Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro

Detalhes bibliográficos
Autor(a) principal: Stessuk, Talita
Data de Publicação: 2016
Outros Autores: Puzzi, Maria Beatriz, Chaim, Elinton Adami, Alves, Paulo César Martins, de Paula, Erich Vinicius, Forte, Andresa, Izumizawa, Juliana Massae, Oliveira, Carolina Caliári, Frei, Fernando [UNESP], Ribeiro-Paes, João Tadeu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00403-016-1676-1
http://hdl.handle.net/11449/178138
Resumo: The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.
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spelling Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitroFibroblastsKeratinocytesMesenchymal stem cellsPlatelet-rich plasmaThe clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.University of São Paulo USPUniversity of Campinas UNICAMPCordCell Cell TherapySão Paulo State University UNESP, Campus de AssisDepartment of Biological Sciences São Paulo State University UNESP, Av. Dom Antonio, 2100São Paulo State University UNESP, Campus de AssisDepartment of Biological Sciences São Paulo State University UNESP, Av. Dom Antonio, 2100Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)CordCell Cell TherapyUniversidade Estadual Paulista (Unesp)Stessuk, TalitaPuzzi, Maria BeatrizChaim, Elinton AdamiAlves, Paulo César Martinsde Paula, Erich ViniciusForte, AndresaIzumizawa, Juliana MassaeOliveira, Carolina CaliáriFrei, Fernando [UNESP]Ribeiro-Paes, João Tadeu [UNESP]2018-12-11T17:28:56Z2018-12-11T17:28:56Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article511-520application/pdfhttp://dx.doi.org/10.1007/s00403-016-1676-1Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016.1432-069X0340-3696http://hdl.handle.net/11449/17813810.1007/s00403-016-1676-12-s2.0-849780362782-s2.0-84978036278.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Dermatological Research1,006info:eu-repo/semantics/openAccess2023-11-17T06:14:50Zoai:repositorio.unesp.br:11449/178138Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-17T06:14:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
title Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
spellingShingle Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
Stessuk, Talita
Fibroblasts
Keratinocytes
Mesenchymal stem cells
Platelet-rich plasma
title_short Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
title_full Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
title_fullStr Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
title_full_unstemmed Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
title_sort Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
author Stessuk, Talita
author_facet Stessuk, Talita
Puzzi, Maria Beatriz
Chaim, Elinton Adami
Alves, Paulo César Martins
de Paula, Erich Vinicius
Forte, Andresa
Izumizawa, Juliana Massae
Oliveira, Carolina Caliári
Frei, Fernando [UNESP]
Ribeiro-Paes, João Tadeu [UNESP]
author_role author
author2 Puzzi, Maria Beatriz
Chaim, Elinton Adami
Alves, Paulo César Martins
de Paula, Erich Vinicius
Forte, Andresa
Izumizawa, Juliana Massae
Oliveira, Carolina Caliári
Frei, Fernando [UNESP]
Ribeiro-Paes, João Tadeu [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
CordCell Cell Therapy
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Stessuk, Talita
Puzzi, Maria Beatriz
Chaim, Elinton Adami
Alves, Paulo César Martins
de Paula, Erich Vinicius
Forte, Andresa
Izumizawa, Juliana Massae
Oliveira, Carolina Caliári
Frei, Fernando [UNESP]
Ribeiro-Paes, João Tadeu [UNESP]
dc.subject.por.fl_str_mv Fibroblasts
Keratinocytes
Mesenchymal stem cells
Platelet-rich plasma
topic Fibroblasts
Keratinocytes
Mesenchymal stem cells
Platelet-rich plasma
description The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T17:28:56Z
2018-12-11T17:28:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00403-016-1676-1
Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016.
1432-069X
0340-3696
http://hdl.handle.net/11449/178138
10.1007/s00403-016-1676-1
2-s2.0-84978036278
2-s2.0-84978036278.pdf
url http://dx.doi.org/10.1007/s00403-016-1676-1
http://hdl.handle.net/11449/178138
identifier_str_mv Archives of Dermatological Research, v. 308, n. 7, p. 511-520, 2016.
1432-069X
0340-3696
10.1007/s00403-016-1676-1
2-s2.0-84978036278
2-s2.0-84978036278.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Dermatological Research
1,006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 511-520
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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