Oxytocin Sustained Release Using Natural Rubber Latex Membranes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10989-016-9523-y http://hdl.handle.net/11449/165352 |
Resumo: | The demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest. |
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Oxytocin Sustained Release Using Natural Rubber Latex MembranesOxytocinNatural rubber latexPeptideSustained releaseBiomaterialThe demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Chem, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP, BrazilUFTM ICBN, Biochem Sect, 330 Manoel Mendes Sq, BR-38015050 Uberaba, MG, BrazilSao Paulo State Univ, Fac Pharmaceut Sci, Km01 Araraquara Jau Rd, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ, Inst Chem, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP, BrazilSao Paulo State Univ, Fac Pharmaceut Sci, Km01 Araraquara Jau Rd, BR-14801902 Araraquara, SP, BrazilFAPESP: 2014/17526-8FAPESP: 2015/02343-8SpringerUniversidade Estadual Paulista (Unesp)UFTM ICBNBarros, Natan Roberto de [UNESP]Romeiro Miranda, Matheus Carlos [UNESP]Borges, Felipe Azevedo [UNESP]Mendonca, Ricardo Jose deCilli, Eduardo Maffud [UNESP]Herculano, Rondinelli Donizetti [UNESP]2018-11-27T22:55:14Z2018-11-27T22:55:14Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article435-444application/pdfhttp://dx.doi.org/10.1007/s10989-016-9523-yInternational Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016.1573-3149http://hdl.handle.net/11449/16535210.1007/s10989-016-9523-yWOS:000386076400001WOS000386076400001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Peptide Research And Therapeutics0,333info:eu-repo/semantics/openAccess2024-06-13T17:38:20Zoai:repositorio.unesp.br:11449/165352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:28:52.726176Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
title |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
spellingShingle |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes Barros, Natan Roberto de [UNESP] Oxytocin Natural rubber latex Peptide Sustained release Biomaterial |
title_short |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
title_full |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
title_fullStr |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
title_full_unstemmed |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
title_sort |
Oxytocin Sustained Release Using Natural Rubber Latex Membranes |
author |
Barros, Natan Roberto de [UNESP] |
author_facet |
Barros, Natan Roberto de [UNESP] Romeiro Miranda, Matheus Carlos [UNESP] Borges, Felipe Azevedo [UNESP] Mendonca, Ricardo Jose de Cilli, Eduardo Maffud [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
author_role |
author |
author2 |
Romeiro Miranda, Matheus Carlos [UNESP] Borges, Felipe Azevedo [UNESP] Mendonca, Ricardo Jose de Cilli, Eduardo Maffud [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UFTM ICBN |
dc.contributor.author.fl_str_mv |
Barros, Natan Roberto de [UNESP] Romeiro Miranda, Matheus Carlos [UNESP] Borges, Felipe Azevedo [UNESP] Mendonca, Ricardo Jose de Cilli, Eduardo Maffud [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
dc.subject.por.fl_str_mv |
Oxytocin Natural rubber latex Peptide Sustained release Biomaterial |
topic |
Oxytocin Natural rubber latex Peptide Sustained release Biomaterial |
description |
The demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 mu g mL(-1) (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 mu g mL(-1) (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 2018-11-27T22:55:14Z 2018-11-27T22:55:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10989-016-9523-y International Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016. 1573-3149 http://hdl.handle.net/11449/165352 10.1007/s10989-016-9523-y WOS:000386076400001 WOS000386076400001.pdf |
url |
http://dx.doi.org/10.1007/s10989-016-9523-y http://hdl.handle.net/11449/165352 |
identifier_str_mv |
International Journal Of Peptide Research And Therapeutics. New York: Springer, v. 22, n. 4, p. 435-444, 2016. 1573-3149 10.1007/s10989-016-9523-y WOS:000386076400001 WOS000386076400001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Peptide Research And Therapeutics 0,333 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
435-444 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128817309417472 |