Laccases produced by Peniophora from marine and terrestrial origin: A comparative study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bcab.2021.102066 http://hdl.handle.net/11449/233185 |
Resumo: | Although fungal laccases have been widely applied in environmental and industrial processes, little is known about laccases produced by marine fungi. In this study, the laccases from basidiomycetes of marine (Peniophora sp. CBMAI 1063) and terrestrial (Peniophora cinerea CCIBt 2541) origin were compared. Using crossflow filtration and anionic exchange chromatography, these laccases were partially purified and characterized. The marine fungus produced two laccase isoforms, whereas the terrestrial strain produced five. The enzymatic pool of the terrestrial Peniophora showed higher affinity (KM) with the tested substrates. On the other hand, laccases from the marine fungus presented higher thermal resistance. The optimum temperature (55 °C) and pH values (4.0 with ABTS and 5.5 with SYG) were the same for both fungi. No significant difference was observed in the reactive blue dye decolorization rates (from 61.17 to 67.38%) after incubation with the laccases from both Peniophora strains and the pure commercial laccase from Trametes versicolor. The increase in toxicity only observed in the experiments using the laccases from the marine Peniophora strain indicates different decolorization mechanisms. This is the first report on reactive blue textile dye decolorization by laccases from the genus Peniophora, expanding their potential for biotechnological application. In this study, the adaptation of the Peniophora strains to the environments from where they were isolated was evidenced, and differences in their laccase isoforms and enzymatic characteristics were observed. The results highlight the advantage of the marine-derived fungus for laccase production under saline conditions and improve the knowledge on marine enzymes. |
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Laccases produced by Peniophora from marine and terrestrial origin: A comparative studyAnionic exchange chromatographyBasidiomycetesBioremediationCrossflow filtrationEnzyme kineticsMarine enzymesAlthough fungal laccases have been widely applied in environmental and industrial processes, little is known about laccases produced by marine fungi. In this study, the laccases from basidiomycetes of marine (Peniophora sp. CBMAI 1063) and terrestrial (Peniophora cinerea CCIBt 2541) origin were compared. Using crossflow filtration and anionic exchange chromatography, these laccases were partially purified and characterized. The marine fungus produced two laccase isoforms, whereas the terrestrial strain produced five. The enzymatic pool of the terrestrial Peniophora showed higher affinity (KM) with the tested substrates. On the other hand, laccases from the marine fungus presented higher thermal resistance. The optimum temperature (55 °C) and pH values (4.0 with ABTS and 5.5 with SYG) were the same for both fungi. No significant difference was observed in the reactive blue dye decolorization rates (from 61.17 to 67.38%) after incubation with the laccases from both Peniophora strains and the pure commercial laccase from Trametes versicolor. The increase in toxicity only observed in the experiments using the laccases from the marine Peniophora strain indicates different decolorization mechanisms. This is the first report on reactive blue textile dye decolorization by laccases from the genus Peniophora, expanding their potential for biotechnological application. In this study, the adaptation of the Peniophora strains to the environments from where they were isolated was evidenced, and differences in their laccase isoforms and enzymatic characteristics were observed. The results highlight the advantage of the marine-derived fungus for laccase production under saline conditions and improve the knowledge on marine enzymes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Geral e Aplicada Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av 24A, 1515Departamento de Tecnologia Bioquímico-Farmacêutica Faculdade de Ciências Farmacêuticas Universidade de São Paulo (USP), Av Prof. Lineu Prestes, 580 - Bloco 16 – ButantãDepartamento de Biologia Geral e Aplicada Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av 24A, 1515FAPESP: 2016/07957-7Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)de Jesus Fontes, Bruno [UNESP]Kleingesinds, Eduardo KrebsGiovanella, Patricia [UNESP]Junior, Adalberto PessoaSette, Lara Durães [UNESP]2022-05-01T05:29:32Z2022-05-01T05:29:32Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bcab.2021.102066Biocatalysis and Agricultural Biotechnology, v. 35.1878-8181http://hdl.handle.net/11449/23318510.1016/j.bcab.2021.1020662-s2.0-85108519410Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocatalysis and Agricultural Biotechnologyinfo:eu-repo/semantics/openAccess2022-05-01T05:29:32Zoai:repositorio.unesp.br:11449/233185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T05:29:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
title |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
spellingShingle |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study de Jesus Fontes, Bruno [UNESP] Anionic exchange chromatography Basidiomycetes Bioremediation Crossflow filtration Enzyme kinetics Marine enzymes |
title_short |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
title_full |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
title_fullStr |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
title_full_unstemmed |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
title_sort |
Laccases produced by Peniophora from marine and terrestrial origin: A comparative study |
author |
de Jesus Fontes, Bruno [UNESP] |
author_facet |
de Jesus Fontes, Bruno [UNESP] Kleingesinds, Eduardo Krebs Giovanella, Patricia [UNESP] Junior, Adalberto Pessoa Sette, Lara Durães [UNESP] |
author_role |
author |
author2 |
Kleingesinds, Eduardo Krebs Giovanella, Patricia [UNESP] Junior, Adalberto Pessoa Sette, Lara Durães [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Jesus Fontes, Bruno [UNESP] Kleingesinds, Eduardo Krebs Giovanella, Patricia [UNESP] Junior, Adalberto Pessoa Sette, Lara Durães [UNESP] |
dc.subject.por.fl_str_mv |
Anionic exchange chromatography Basidiomycetes Bioremediation Crossflow filtration Enzyme kinetics Marine enzymes |
topic |
Anionic exchange chromatography Basidiomycetes Bioremediation Crossflow filtration Enzyme kinetics Marine enzymes |
description |
Although fungal laccases have been widely applied in environmental and industrial processes, little is known about laccases produced by marine fungi. In this study, the laccases from basidiomycetes of marine (Peniophora sp. CBMAI 1063) and terrestrial (Peniophora cinerea CCIBt 2541) origin were compared. Using crossflow filtration and anionic exchange chromatography, these laccases were partially purified and characterized. The marine fungus produced two laccase isoforms, whereas the terrestrial strain produced five. The enzymatic pool of the terrestrial Peniophora showed higher affinity (KM) with the tested substrates. On the other hand, laccases from the marine fungus presented higher thermal resistance. The optimum temperature (55 °C) and pH values (4.0 with ABTS and 5.5 with SYG) were the same for both fungi. No significant difference was observed in the reactive blue dye decolorization rates (from 61.17 to 67.38%) after incubation with the laccases from both Peniophora strains and the pure commercial laccase from Trametes versicolor. The increase in toxicity only observed in the experiments using the laccases from the marine Peniophora strain indicates different decolorization mechanisms. This is the first report on reactive blue textile dye decolorization by laccases from the genus Peniophora, expanding their potential for biotechnological application. In this study, the adaptation of the Peniophora strains to the environments from where they were isolated was evidenced, and differences in their laccase isoforms and enzymatic characteristics were observed. The results highlight the advantage of the marine-derived fungus for laccase production under saline conditions and improve the knowledge on marine enzymes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-05-01T05:29:32Z 2022-05-01T05:29:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bcab.2021.102066 Biocatalysis and Agricultural Biotechnology, v. 35. 1878-8181 http://hdl.handle.net/11449/233185 10.1016/j.bcab.2021.102066 2-s2.0-85108519410 |
url |
http://dx.doi.org/10.1016/j.bcab.2021.102066 http://hdl.handle.net/11449/233185 |
identifier_str_mv |
Biocatalysis and Agricultural Biotechnology, v. 35. 1878-8181 10.1016/j.bcab.2021.102066 2-s2.0-85108519410 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biocatalysis and Agricultural Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650384941547520 |