Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino

Detalhes bibliográficos
Autor(a) principal: Yamada, Ana Lúcia M. [UNESP]
Data de Publicação: 2016
Outros Autores: Alvarenga, Marina L. [UNESP], Brandão, Jaqueline S. [UNESP], Watanabe, Marcos J. [UNESP], Rodrigues, Celso A. [UNESP], Hussni, Carlos A. [UNESP], Alves, Ana L.G. [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2016000600001
http://hdl.handle.net/11449/173362
Resumo: The platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects.
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spelling Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equinoPRP-gel scaffold associated with mesenchymal stem cells: Use in experimental chondral defect of equine modelsEquineFibrin gelMesenchymal stem cellsPlatelet-rich plasmaPRP-gelScaffoldStem cellsThe platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects.Departamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Cirurgia e Anestesiologia Veterinária Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nUniversidade Estadual Paulista (Unesp)Yamada, Ana Lúcia M. [UNESP]Alvarenga, Marina L. [UNESP]Brandão, Jaqueline S. [UNESP]Watanabe, Marcos J. [UNESP]Rodrigues, Celso A. [UNESP]Hussni, Carlos A. [UNESP]Alves, Ana L.G. [UNESP]2018-12-11T17:04:49Z2018-12-11T17:04:49Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article461-467application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016000600001Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/17336210.1590/S0100-736X2016000600001S0100-736X20160006004612-s2.0-84982296134S0100-736X2016000600461.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-10-30T06:14:00Zoai:repositorio.unesp.br:11449/173362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:29:43.136228Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
PRP-gel scaffold associated with mesenchymal stem cells: Use in experimental chondral defect of equine models
title Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
spellingShingle Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
Yamada, Ana Lúcia M. [UNESP]
Equine
Fibrin gel
Mesenchymal stem cells
Platelet-rich plasma
PRP-gel
Scaffold
Stem cells
title_short Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
title_full Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
title_fullStr Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
title_full_unstemmed Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
title_sort Arcabouço de PRP-gel associado a células tronco mesenquimais: Uso em lesões condrais em modelo experimental equino
author Yamada, Ana Lúcia M. [UNESP]
author_facet Yamada, Ana Lúcia M. [UNESP]
Alvarenga, Marina L. [UNESP]
Brandão, Jaqueline S. [UNESP]
Watanabe, Marcos J. [UNESP]
Rodrigues, Celso A. [UNESP]
Hussni, Carlos A. [UNESP]
Alves, Ana L.G. [UNESP]
author_role author
author2 Alvarenga, Marina L. [UNESP]
Brandão, Jaqueline S. [UNESP]
Watanabe, Marcos J. [UNESP]
Rodrigues, Celso A. [UNESP]
Hussni, Carlos A. [UNESP]
Alves, Ana L.G. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Yamada, Ana Lúcia M. [UNESP]
Alvarenga, Marina L. [UNESP]
Brandão, Jaqueline S. [UNESP]
Watanabe, Marcos J. [UNESP]
Rodrigues, Celso A. [UNESP]
Hussni, Carlos A. [UNESP]
Alves, Ana L.G. [UNESP]
dc.subject.por.fl_str_mv Equine
Fibrin gel
Mesenchymal stem cells
Platelet-rich plasma
PRP-gel
Scaffold
Stem cells
topic Equine
Fibrin gel
Mesenchymal stem cells
Platelet-rich plasma
PRP-gel
Scaffold
Stem cells
description The platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:04:49Z
2018-12-11T17:04:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2016000600001
Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016.
1678-5150
0100-736X
http://hdl.handle.net/11449/173362
10.1590/S0100-736X2016000600001
S0100-736X2016000600461
2-s2.0-84982296134
S0100-736X2016000600461.pdf
url http://dx.doi.org/10.1590/S0100-736X2016000600001
http://hdl.handle.net/11449/173362
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 36, n. 6, p. 461-467, 2016.
1678-5150
0100-736X
10.1590/S0100-736X2016000600001
S0100-736X2016000600461
2-s2.0-84982296134
S0100-736X2016000600461.pdf
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 461-467
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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