Biological effects of soft denture reline materials on L929 cells in vitro
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://tej.sagepub.com/content/5/2041731414540911 http://hdl.handle.net/11449/125822 |
Resumo: | Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α5β1 and transforming growth factor β1 was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α5β1 and transforming growth factor β1. Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses. |
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Biological effects of soft denture reline materials on L929 cells in vitroSoft denture reline materialBiocompatibilityFibroblastIntegrin α5β1Transforming growth factor β1Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α5β1 and transforming growth factor β1 was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α5β1 and transforming growth factor β1. Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)London's Global University, Department of Biomaterials and Tissue Engineering, UCL Eastman Dental InstitutePlymouth University, Peninsula Schools of Medicine and DentistryUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Materiais Odontológicos e Prótese, Faculdade de Odontologia de AraraquaraUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Psicologia e Patologia, Faculdade de Odontologia de AraraquaraFAPESP: 2010/08750-0FAPESP: 2010/11764-3FAPESP: 2012/07994-9CNPq: 302543/2010-4Universidade Estadual Paulista (Unesp)London's Global UniversityPlymouth UniversityChaves, Carolina de Andrade Lima [UNESP]Vergani, Carlos Eduardo [UNESP]Thomas, DominiqueYoung, AnneCosta, Carlos Alberto de Souza [UNESP]Salih, Vehid MaxMachado, Ana Lucia [UNESP]2015-08-06T16:13:12Z2015-08-06T16:13:12Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://tej.sagepub.com/content/5/2041731414540911Journal of Tissue Engineering, v. 5, 2014.2041-7314http://hdl.handle.net/11449/12582210.1177/2041731414540911ISSN2041-7314-2014-05.pdf8000248781842587300313052242782045174842415155480000-0002-7375-4714Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Tissue Engineering5.789info:eu-repo/semantics/openAccess2023-11-28T06:12:56Zoai:repositorio.unesp.br:11449/125822Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-28T06:12:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological effects of soft denture reline materials on L929 cells in vitro |
title |
Biological effects of soft denture reline materials on L929 cells in vitro |
spellingShingle |
Biological effects of soft denture reline materials on L929 cells in vitro Chaves, Carolina de Andrade Lima [UNESP] Soft denture reline material Biocompatibility Fibroblast Integrin α5β1 Transforming growth factor β1 |
title_short |
Biological effects of soft denture reline materials on L929 cells in vitro |
title_full |
Biological effects of soft denture reline materials on L929 cells in vitro |
title_fullStr |
Biological effects of soft denture reline materials on L929 cells in vitro |
title_full_unstemmed |
Biological effects of soft denture reline materials on L929 cells in vitro |
title_sort |
Biological effects of soft denture reline materials on L929 cells in vitro |
author |
Chaves, Carolina de Andrade Lima [UNESP] |
author_facet |
Chaves, Carolina de Andrade Lima [UNESP] Vergani, Carlos Eduardo [UNESP] Thomas, Dominique Young, Anne Costa, Carlos Alberto de Souza [UNESP] Salih, Vehid Max Machado, Ana Lucia [UNESP] |
author_role |
author |
author2 |
Vergani, Carlos Eduardo [UNESP] Thomas, Dominique Young, Anne Costa, Carlos Alberto de Souza [UNESP] Salih, Vehid Max Machado, Ana Lucia [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) London's Global University Plymouth University |
dc.contributor.author.fl_str_mv |
Chaves, Carolina de Andrade Lima [UNESP] Vergani, Carlos Eduardo [UNESP] Thomas, Dominique Young, Anne Costa, Carlos Alberto de Souza [UNESP] Salih, Vehid Max Machado, Ana Lucia [UNESP] |
dc.subject.por.fl_str_mv |
Soft denture reline material Biocompatibility Fibroblast Integrin α5β1 Transforming growth factor β1 |
topic |
Soft denture reline material Biocompatibility Fibroblast Integrin α5β1 Transforming growth factor β1 |
description |
Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α5β1 and transforming growth factor β1 was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α5β1 and transforming growth factor β1. Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-08-06T16:13:12Z 2015-08-06T16:13:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://tej.sagepub.com/content/5/2041731414540911 Journal of Tissue Engineering, v. 5, 2014. 2041-7314 http://hdl.handle.net/11449/125822 10.1177/2041731414540911 ISSN2041-7314-2014-05.pdf 8000248781842587 3003130522427820 4517484241515548 0000-0002-7375-4714 |
url |
http://tej.sagepub.com/content/5/2041731414540911 http://hdl.handle.net/11449/125822 |
identifier_str_mv |
Journal of Tissue Engineering, v. 5, 2014. 2041-7314 10.1177/2041731414540911 ISSN2041-7314-2014-05.pdf 8000248781842587 3003130522427820 4517484241515548 0000-0002-7375-4714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Tissue Engineering 5.789 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965096367095808 |