Central muscarinic and LPBN mechanisms on sodium intake
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.brainresbull.2018.10.011 http://hdl.handle.net/11449/189887 |
Resumo: | Central cholinergic activation stimulates water intake, but also NaCl intake when the inhibitory mechanisms are blocked with injections of moxonidine (α2 adrenergic/imidazoline agonist) into the lateral parabrachial nucleus (LPBN). In the present study, we investigated the involvement of central M1 and M2 muscarinic receptors on NaCl intake induced by pilocarpine (non-selective muscarinic agonist) intraperitoneally combined with moxonidine into the LPBN or by muscimol (GABAA agonist) into the LPBN. Male Holtzman rats with stainless steel cannulas implanted bilaterally in the LPBN and in the lateral ventricle were used. Pirenzepine (M1 muscarinic antagonist, 1 nmol/1 μl) or methoctramine (M2 muscarinic antagonist, 50 nmol/1 μL) injected intracerebroventricularly (i.c.v.) reduced 0.3 M NaCl and water intake in rats treated with pilocarpine (0.1 mg/100 g of body weight) injected intraperitoneally combined with moxonidine (0.5 nmol/0.2 μL) into the LPBN. In rats treated with muscimol (0.5 nmol/0.2 μL) into the LPBN, methoctramine i.c.v. also reduced 0.3 M NaCl and water intake, however, pirenzepine produced no effect. The results suggest that M1 and M2 muscarinic receptors activate central pathways involved in the control of water and sodium intake that are under the influence of the LPBN inhibitory mechanisms. |
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Central muscarinic and LPBN mechanisms on sodium intakeCholinergicMethoctramineNaCl intakeParabrachialPirenzepineSodium appetiteCentral cholinergic activation stimulates water intake, but also NaCl intake when the inhibitory mechanisms are blocked with injections of moxonidine (α2 adrenergic/imidazoline agonist) into the lateral parabrachial nucleus (LPBN). In the present study, we investigated the involvement of central M1 and M2 muscarinic receptors on NaCl intake induced by pilocarpine (non-selective muscarinic agonist) intraperitoneally combined with moxonidine into the LPBN or by muscimol (GABAA agonist) into the LPBN. Male Holtzman rats with stainless steel cannulas implanted bilaterally in the LPBN and in the lateral ventricle were used. Pirenzepine (M1 muscarinic antagonist, 1 nmol/1 μl) or methoctramine (M2 muscarinic antagonist, 50 nmol/1 μL) injected intracerebroventricularly (i.c.v.) reduced 0.3 M NaCl and water intake in rats treated with pilocarpine (0.1 mg/100 g of body weight) injected intraperitoneally combined with moxonidine (0.5 nmol/0.2 μL) into the LPBN. In rats treated with muscimol (0.5 nmol/0.2 μL) into the LPBN, methoctramine i.c.v. also reduced 0.3 M NaCl and water intake, however, pirenzepine produced no effect. The results suggest that M1 and M2 muscarinic receptors activate central pathways involved in the control of water and sodium intake that are under the influence of the LPBN inhibitory mechanisms.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiology and Pathology School of Dentistry São Paulo State University - UNESPDepartment of Physiology and Pathology School of Dentistry São Paulo State University - UNESPUniversidade Estadual Paulista (Unesp)Anesio, Augusto [UNESP]Barbosa, Silas Pereira [UNESP]De Luca, Laurival A. [UNESP]de Paula, Patrícia Maria [UNESP]Colombari, Débora S.A. [UNESP]Colombari, Eduardo [UNESP]Andrade, Carina A.F. [UNESP]Menani, José V. [UNESP]2019-10-06T16:55:28Z2019-10-06T16:55:28Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14-20http://dx.doi.org/10.1016/j.brainresbull.2018.10.011Brain Research Bulletin, v. 144, p. 14-20.1873-27470361-9230http://hdl.handle.net/11449/18988710.1016/j.brainresbull.2018.10.0112-s2.0-85056648862Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Research Bulletininfo:eu-repo/semantics/openAccess2021-10-22T21:54:13Zoai:repositorio.unesp.br:11449/189887Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:54:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Central muscarinic and LPBN mechanisms on sodium intake |
title |
Central muscarinic and LPBN mechanisms on sodium intake |
spellingShingle |
Central muscarinic and LPBN mechanisms on sodium intake Anesio, Augusto [UNESP] Cholinergic Methoctramine NaCl intake Parabrachial Pirenzepine Sodium appetite |
title_short |
Central muscarinic and LPBN mechanisms on sodium intake |
title_full |
Central muscarinic and LPBN mechanisms on sodium intake |
title_fullStr |
Central muscarinic and LPBN mechanisms on sodium intake |
title_full_unstemmed |
Central muscarinic and LPBN mechanisms on sodium intake |
title_sort |
Central muscarinic and LPBN mechanisms on sodium intake |
author |
Anesio, Augusto [UNESP] |
author_facet |
Anesio, Augusto [UNESP] Barbosa, Silas Pereira [UNESP] De Luca, Laurival A. [UNESP] de Paula, Patrícia Maria [UNESP] Colombari, Débora S.A. [UNESP] Colombari, Eduardo [UNESP] Andrade, Carina A.F. [UNESP] Menani, José V. [UNESP] |
author_role |
author |
author2 |
Barbosa, Silas Pereira [UNESP] De Luca, Laurival A. [UNESP] de Paula, Patrícia Maria [UNESP] Colombari, Débora S.A. [UNESP] Colombari, Eduardo [UNESP] Andrade, Carina A.F. [UNESP] Menani, José V. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Anesio, Augusto [UNESP] Barbosa, Silas Pereira [UNESP] De Luca, Laurival A. [UNESP] de Paula, Patrícia Maria [UNESP] Colombari, Débora S.A. [UNESP] Colombari, Eduardo [UNESP] Andrade, Carina A.F. [UNESP] Menani, José V. [UNESP] |
dc.subject.por.fl_str_mv |
Cholinergic Methoctramine NaCl intake Parabrachial Pirenzepine Sodium appetite |
topic |
Cholinergic Methoctramine NaCl intake Parabrachial Pirenzepine Sodium appetite |
description |
Central cholinergic activation stimulates water intake, but also NaCl intake when the inhibitory mechanisms are blocked with injections of moxonidine (α2 adrenergic/imidazoline agonist) into the lateral parabrachial nucleus (LPBN). In the present study, we investigated the involvement of central M1 and M2 muscarinic receptors on NaCl intake induced by pilocarpine (non-selective muscarinic agonist) intraperitoneally combined with moxonidine into the LPBN or by muscimol (GABAA agonist) into the LPBN. Male Holtzman rats with stainless steel cannulas implanted bilaterally in the LPBN and in the lateral ventricle were used. Pirenzepine (M1 muscarinic antagonist, 1 nmol/1 μl) or methoctramine (M2 muscarinic antagonist, 50 nmol/1 μL) injected intracerebroventricularly (i.c.v.) reduced 0.3 M NaCl and water intake in rats treated with pilocarpine (0.1 mg/100 g of body weight) injected intraperitoneally combined with moxonidine (0.5 nmol/0.2 μL) into the LPBN. In rats treated with muscimol (0.5 nmol/0.2 μL) into the LPBN, methoctramine i.c.v. also reduced 0.3 M NaCl and water intake, however, pirenzepine produced no effect. The results suggest that M1 and M2 muscarinic receptors activate central pathways involved in the control of water and sodium intake that are under the influence of the LPBN inhibitory mechanisms. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:55:28Z 2019-10-06T16:55:28Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.brainresbull.2018.10.011 Brain Research Bulletin, v. 144, p. 14-20. 1873-2747 0361-9230 http://hdl.handle.net/11449/189887 10.1016/j.brainresbull.2018.10.011 2-s2.0-85056648862 |
url |
http://dx.doi.org/10.1016/j.brainresbull.2018.10.011 http://hdl.handle.net/11449/189887 |
identifier_str_mv |
Brain Research Bulletin, v. 144, p. 14-20. 1873-2747 0361-9230 10.1016/j.brainresbull.2018.10.011 2-s2.0-85056648862 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Research Bulletin |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14-20 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965704336703488 |