Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii

Detalhes bibliográficos
Autor(a) principal: Almeida, Eduardo Jose
Data de Publicação: 2012
Outros Autores: Wickert, Ester, Santos, Jaime Maia [UNESP], Gerald Martins, Antonio Baldo [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-29452012000200027
http://hdl.handle.net/11449/2146
Resumo: This study aimed to characterize molecular of 13 accessions of Psidium spp. (Myrtaceae) that was been identified for the reaction to rootknot guava nematode. The DNA extraction of the samples was carried according to the protocol of Shillito & Saul (1988). The molecular markers type fAFLP, were obtained from fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems from Brasil Ltda.) and were tested 24 selectives combinations of primers, of which 18 showed amplification that produced 272 polymorphic markers. To the analysis of the markers were employed the softwares GeneScan (ABI Prism versao 1.0) and Genotyper (ABI Prism version 1.03), and the data collected were transformed into a binary matrix that was analyzed in the software PAUP (Phylogenetic Analysis Using Parcimony - version 3.01). Were calculated genetic distance index intra and interespecific between the genotipes. It was found that the AFLP markers were efficient in the discrimination between accessions, as well as in showing genetic similarity among accessions identified as resistant to the nematode Meloidogyne enterolobii, which could be discussed in the future.
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spelling Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobiiAnalysis of genetic variability of Psidium spp. (Myrtaceae) access evaluated for resistant to Meloidogyne enterolobiiDisease resistanceTropical fruitPsidium guajavaRoot-knot nematodesgenetic markerThis study aimed to characterize molecular of 13 accessions of Psidium spp. (Myrtaceae) that was been identified for the reaction to rootknot guava nematode. The DNA extraction of the samples was carried according to the protocol of Shillito & Saul (1988). The molecular markers type fAFLP, were obtained from fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems from Brasil Ltda.) and were tested 24 selectives combinations of primers, of which 18 showed amplification that produced 272 polymorphic markers. To the analysis of the markers were employed the softwares GeneScan (ABI Prism versao 1.0) and Genotyper (ABI Prism version 1.03), and the data collected were transformed into a binary matrix that was analyzed in the software PAUP (Phylogenetic Analysis Using Parcimony - version 3.01). Were calculated genetic distance index intra and interespecific between the genotipes. It was found that the AFLP markers were efficient in the discrimination between accessions, as well as in showing genetic similarity among accessions identified as resistant to the nematode Meloidogyne enterolobii, which could be discussed in the future.Universidade Federal de São Carlos (UFSCar), Dept Agroecol, Ctr Ciencias Agr, Araras, SP, BrazilUNESP, FCAV, Dept Prod Vegetal, Jaboticabal, SP, BrazilUNESP, FCAV, Dept Fitossanidade, Jaboticabal, SP, BrazilUNESP, FCAV, Dept Prod Vegetal, Jaboticabal, SP, BrazilUNESP, FCAV, Dept Fitossanidade, Jaboticabal, SP, BrazilSoc Brasileira FruticulturaUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Almeida, Eduardo JoseWickert, EsterSantos, Jaime Maia [UNESP]Gerald Martins, Antonio Baldo [UNESP]2014-05-20T13:14:48Z2014-05-20T13:14:48Z2012-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article532-539http://dx.doi.org/10.1590/S0100-29452012000200027Revista Brasileira de Fruticultura. Jaboticabal Sp: Soc Brasileira Fruticultura, v. 34, n. 2, p. 532-539, 2012.0100-2945http://hdl.handle.net/11449/2146WOS:000307622900026Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Fruticultura0.4750,410info:eu-repo/semantics/openAccess2024-06-07T13:56:01Zoai:repositorio.unesp.br:11449/2146Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T13:56:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
Analysis of genetic variability of Psidium spp. (Myrtaceae) access evaluated for resistant to Meloidogyne enterolobii
title Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
spellingShingle Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
Almeida, Eduardo Jose
Disease resistance
Tropical fruit
Psidium guajava
Root-knot nematodes
genetic marker
title_short Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
title_full Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
title_fullStr Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
title_full_unstemmed Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
title_sort Análise da variabilidade genética de acessos dePsidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii
author Almeida, Eduardo Jose
author_facet Almeida, Eduardo Jose
Wickert, Ester
Santos, Jaime Maia [UNESP]
Gerald Martins, Antonio Baldo [UNESP]
author_role author
author2 Wickert, Ester
Santos, Jaime Maia [UNESP]
Gerald Martins, Antonio Baldo [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Almeida, Eduardo Jose
Wickert, Ester
Santos, Jaime Maia [UNESP]
Gerald Martins, Antonio Baldo [UNESP]
dc.subject.por.fl_str_mv Disease resistance
Tropical fruit
Psidium guajava
Root-knot nematodes
genetic marker
topic Disease resistance
Tropical fruit
Psidium guajava
Root-knot nematodes
genetic marker
description This study aimed to characterize molecular of 13 accessions of Psidium spp. (Myrtaceae) that was been identified for the reaction to rootknot guava nematode. The DNA extraction of the samples was carried according to the protocol of Shillito & Saul (1988). The molecular markers type fAFLP, were obtained from fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems from Brasil Ltda.) and were tested 24 selectives combinations of primers, of which 18 showed amplification that produced 272 polymorphic markers. To the analysis of the markers were employed the softwares GeneScan (ABI Prism versao 1.0) and Genotyper (ABI Prism version 1.03), and the data collected were transformed into a binary matrix that was analyzed in the software PAUP (Phylogenetic Analysis Using Parcimony - version 3.01). Were calculated genetic distance index intra and interespecific between the genotipes. It was found that the AFLP markers were efficient in the discrimination between accessions, as well as in showing genetic similarity among accessions identified as resistant to the nematode Meloidogyne enterolobii, which could be discussed in the future.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
2014-05-20T13:14:48Z
2014-05-20T13:14:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-29452012000200027
Revista Brasileira de Fruticultura. Jaboticabal Sp: Soc Brasileira Fruticultura, v. 34, n. 2, p. 532-539, 2012.
0100-2945
http://hdl.handle.net/11449/2146
WOS:000307622900026
url http://dx.doi.org/10.1590/S0100-29452012000200027
http://hdl.handle.net/11449/2146
identifier_str_mv Revista Brasileira de Fruticultura. Jaboticabal Sp: Soc Brasileira Fruticultura, v. 34, n. 2, p. 532-539, 2012.
0100-2945
WOS:000307622900026
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Fruticultura
0.475
0,410
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 532-539
dc.publisher.none.fl_str_mv Soc Brasileira Fruticultura
publisher.none.fl_str_mv Soc Brasileira Fruticultura
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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