Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S1431927622000186 http://hdl.handle.net/11449/223678 |
Resumo: | The myotendinous junction (MTJ) is a highly specialized region of the locomotor apparatus. Here, we investigated the ultrastructural and molecular effects in the MTJ region after static stretching prior to the ladder-based resistance training. Thirty-two male, 60-day old Wistar rats were divided into four groups: Sedentary, Resistance Training, Stretching, and Stretching-Resistance Training. The gastrocnemius muscle was processed for transmission electron microscopy techniques and Western blot assay. We observed that the static stretching prior to the ladder-based resistance training increased the MTJ components, the fibroblast growth factor (FGF)-2 and FGF-6 protein expression. Also, we demonstrated the lower transforming growth factor expression and no difference in the lysyl oxidase expression after combined training. The MTJ alterations in response to combined training demonstrate adaptive mechanisms which can be used for the prescription or development of methods to reduce or prevent injuries in humans and promote the myotendinous interface benefit. |
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Repositório Institucional da UNESP |
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spelling |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance ExerciseFGFmyotendinous interfaceresistance trainingsarcomereTGF-β1The myotendinous junction (MTJ) is a highly specialized region of the locomotor apparatus. Here, we investigated the ultrastructural and molecular effects in the MTJ region after static stretching prior to the ladder-based resistance training. Thirty-two male, 60-day old Wistar rats were divided into four groups: Sedentary, Resistance Training, Stretching, and Stretching-Resistance Training. The gastrocnemius muscle was processed for transmission electron microscopy techniques and Western blot assay. We observed that the static stretching prior to the ladder-based resistance training increased the MTJ components, the fibroblast growth factor (FGF)-2 and FGF-6 protein expression. Also, we demonstrated the lower transforming growth factor expression and no difference in the lysyl oxidase expression after combined training. The MTJ alterations in response to combined training demonstrate adaptive mechanisms which can be used for the prescription or development of methods to reduce or prevent injuries in humans and promote the myotendinous interface benefit.Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences São Paulo State University (UNESP), SPLaboratory of Functional Neuroanatomy of Pain (LAND) Department of Anatomy Universidade de Sao Paulo (USP), SPLaboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Jacob, Carolina Dos S. [UNESP]Barbosa, Gabriela K. [UNESP]Rodrigues, Mariana P. [UNESP]Pimentel Neto, Jurandyr [UNESP]Rocha-Braga, Lara C. [UNESP]De Oliveira, Camilla G.Chacur, MaruciaCiena, Adriano P. [UNESP]2022-04-28T19:52:04Z2022-04-28T19:52:04Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S1431927622000186Microscopy and Microanalysis.1435-81151431-9276http://hdl.handle.net/11449/22367810.1017/S14319276220001862-s2.0-85126641627Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy and Microanalysisinfo:eu-repo/semantics/openAccess2022-04-28T19:52:05Zoai:repositorio.unesp.br:11449/223678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:45:49.598539Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
title |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
spellingShingle |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise Jacob, Carolina Dos S. [UNESP] FGF myotendinous interface resistance training sarcomere TGF-β1 |
title_short |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
title_full |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
title_fullStr |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
title_full_unstemmed |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
title_sort |
Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise |
author |
Jacob, Carolina Dos S. [UNESP] |
author_facet |
Jacob, Carolina Dos S. [UNESP] Barbosa, Gabriela K. [UNESP] Rodrigues, Mariana P. [UNESP] Pimentel Neto, Jurandyr [UNESP] Rocha-Braga, Lara C. [UNESP] De Oliveira, Camilla G. Chacur, Marucia Ciena, Adriano P. [UNESP] |
author_role |
author |
author2 |
Barbosa, Gabriela K. [UNESP] Rodrigues, Mariana P. [UNESP] Pimentel Neto, Jurandyr [UNESP] Rocha-Braga, Lara C. [UNESP] De Oliveira, Camilla G. Chacur, Marucia Ciena, Adriano P. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Jacob, Carolina Dos S. [UNESP] Barbosa, Gabriela K. [UNESP] Rodrigues, Mariana P. [UNESP] Pimentel Neto, Jurandyr [UNESP] Rocha-Braga, Lara C. [UNESP] De Oliveira, Camilla G. Chacur, Marucia Ciena, Adriano P. [UNESP] |
dc.subject.por.fl_str_mv |
FGF myotendinous interface resistance training sarcomere TGF-β1 |
topic |
FGF myotendinous interface resistance training sarcomere TGF-β1 |
description |
The myotendinous junction (MTJ) is a highly specialized region of the locomotor apparatus. Here, we investigated the ultrastructural and molecular effects in the MTJ region after static stretching prior to the ladder-based resistance training. Thirty-two male, 60-day old Wistar rats were divided into four groups: Sedentary, Resistance Training, Stretching, and Stretching-Resistance Training. The gastrocnemius muscle was processed for transmission electron microscopy techniques and Western blot assay. We observed that the static stretching prior to the ladder-based resistance training increased the MTJ components, the fibroblast growth factor (FGF)-2 and FGF-6 protein expression. Also, we demonstrated the lower transforming growth factor expression and no difference in the lysyl oxidase expression after combined training. The MTJ alterations in response to combined training demonstrate adaptive mechanisms which can be used for the prescription or development of methods to reduce or prevent injuries in humans and promote the myotendinous interface benefit. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:04Z 2022-04-28T19:52:04Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S1431927622000186 Microscopy and Microanalysis. 1435-8115 1431-9276 http://hdl.handle.net/11449/223678 10.1017/S1431927622000186 2-s2.0-85126641627 |
url |
http://dx.doi.org/10.1017/S1431927622000186 http://hdl.handle.net/11449/223678 |
identifier_str_mv |
Microscopy and Microanalysis. 1435-8115 1431-9276 10.1017/S1431927622000186 2-s2.0-85126641627 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy and Microanalysis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128854160572416 |