Aplicação de consórcio microbiano marinho na destoxificação do contaminante pireno

• Autor(a) principal: Leme, Carla de Rezende [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155120; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-03-07/000879056.pdf
• topic: Biodegradação; Biorremediação; Planejamento experimental; Ecologia microbiana; Enzimas

Several previous studies related to the environmental microbiology have been demonstrating the potencial presented by microorganisms to degrade and detoxify environments contaminated by organic compounds. These studies focus in the searching of new solutions in order to control the damages caused by the pollution from anthropogenic activities. In this context, the present study focused on the evaluation of different factors in the detoxification of the polycyclic aromatic hydrocarbon pyrene by marine-derived microbial consortium, applying the Design of Experiments (DOE) technique. Filamentous fungi Marasmiellus sp. CBMAI 1062 and Mucor racemosus CBMAI 847 from marine origin along with the bacteria Dietzia sp. CBMAI 705 and Bacillus sp. CBMAI 707 isolated from degraded petroleum provided efficient results in the toxicity tests, allowing a survival rate of up to 68.53% to the microcrustaceans Artemia sp. The low values obtained in the enzymatic activity evaluation indicate that lignin-degrading enzymes do not seem to be directly involved in the process of detoxification of pyrene by the studied consortium, suggesting that other enzymes or metabolic routes may have been participating in the detoxification process. Results from the present study highlight the biotechnological potential of the marine-derived microorganisms