Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00403-016-1676-1 http://hdl.handle.net/11449/178138 |
Resumo: | The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo. |
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Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitroFibroblastsKeratinocytesMesenchymal stem cellsPlatelet-rich plasmaThe clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.University of São Paulo USPUniversity of Campinas UNICAMPCordCell Cell TherapySão Paulo State University UNESP, Campus de AssisDepartment of Biological Sciences São Paulo State University UNESP, Av. Dom Antonio, 2100São Paulo State University UNESP, Campus de AssisDepartment of Biological Sciences São Paulo State University UNESP, Av. Dom Antonio, 2100Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)CordCell Cell TherapyUniversidade Estadual Paulista (Unesp)Stessuk, TalitaPuzzi, Maria BeatrizChaim, Elinton AdamiAlves, Paulo César Martinsde Paula, Erich ViniciusForte, AndresaIzumizawa, Juliana MassaeOliveira, Carolina CaliáriFrei, Fernando [UNESP]Ribeiro-Paes, João Tadeu [UNESP]2018-12-11T17:28:56Z2018-12-11T17:28:56Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article511-520application/pdfhttp://dx.doi.org/10.1007/s00403-016-1676-1Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016.1432-069X0340-3696http://hdl.handle.net/11449/17813810.1007/s00403-016-1676-12-s2.0-849780362782-s2.0-84978036278.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Dermatological Research1,006info:eu-repo/semantics/openAccess2023-11-17T06:14:50Zoai:repositorio.unesp.br:11449/178138Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-17T06:14:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
title |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
spellingShingle |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro Stessuk, Talita Fibroblasts Keratinocytes Mesenchymal stem cells Platelet-rich plasma |
title_short |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
title_full |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
title_fullStr |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
title_full_unstemmed |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
title_sort |
Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro |
author |
Stessuk, Talita |
author_facet |
Stessuk, Talita Puzzi, Maria Beatriz Chaim, Elinton Adami Alves, Paulo César Martins de Paula, Erich Vinicius Forte, Andresa Izumizawa, Juliana Massae Oliveira, Carolina Caliári Frei, Fernando [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
author_role |
author |
author2 |
Puzzi, Maria Beatriz Chaim, Elinton Adami Alves, Paulo César Martins de Paula, Erich Vinicius Forte, Andresa Izumizawa, Juliana Massae Oliveira, Carolina Caliári Frei, Fernando [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) CordCell Cell Therapy Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Stessuk, Talita Puzzi, Maria Beatriz Chaim, Elinton Adami Alves, Paulo César Martins de Paula, Erich Vinicius Forte, Andresa Izumizawa, Juliana Massae Oliveira, Carolina Caliári Frei, Fernando [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
dc.subject.por.fl_str_mv |
Fibroblasts Keratinocytes Mesenchymal stem cells Platelet-rich plasma |
topic |
Fibroblasts Keratinocytes Mesenchymal stem cells Platelet-rich plasma |
description |
The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:28:56Z 2018-12-11T17:28:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00403-016-1676-1 Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016. 1432-069X 0340-3696 http://hdl.handle.net/11449/178138 10.1007/s00403-016-1676-1 2-s2.0-84978036278 2-s2.0-84978036278.pdf |
url |
http://dx.doi.org/10.1007/s00403-016-1676-1 http://hdl.handle.net/11449/178138 |
identifier_str_mv |
Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016. 1432-069X 0340-3696 10.1007/s00403-016-1676-1 2-s2.0-84978036278 2-s2.0-84978036278.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Dermatological Research 1,006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
511-520 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964972576407552 |