Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S1431927621000313 http://hdl.handle.net/11449/206235 |
Resumo: | Obesity is characterized by excess adipose tissue and chronic inflammation and promotes extensive changes that can compromise skeletal muscles' structural and functional integrity. Obesity can seriously impact the force transmission region between the muscle and the tendon, the myotendinous junction (MTJ). The present study aimed to investigate the plasticity of muscle fibers and MTJ regions in high-fat diet-induced obesity in rat tibialis anterior (TA) and soleus (SO) muscles. Wistar rats were divided into control and obese groups (induced by a high-fat diet). The samples of TA and SO muscles were prepared for histochemical and ultrastructural analysis (sarcomeres and MTJ projection). In the muscle fiber, similar adaptations were observed between the muscles of the smaller fiber (types I and IIa) in the obesity results. The MTJ region demonstrated different adaptations between the analyzed muscles. The TA-MTJ region has shorter ultrastructures, while in the SO-MTJ region, the ultrastructures were larger. We conclude that obesity induced by a high-fat diet promotes similar adaptation in the muscle fibers; however, in the MTJ region, the sarcoplasmatic projections and adjacent sarcomere demonstrate different adaptations according to distinct muscle phenotypes. |
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Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Ratsextracellular matrixmyotendinous junctionobesitysarcomeresupport collagen layerObesity is characterized by excess adipose tissue and chronic inflammation and promotes extensive changes that can compromise skeletal muscles' structural and functional integrity. Obesity can seriously impact the force transmission region between the muscle and the tendon, the myotendinous junction (MTJ). The present study aimed to investigate the plasticity of muscle fibers and MTJ regions in high-fat diet-induced obesity in rat tibialis anterior (TA) and soleus (SO) muscles. Wistar rats were divided into control and obese groups (induced by a high-fat diet). The samples of TA and SO muscles were prepared for histochemical and ultrastructural analysis (sarcomeres and MTJ projection). In the muscle fiber, similar adaptations were observed between the muscles of the smaller fiber (types I and IIa) in the obesity results. The MTJ region demonstrated different adaptations between the analyzed muscles. The TA-MTJ region has shorter ultrastructures, while in the SO-MTJ region, the ultrastructures were larger. We conclude that obesity induced by a high-fat diet promotes similar adaptation in the muscle fibers; however, in the MTJ region, the sarcoplasmatic projections and adjacent sarcomere demonstrate different adaptations according to distinct muscle phenotypes.Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University (UNESP)Department of Anatomy Institute of Biomedical Sciences -ICB III University of São Paulo (USP)Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Grillo, Bruna Aléxia Cristofoletti [UNESP]Rocha, Lara C. [UNESP]Martinez, Giovana Z. [UNESP]Pimentel Neto, Jurandyr [UNESP]Jacob, Carolina Dos Santos [UNESP]Watanabe, Ii-SeiCiena, Adriano P. [UNESP]2021-06-25T10:28:41Z2021-06-25T10:28:41Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S1431927621000313Microscopy and Microanalysis.1435-81151431-9276http://hdl.handle.net/11449/20623510.1017/S14319276210003132-s2.0-85104733630Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy and Microanalysisinfo:eu-repo/semantics/openAccess2021-10-23T00:57:20Zoai:repositorio.unesp.br:11449/206235Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:47:06.577270Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
title |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
spellingShingle |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats Grillo, Bruna Aléxia Cristofoletti [UNESP] extracellular matrix myotendinous junction obesity sarcomere support collagen layer |
title_short |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
title_full |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
title_fullStr |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
title_full_unstemmed |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
title_sort |
Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats |
author |
Grillo, Bruna Aléxia Cristofoletti [UNESP] |
author_facet |
Grillo, Bruna Aléxia Cristofoletti [UNESP] Rocha, Lara C. [UNESP] Martinez, Giovana Z. [UNESP] Pimentel Neto, Jurandyr [UNESP] Jacob, Carolina Dos Santos [UNESP] Watanabe, Ii-Sei Ciena, Adriano P. [UNESP] |
author_role |
author |
author2 |
Rocha, Lara C. [UNESP] Martinez, Giovana Z. [UNESP] Pimentel Neto, Jurandyr [UNESP] Jacob, Carolina Dos Santos [UNESP] Watanabe, Ii-Sei Ciena, Adriano P. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Grillo, Bruna Aléxia Cristofoletti [UNESP] Rocha, Lara C. [UNESP] Martinez, Giovana Z. [UNESP] Pimentel Neto, Jurandyr [UNESP] Jacob, Carolina Dos Santos [UNESP] Watanabe, Ii-Sei Ciena, Adriano P. [UNESP] |
dc.subject.por.fl_str_mv |
extracellular matrix myotendinous junction obesity sarcomere support collagen layer |
topic |
extracellular matrix myotendinous junction obesity sarcomere support collagen layer |
description |
Obesity is characterized by excess adipose tissue and chronic inflammation and promotes extensive changes that can compromise skeletal muscles' structural and functional integrity. Obesity can seriously impact the force transmission region between the muscle and the tendon, the myotendinous junction (MTJ). The present study aimed to investigate the plasticity of muscle fibers and MTJ regions in high-fat diet-induced obesity in rat tibialis anterior (TA) and soleus (SO) muscles. Wistar rats were divided into control and obese groups (induced by a high-fat diet). The samples of TA and SO muscles were prepared for histochemical and ultrastructural analysis (sarcomeres and MTJ projection). In the muscle fiber, similar adaptations were observed between the muscles of the smaller fiber (types I and IIa) in the obesity results. The MTJ region demonstrated different adaptations between the analyzed muscles. The TA-MTJ region has shorter ultrastructures, while in the SO-MTJ region, the ultrastructures were larger. We conclude that obesity induced by a high-fat diet promotes similar adaptation in the muscle fibers; however, in the MTJ region, the sarcoplasmatic projections and adjacent sarcomere demonstrate different adaptations according to distinct muscle phenotypes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:41Z 2021-06-25T10:28:41Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S1431927621000313 Microscopy and Microanalysis. 1435-8115 1431-9276 http://hdl.handle.net/11449/206235 10.1017/S1431927621000313 2-s2.0-85104733630 |
url |
http://dx.doi.org/10.1017/S1431927621000313 http://hdl.handle.net/11449/206235 |
identifier_str_mv |
Microscopy and Microanalysis. 1435-8115 1431-9276 10.1017/S1431927621000313 2-s2.0-85104733630 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy and Microanalysis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128416898088960 |