Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016000600001 http://hdl.handle.net/11449/173362 |
Resumo: | The platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects. |
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Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equinoPRP-gel scaffold associated with mesenchymal stem cells: Use in experimental chondral defect of equine modelsEquineFibrin gelMesenchymal stem cellsPlatelet-rich plasmaPRP-gelScaffoldStem cellsThe platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects.Departamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nUniversidade Estadual Paulista (Unesp)Yamada, Ana Lúcia M. [UNESP]Alvarenga, Marina L. [UNESP]Brandão, Jaqueline S. [UNESP]Watanabe, Marcos J. [UNESP]Rodrigues, Celso A. [UNESP]Hussni, Carlos A. [UNESP]Alves, Ana L.G. [UNESP]2018-12-11T17:04:49Z2018-12-11T17:04:49Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article461-467application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016000600001Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/17336210.1590/S0100-736X2016000600001S0100-736X20160006004612-s2.0-84982296134S0100-736X2016000600461.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-10-30T06:14:00Zoai:repositorio.unesp.br:11449/173362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:29:43.136228Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino PRP-gel scaffold associated with mesenchymal stem cells: Use in experimental chondral defect of equine models |
title |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
spellingShingle |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino Yamada, Ana Lúcia M. [UNESP] Equine Fibrin gel Mesenchymal stem cells Platelet-rich plasma PRP-gel Scaffold Stem cells |
title_short |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
title_full |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
title_fullStr |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
title_full_unstemmed |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
title_sort |
Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino |
author |
Yamada, Ana Lúcia M. [UNESP] |
author_facet |
Yamada, Ana Lúcia M. [UNESP] Alvarenga, Marina L. [UNESP] Brandão, Jaqueline S. [UNESP] Watanabe, Marcos J. [UNESP] Rodrigues, Celso A. [UNESP] Hussni, Carlos A. [UNESP] Alves, Ana L.G. [UNESP] |
author_role |
author |
author2 |
Alvarenga, Marina L. [UNESP] Brandão, Jaqueline S. [UNESP] Watanabe, Marcos J. [UNESP] Rodrigues, Celso A. [UNESP] Hussni, Carlos A. [UNESP] Alves, Ana L.G. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Yamada, Ana Lúcia M. [UNESP] Alvarenga, Marina L. [UNESP] Brandão, Jaqueline S. [UNESP] Watanabe, Marcos J. [UNESP] Rodrigues, Celso A. [UNESP] Hussni, Carlos A. [UNESP] Alves, Ana L.G. [UNESP] |
dc.subject.por.fl_str_mv |
Equine Fibrin gel Mesenchymal stem cells Platelet-rich plasma PRP-gel Scaffold Stem cells |
topic |
Equine Fibrin gel Mesenchymal stem cells Platelet-rich plasma PRP-gel Scaffold Stem cells |
description |
The platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:04:49Z 2018-12-11T17:04:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016000600001 Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016. 1678-5150 0100-736X http://hdl.handle.net/11449/173362 10.1590/S0100-736X2016000600001 S0100-736X2016000600461 2-s2.0-84982296134 S0100-736X2016000600461.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016000600001 http://hdl.handle.net/11449/173362 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016. 1678-5150 0100-736X 10.1590/S0100-736X2016000600001 S0100-736X2016000600461 2-s2.0-84982296134 S0100-736X2016000600461.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
461-467 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128660343881728 |