Produção de lacase por fungo isolado de sedimento marinho. -

• Autor(a) principal: Paiva, Cindy Kurovski de Castro [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155118; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-03-07/000879045.pdf
• topic: Fungos; Biorremediação; Enzimas; Planejamento experimental

Filamentous fungi and their metabolites have been prominent in the processes of bioremediation due to the high degradation potential, biosorption, and mechanisms of resistance in adverse environmental conditions. One of the most important ligninolytic enzymes is laccase (benzenodiol: oxygen oxidoreductase; EC 1.10.3.2). Laccases are oxidases which catalyze the oxidation of a broad spectrum of substrates, such as ortho- and para-diphenols, polyphenols, aminophenols, and aromatic or aliphatic amines. These enzymes act on both phenolic and nonphenolic lignin-related compounds as well as highly recalcitrant environmental pollutants, and they can be effectively used in different industrial sectors. Based on the industrial and environmental importance of laccases, the objective of this work was to evaluate different factors in the production of laccase by the marine-derived fungus LAMAI 659, aiming to optimize the production process. For that end, experimental design based on statistical principles was used, following the Plackett & Burmann model. The present work was developed considering the previous studies carried out by the research group coordinated by the Prof. Dr. Lara Sette (IB-UNESP / Rio Claro). For the structure of the assays, the fungus was reactivated in solid MA2 medium (2% malt extract and 2% agar) at 28ºC for 7 days and used as inocula. Assays were incubated for 7 days at 140 rpm and 25 °C. Laccase activity was detected by absorbance reading during the oxidation of the ABTS substrate (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid). The influence of the variables studied on laccase production was analyzed using the software STATISTICA 7.0, being the assays that presented the best results of enzymatic production selected for validation of the culture conditions. The first experimental design (1st P&B)... (Complete abstract electronic acess below)