNESP]
Papoti, Marcelo
Seraphim, Patricia Monteiro [UNESP]
dc.subject.por.fl_str_mv Lactic acid
Obesity
Running
topic Lactic acid
Obesity
Running
description Introduction: The literature lacks studies about lactate actions and some limitations in studies involving healthy individuals or patients with some metabolic disorder. Objectives: This study aimed to evaluate the protocol of double effort test for obese-induced rats. Methods: Fourteen male Wistar rats were divided into two groups: Control (Con) and Obese (Obe). The control group was fed with standard chow and water ad libitum. The obese group was fed with standard chow, water ad libitum and hyperlipidic diet. Twelve weeks after the beginning of the hyperlipidic diet, insulin tolerance test, Maximal Lactate Steady State (MLSS) test and the double efforts test were performed. Results: The diet was effective to promote obesity. The obese group decreased insulin sensitivity in approximately 19% (Con = 2.156 ± 0.1187 AU vs Obe = 1.742 ± 0.1551 AU). The lactate concentration and velocity of anaerobic threshold at MLSS test were 3.780 ± 0.09 mmol/L e 18 m.min-1 in both groups. The velocity of anaerobic threshold estimated by double efforts test was 15.59±0.653 m.min-1 in Con group control animals and 16.42±0.672 m.min-1 in Obe group. The double effort test underestimated around 13% and 8.7% the aerobic capacity in control and obese groups respectively, however, presented significant correlation with MLSS (r = 0,88; P < 0,0075 controls / r = 0,92; P < 0,0031 obese). Conclusion: So, the double effort test can be an interesting alternative to evaluate the aerobic capacity for both healthy sedentary and obese animals.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-02
2014-05-27T11:30:37Z
2014-05-27T11:30:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-86922013000300014
Revista Brasileira de Medicina do Esporte, v. 19, n. 3, p. 214-218, 2013.
1517-8692
http://hdl.handle.net/11449/76499
10.1590/S1517-86922013000300014
S1517-86922013000300014
WOS:000323501000014
2-s2.0-84883068822
2-s2.0-84883068822.pdf
2761396544058606
0411008599070871
0000-0003-2145-6640
url http://dx.doi.org/10.1590/S1517-86922013000300014
http://hdl.handle.net/11449/76499
identifier_str_mv Revista Brasileira de Medicina do Esporte, v. 19, n. 3, p. 214-218, 2013.
1517-8692
10.1590/S1517-86922013000300014
S1517-86922013000300014
WOS:000323501000014
2-s2.0-84883068822
2-s2.0-84883068822.pdf
2761396544058606
0411008599070871
0000-0003-2145-6640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Medicina do Esporte
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0,185
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 214-218
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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