Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study

Detalhes bibliográficos
Autor(a) principal: Cassiano, Luiza P.S.
Data de Publicação: 2018
Outros Autores: Ventura, Talita M.S., Silva, Cintia M.S., Leite, Aline L., Magalhães, Ana C., Pessan, Juliano P. [UNESP], Buzalaf, Marília Afonso Rabelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000485390
http://hdl.handle.net/11449/177089
Resumo: This study detected changes in the protein profile of the acquired enamel pellicle (AEP) formed in vivo after rinsing with whole milk, fat-free milk, or water. Nine subjects in good oral condition took part in the study. The acquired pellicle was formed in the morning, for 120 min, after prophylaxis with pumice. Following this, the volunteers rinsed with 10 mL of whole milk, fat-free milk, or deionized water for 30 s, following a blinded crossover protocol. After 60 min, the pellicle was collected with filter paper soaked in 3% citric acid and processed for analysis by liquid chromatography-electrospray ionization tandem mass spectrometry. The obtained tandem mass spectrometry spectra were searched against a human protein database (Swiss-Prot). The proteomic data related to protein quantification were analysed using the PLGS software. A total of 260 proteins were successfully identified in the AEP samples collected from all groups. Forty-nine were common to all 3 groups, while 72, 62, and 49 were specific to the groups rinsing with whole milk, fat-free milk, and water, respectively. Some were typical components of the AEP, such as cystatin-B, cystatin-SN, isoforms of α-amylase, IgA and IgG, lysozyme C, protein S100 A78, histatin-1, proline-rich protein 27, statherin, and lactotransferrin. Other proteins are not commonly described as part of the AEP but could act in defence of the organism against pathogens. Distinct proteomic profiles were found in the AEP after rinsing with whole or fat-free milk, which could have an impact on bacterial adhesion and tooth dissolution. The use of fat-free milk could favourably modulate the adhesion of bacteria to the AEP as well as biofilm formation when compared with whole milk.
id UNSP_78de6fc6736687f4bb163cabae34f947
oai_identifier_str oai:repositorio.unesp.br:11449/177089
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo StudyAcquired pellicleCariesEnamelErosionMilkProteomicsThis study detected changes in the protein profile of the acquired enamel pellicle (AEP) formed in vivo after rinsing with whole milk, fat-free milk, or water. Nine subjects in good oral condition took part in the study. The acquired pellicle was formed in the morning, for 120 min, after prophylaxis with pumice. Following this, the volunteers rinsed with 10 mL of whole milk, fat-free milk, or deionized water for 30 s, following a blinded crossover protocol. After 60 min, the pellicle was collected with filter paper soaked in 3% citric acid and processed for analysis by liquid chromatography-electrospray ionization tandem mass spectrometry. The obtained tandem mass spectrometry spectra were searched against a human protein database (Swiss-Prot). The proteomic data related to protein quantification were analysed using the PLGS software. A total of 260 proteins were successfully identified in the AEP samples collected from all groups. Forty-nine were common to all 3 groups, while 72, 62, and 49 were specific to the groups rinsing with whole milk, fat-free milk, and water, respectively. Some were typical components of the AEP, such as cystatin-B, cystatin-SN, isoforms of α-amylase, IgA and IgG, lysozyme C, protein S100 A78, histatin-1, proline-rich protein 27, statherin, and lactotransferrin. Other proteins are not commonly described as part of the AEP but could act in defence of the organism against pathogens. Distinct proteomic profiles were found in the AEP after rinsing with whole or fat-free milk, which could have an impact on bacterial adhesion and tooth dissolution. The use of fat-free milk could favourably modulate the adhesion of bacteria to the AEP as well as biofilm formation when compared with whole milk.Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75Araçatuba Dental School Universidade Estadual Paulista (UNESP)Araçatuba Dental School Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Cassiano, Luiza P.S.Ventura, Talita M.S.Silva, Cintia M.S.Leite, Aline L.Magalhães, Ana C.Pessan, Juliano P. [UNESP]Buzalaf, Marília Afonso Rabelo2018-12-11T17:23:57Z2018-12-11T17:23:57Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article288-296application/pdfhttp://dx.doi.org/10.1159/000485390Caries Research, v. 52, n. 4, p. 288-296, 2018.1421-976X0008-6568http://hdl.handle.net/11449/17708910.1159/0004853902-s2.0-850415715812-s2.0-85041571581.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCaries Research1,108info:eu-repo/semantics/openAccess2024-04-23T15:23:50Zoai:repositorio.unesp.br:11449/177089Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:45:16.156700Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
title Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
spellingShingle Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
Cassiano, Luiza P.S.
Acquired pellicle
Caries
Enamel
Erosion
Milk
Proteomics
title_short Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
title_full Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
title_fullStr Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
title_full_unstemmed Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
title_sort Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study
author Cassiano, Luiza P.S.
author_facet Cassiano, Luiza P.S.
Ventura, Talita M.S.
Silva, Cintia M.S.
Leite, Aline L.
Magalhães, Ana C.
Pessan, Juliano P. [UNESP]
Buzalaf, Marília Afonso Rabelo
author_role author
author2 Ventura, Talita M.S.
Silva, Cintia M.S.
Leite, Aline L.
Magalhães, Ana C.
Pessan, Juliano P. [UNESP]
Buzalaf, Marília Afonso Rabelo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cassiano, Luiza P.S.
Ventura, Talita M.S.
Silva, Cintia M.S.
Leite, Aline L.
Magalhães, Ana C.
Pessan, Juliano P. [UNESP]
Buzalaf, Marília Afonso Rabelo
dc.subject.por.fl_str_mv Acquired pellicle
Caries
Enamel
Erosion
Milk
Proteomics
topic Acquired pellicle
Caries
Enamel
Erosion
Milk
Proteomics
description This study detected changes in the protein profile of the acquired enamel pellicle (AEP) formed in vivo after rinsing with whole milk, fat-free milk, or water. Nine subjects in good oral condition took part in the study. The acquired pellicle was formed in the morning, for 120 min, after prophylaxis with pumice. Following this, the volunteers rinsed with 10 mL of whole milk, fat-free milk, or deionized water for 30 s, following a blinded crossover protocol. After 60 min, the pellicle was collected with filter paper soaked in 3% citric acid and processed for analysis by liquid chromatography-electrospray ionization tandem mass spectrometry. The obtained tandem mass spectrometry spectra were searched against a human protein database (Swiss-Prot). The proteomic data related to protein quantification were analysed using the PLGS software. A total of 260 proteins were successfully identified in the AEP samples collected from all groups. Forty-nine were common to all 3 groups, while 72, 62, and 49 were specific to the groups rinsing with whole milk, fat-free milk, and water, respectively. Some were typical components of the AEP, such as cystatin-B, cystatin-SN, isoforms of α-amylase, IgA and IgG, lysozyme C, protein S100 A78, histatin-1, proline-rich protein 27, statherin, and lactotransferrin. Other proteins are not commonly described as part of the AEP but could act in defence of the organism against pathogens. Distinct proteomic profiles were found in the AEP after rinsing with whole or fat-free milk, which could have an impact on bacterial adhesion and tooth dissolution. The use of fat-free milk could favourably modulate the adhesion of bacteria to the AEP as well as biofilm formation when compared with whole milk.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:23:57Z
2018-12-11T17:23:57Z
2018-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000485390
Caries Research, v. 52, n. 4, p. 288-296, 2018.
1421-976X
0008-6568
http://hdl.handle.net/11449/177089
10.1159/000485390
2-s2.0-85041571581
2-s2.0-85041571581.pdf
url http://dx.doi.org/10.1159/000485390
http://hdl.handle.net/11449/177089
identifier_str_mv Caries Research, v. 52, n. 4, p. 288-296, 2018.
1421-976X
0008-6568
10.1159/000485390
2-s2.0-85041571581
2-s2.0-85041571581.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Caries Research
1,108
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 288-296
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129354569351168