Oxytocin Sustained Release Using Natural Rubber Latex Membranes

Detalhes bibliográficos
Autor(a) principal: Barros, Natan Roberto de [UNESP]
Data de Publicação: 2016
Outros Autores: Romeiro Miranda, Matheus Carlos [UNESP], Borges, Felipe Azevedo [UNESP], Mendonca, Ricardo Jose de, Cilli, Eduardo Maffud [UNESP], Herculano, Rondinelli Donizetti [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10989-016-9523-y
http://hdl.handle.net/11449/165352
Resumo: The demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest.
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spelling Oxytocin Sustained Release Using Natural Rubber Latex MembranesOxytocinNatural rubber latexPeptideSustained releaseBiomaterialThe demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Chem, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP, BrazilUFTM ICBN, Biochem Sect, 330 Manoel Mendes Sq, BR-38015050 Uberaba, MG, BrazilSao Paulo State Univ, Fac Pharmaceut Sci, Km01 Araraquara Jau Rd, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ, Inst Chem, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP, BrazilSao Paulo State Univ, Fac Pharmaceut Sci, Km01 Araraquara Jau Rd, BR-14801902 Araraquara, SP, BrazilFAPESP: 2014/17526-8FAPESP: 2015/02343-8SpringerUniversidade Estadual Paulista (Unesp)UFTM ICBNBarros, Natan Roberto de [UNESP]Romeiro Miranda, Matheus Carlos [UNESP]Borges, Felipe Azevedo [UNESP]Mendonca, Ricardo Jose deCilli, Eduardo Maffud [UNESP]Herculano, Rondinelli Donizetti [UNESP]2018-11-27T22:55:14Z2018-11-27T22:55:14Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article435-444application/pdfhttp://dx.doi.org/10.1007/s10989-016-9523-yInternational Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016.1573-3149http://hdl.handle.net/11449/16535210.1007/s10989-016-9523-yWOS:000386076400001WOS000386076400001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Peptide Research And Therapeutics0,333info:eu-repo/semantics/openAccess2024-06-13T17:38:20Zoai:repositorio.unesp.br:11449/165352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:28:52.726176Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Oxytocin Sustained Release Using Natural Rubber Latex Membranes
title Oxytocin Sustained Release Using Natural Rubber Latex Membranes
spellingShingle Oxytocin Sustained Release Using Natural Rubber Latex Membranes
Barros, Natan Roberto de [UNESP]
Oxytocin
Natural rubber latex
Peptide
Sustained release
Biomaterial
title_short Oxytocin Sustained Release Using Natural Rubber Latex Membranes
title_full Oxytocin Sustained Release Using Natural Rubber Latex Membranes
title_fullStr Oxytocin Sustained Release Using Natural Rubber Latex Membranes
title_full_unstemmed Oxytocin Sustained Release Using Natural Rubber Latex Membranes
title_sort Oxytocin Sustained Release Using Natural Rubber Latex Membranes
author Barros, Natan Roberto de [UNESP]
author_facet Barros, Natan Roberto de [UNESP]
Romeiro Miranda, Matheus Carlos [UNESP]
Borges, Felipe Azevedo [UNESP]
Mendonca, Ricardo Jose de
Cilli, Eduardo Maffud [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
author_role author
author2 Romeiro Miranda, Matheus Carlos [UNESP]
Borges, Felipe Azevedo [UNESP]
Mendonca, Ricardo Jose de
Cilli, Eduardo Maffud [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UFTM ICBN
dc.contributor.author.fl_str_mv Barros, Natan Roberto de [UNESP]
Romeiro Miranda, Matheus Carlos [UNESP]
Borges, Felipe Azevedo [UNESP]
Mendonca, Ricardo Jose de
Cilli, Eduardo Maffud [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
dc.subject.por.fl_str_mv Oxytocin
Natural rubber latex
Peptide
Sustained release
Biomaterial
topic Oxytocin
Natural rubber latex
Peptide
Sustained release
Biomaterial
description The demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
2018-11-27T22:55:14Z
2018-11-27T22:55:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10989-016-9523-y
International Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016.
1573-3149
http://hdl.handle.net/11449/165352
10.1007/s10989-016-9523-y
WOS:000386076400001
WOS000386076400001.pdf
url http://dx.doi.org/10.1007/s10989-016-9523-y
http://hdl.handle.net/11449/165352
identifier_str_mv International Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016.
1573-3149
10.1007/s10989-016-9523-y
WOS:000386076400001
WOS000386076400001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal Of Peptide Research And Therapeutics
0,333
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 435-444
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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