Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization

Detalhes bibliográficos
Autor(a) principal: dos Santos, Andréa G.
Data de Publicação: 2017
Outros Autores: Mendes, Érica A., de Oliveira, Rafael P., Faria, Ana M.C., de Sousa, Aurizangela O., Pirovani, Carlos P., de Araújo, Fernanda F., de Carvalho, Andréa T., Costa, Marliete Carvalho, Santos, Daniel Assis, Montoya, Quimi V. [UNESP], Rodrigues, Andre [UNESP], dos Santos, Jane L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2017.01681
http://hdl.handle.net/11449/220919
Resumo: The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis.
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spelling Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 PolarizationBiocontrol agentCandida parapsilosisConidiaPhagocytosisPRRsThe intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos Universidade Estadual de Santa CruzDepartamento de Microbiologia Instituto de Ciências Biológicas Universidade de São PauloInstituto Federal do ParanáDepartamento de Microbiologia Instituto de Ciências Biológicas Universidade Federal de Belo HorizonteDepartamento de Ciências Biológicas Universidade Estadual de Santa CruzGrupo Integrado de Pesquisas em Biomarcadores Centro de Pesquisas René Rachou Fundação Oswaldo CruzPrograma de Pós-Graduaçao em Sanidade e Producao Animal nos Trópicos Universidade de UberabaDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual de São PauloDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual de São PauloUniversidade Estadual de Santa CruzUniversidade de São Paulo (USP)Instituto Federal do ParanáUniversidade Federal de Belo HorizonteFundação Oswaldo CruzUniversidade de UberabaUniversidade Estadual Paulista (UNESP)dos Santos, Andréa G.Mendes, Érica A.de Oliveira, Rafael P.Faria, Ana M.C.de Sousa, Aurizangela O.Pirovani, Carlos P.de Araújo, Fernanda F.de Carvalho, Andréa T.Costa, Marliete CarvalhoSantos, Daniel AssisMontoya, Quimi V. [UNESP]Rodrigues, Andre [UNESP]dos Santos, Jane L.2022-04-28T19:06:57Z2022-04-28T19:06:57Z2017-09-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2017.01681Frontiers in Microbiology, v. 8, n. SEP, 2017.1664-302Xhttp://hdl.handle.net/11449/22091910.3389/fmicb.2017.016812-s2.0-85029232455Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T19:06:57Zoai:repositorio.unesp.br:11449/220919Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:01:25.758232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
title Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
spellingShingle Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
dos Santos, Andréa G.
Biocontrol agent
Candida parapsilosis
Conidia
Phagocytosis
PRRs
title_short Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
title_full Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
title_fullStr Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
title_full_unstemmed Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
title_sort Trichoderma asperelloides Spores Downregulate dectin1/2 and TLR2 Receptors of Mice Macrophages and Decrease Candida parapsilosis Phagocytosis Independent of the M1/M2 Polarization
author dos Santos, Andréa G.
author_facet dos Santos, Andréa G.
Mendes, Érica A.
de Oliveira, Rafael P.
Faria, Ana M.C.
de Sousa, Aurizangela O.
Pirovani, Carlos P.
de Araújo, Fernanda F.
de Carvalho, Andréa T.
Costa, Marliete Carvalho
Santos, Daniel Assis
Montoya, Quimi V. [UNESP]
Rodrigues, Andre [UNESP]
dos Santos, Jane L.
author_role author
author2 Mendes, Érica A.
de Oliveira, Rafael P.
Faria, Ana M.C.
de Sousa, Aurizangela O.
Pirovani, Carlos P.
de Araújo, Fernanda F.
de Carvalho, Andréa T.
Costa, Marliete Carvalho
Santos, Daniel Assis
Montoya, Quimi V. [UNESP]
Rodrigues, Andre [UNESP]
dos Santos, Jane L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Santa Cruz
Universidade de São Paulo (USP)
Instituto Federal do Paraná
Universidade Federal de Belo Horizonte
Fundação Oswaldo Cruz
Universidade de Uberaba
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv dos Santos, Andréa G.
Mendes, Érica A.
de Oliveira, Rafael P.
Faria, Ana M.C.
de Sousa, Aurizangela O.
Pirovani, Carlos P.
de Araújo, Fernanda F.
de Carvalho, Andréa T.
Costa, Marliete Carvalho
Santos, Daniel Assis
Montoya, Quimi V. [UNESP]
Rodrigues, Andre [UNESP]
dos Santos, Jane L.
dc.subject.por.fl_str_mv Biocontrol agent
Candida parapsilosis
Conidia
Phagocytosis
PRRs
topic Biocontrol agent
Candida parapsilosis
Conidia
Phagocytosis
PRRs
description The intensive use of pesticides to control pests in agriculture has promoted several issues relating to environment. As chemical pesticides remain controversial, biocontrol agents originating from fungi could be an alternative. Among them, we highlight biocontrol agents derived from the fungi genus Trichoderma, which have been documented in limiting the growth of other phytopathogenic fungus in the roots and leaves of several plant species. An important member of this genus is Trichoderma asperelloides, whose biocontrol agents have been used to promote plant growth while also treating soil diseases caused by microorganisms in both greenhouses and outdoor crops. To evaluate the safety of fungal biological agents for human health, tests to detect potentially adverse effects, such as allergenicity, toxicity, infectivity and pathogenicity, are crucial. In addition, identifying possible immunomodulating properties of fungal biocontrol agents merits further investigation. Thus, the aim of this study was to evaluate the effects of T. asperelloides spores in the internalization of Candida parapsilosis yeast by mice phagocytes, in order to elucidate the cellular and molecular mechanism of this interaction, as a model to understand possible in vivo effects of this fungus. For this, mice were exposed to a fungal spore suspension through-intraperitoneal injection, euthanized and cells from the peripheral blood and peritoneal cavity were collected for functional, quantitative and phenotypic analysis, throughout analysis of membrane receptors gene expression, phagocytosis ability and cells immunophenotyping M1 (CCR7 and CD86) and M2 (CCR2 and CD206). Our analyses showed that phagocytes exposed to fungal spores had reduced phagocytic capacity, as well as a decrease in the quantity of neutrophils and monocytes in the peripheral blood and peritoneal cavity. Moreover, macrophages exposed to T. asperelloides spores did not display the phenotypic profile M1/M2, and had reduced expression of pattern recognition receptors, such as TLR2, dectin-1 and dectin-2, all involved in the first line of defense against clinically important yeasts. Our data could infer that T. asperelloides spores may confer susceptibility to infection by C. parapsilosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-07
2022-04-28T19:06:57Z
2022-04-28T19:06:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2017.01681
Frontiers in Microbiology, v. 8, n. SEP, 2017.
1664-302X
http://hdl.handle.net/11449/220919
10.3389/fmicb.2017.01681
2-s2.0-85029232455
url http://dx.doi.org/10.3389/fmicb.2017.01681
http://hdl.handle.net/11449/220919
identifier_str_mv Frontiers in Microbiology, v. 8, n. SEP, 2017.
1664-302X
10.3389/fmicb.2017.01681
2-s2.0-85029232455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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