PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Rebech, Gabriela Torres [UNESP]
Data de Publicação: 2020
Outros Autores: Venturin, Gabriela Lovizutto [UNESP], Siqueira Ito, Lucas Takeshi [UNESP], Bragato, Jaqueline Poleto [UNESP], de Carvalho Fonseca, Bianca Stefanini [UNESP], Melo, Larrissa Martins [UNESP], Costa, Sidnei Ferro [UNESP], de Rezende Eugênio, Flávia [UNESP], dos Santos, Paulo Sérgio Patto [UNESP], de Lima, Valéria Marçal Felix [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetimm.2019.109970
http://hdl.handle.net/11449/198108
Resumo: Leishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future.
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spelling PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasisCD279CytokinesProtozoaSpleen leukocytesLeishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future.São Paulo State University (UNESP) School of Veterinary MedicineDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793São Paulo State University (UNESP) School of Veterinary MedicineDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793Universidade Estadual Paulista (Unesp)Rebech, Gabriela Torres [UNESP]Venturin, Gabriela Lovizutto [UNESP]Siqueira Ito, Lucas Takeshi [UNESP]Bragato, Jaqueline Poleto [UNESP]de Carvalho Fonseca, Bianca Stefanini [UNESP]Melo, Larrissa Martins [UNESP]Costa, Sidnei Ferro [UNESP]de Rezende Eugênio, Flávia [UNESP]dos Santos, Paulo Sérgio Patto [UNESP]de Lima, Valéria Marçal Felix [UNESP]2020-12-12T00:59:20Z2020-12-12T00:59:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetimm.2019.109970Veterinary Immunology and Immunopathology, v. 219.1873-25340165-2427http://hdl.handle.net/11449/19810810.1016/j.vetimm.2019.1099702-s2.0-85074711194Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/openAccess2021-10-23T08:46:54Zoai:repositorio.unesp.br:11449/198108Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:30.333203Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
title PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
spellingShingle PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
Rebech, Gabriela Torres [UNESP]
CD279
Cytokines
Protozoa
Spleen leukocytes
title_short PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
title_full PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
title_fullStr PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
title_full_unstemmed PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
title_sort PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
author Rebech, Gabriela Torres [UNESP]
author_facet Rebech, Gabriela Torres [UNESP]
Venturin, Gabriela Lovizutto [UNESP]
Siqueira Ito, Lucas Takeshi [UNESP]
Bragato, Jaqueline Poleto [UNESP]
de Carvalho Fonseca, Bianca Stefanini [UNESP]
Melo, Larrissa Martins [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flávia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author_role author
author2 Venturin, Gabriela Lovizutto [UNESP]
Siqueira Ito, Lucas Takeshi [UNESP]
Bragato, Jaqueline Poleto [UNESP]
de Carvalho Fonseca, Bianca Stefanini [UNESP]
Melo, Larrissa Martins [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flávia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rebech, Gabriela Torres [UNESP]
Venturin, Gabriela Lovizutto [UNESP]
Siqueira Ito, Lucas Takeshi [UNESP]
Bragato, Jaqueline Poleto [UNESP]
de Carvalho Fonseca, Bianca Stefanini [UNESP]
Melo, Larrissa Martins [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flávia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
dc.subject.por.fl_str_mv CD279
Cytokines
Protozoa
Spleen leukocytes
topic CD279
Cytokines
Protozoa
Spleen leukocytes
description Leishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T00:59:20Z
2020-12-12T00:59:20Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2019.109970
Veterinary Immunology and Immunopathology, v. 219.
1873-2534
0165-2427
http://hdl.handle.net/11449/198108
10.1016/j.vetimm.2019.109970
2-s2.0-85074711194
url http://dx.doi.org/10.1016/j.vetimm.2019.109970
http://hdl.handle.net/11449/198108
identifier_str_mv Veterinary Immunology and Immunopathology, v. 219.
1873-2534
0165-2427
10.1016/j.vetimm.2019.109970
2-s2.0-85074711194
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Immunology and Immunopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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