PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetimm.2019.109970 http://hdl.handle.net/11449/198108 |
Resumo: | Leishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future. |
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Repositório Institucional da UNESP |
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PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasisCD279CytokinesProtozoaSpleen leukocytesLeishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future.São Paulo State University (UNESP) School of Veterinary MedicineDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793São Paulo State University (UNESP) School of Veterinary MedicineDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, Rua Clovis Pestana, 793Universidade Estadual Paulista (Unesp)Rebech, Gabriela Torres [UNESP]Venturin, Gabriela Lovizutto [UNESP]Siqueira Ito, Lucas Takeshi [UNESP]Bragato, Jaqueline Poleto [UNESP]de Carvalho Fonseca, Bianca Stefanini [UNESP]Melo, Larrissa Martins [UNESP]Costa, Sidnei Ferro [UNESP]de Rezende Eugênio, Flávia [UNESP]dos Santos, Paulo Sérgio Patto [UNESP]de Lima, Valéria Marçal Felix [UNESP]2020-12-12T00:59:20Z2020-12-12T00:59:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetimm.2019.109970Veterinary Immunology and Immunopathology, v. 219.1873-25340165-2427http://hdl.handle.net/11449/19810810.1016/j.vetimm.2019.1099702-s2.0-85074711194Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/openAccess2021-10-23T08:46:54Zoai:repositorio.unesp.br:11449/198108Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:30.333203Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
title |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
spellingShingle |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis Rebech, Gabriela Torres [UNESP] CD279 Cytokines Protozoa Spleen leukocytes |
title_short |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
title_full |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
title_fullStr |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
title_full_unstemmed |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
title_sort |
PD-1 regulates leishmanicidal activity and IL-17 in dogs with leishmaniasis |
author |
Rebech, Gabriela Torres [UNESP] |
author_facet |
Rebech, Gabriela Torres [UNESP] Venturin, Gabriela Lovizutto [UNESP] Siqueira Ito, Lucas Takeshi [UNESP] Bragato, Jaqueline Poleto [UNESP] de Carvalho Fonseca, Bianca Stefanini [UNESP] Melo, Larrissa Martins [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flávia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author_role |
author |
author2 |
Venturin, Gabriela Lovizutto [UNESP] Siqueira Ito, Lucas Takeshi [UNESP] Bragato, Jaqueline Poleto [UNESP] de Carvalho Fonseca, Bianca Stefanini [UNESP] Melo, Larrissa Martins [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flávia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rebech, Gabriela Torres [UNESP] Venturin, Gabriela Lovizutto [UNESP] Siqueira Ito, Lucas Takeshi [UNESP] Bragato, Jaqueline Poleto [UNESP] de Carvalho Fonseca, Bianca Stefanini [UNESP] Melo, Larrissa Martins [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flávia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
dc.subject.por.fl_str_mv |
CD279 Cytokines Protozoa Spleen leukocytes |
topic |
CD279 Cytokines Protozoa Spleen leukocytes |
description |
Leishmaniasis is an immunosuppressive disease caused by protozoa of the genus Leishmania, for which dogs are the domestic reservoir. The programmed cell death-1 molecule (PD-1) is highly expressed in leukocyte cells of dogs with leishmaniasis, and it promotes T lymphocyte exhaustion and suppression of cytokine secretion. Because PD-1 has a suppressive function regarding cell immunity, we evaluated the effect of PD-1 blocking antibodies on NO, ROS and interleukin 17 (IL-17) production and on parasite load in spleen leukocyte cultures from dogs with leishmaniasis. In vitro, PD-1 blocking promoted increased levels of intracellular NO and NO2 and reduced the levels of IL-17 in the culture supernatant, in addition to reducing the parasite load, but it did not change ROS levels. We conclude that PD-1 participates in the regulation of the immune response and that the blocking antibody is effective in restoring host microbicidal activity. This can be investigated in an immunotherapeutic study in the future. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T00:59:20Z 2020-12-12T00:59:20Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetimm.2019.109970 Veterinary Immunology and Immunopathology, v. 219. 1873-2534 0165-2427 http://hdl.handle.net/11449/198108 10.1016/j.vetimm.2019.109970 2-s2.0-85074711194 |
url |
http://dx.doi.org/10.1016/j.vetimm.2019.109970 http://hdl.handle.net/11449/198108 |
identifier_str_mv |
Veterinary Immunology and Immunopathology, v. 219. 1873-2534 0165-2427 10.1016/j.vetimm.2019.109970 2-s2.0-85074711194 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Immunology and Immunopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129276638134272 |