Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822011000300023 http://hdl.handle.net/11449/26645 |
Resumo: | The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly polymorphic and distantly genetically related taxa (A and B) were identified within the total population of yeasts, each contained subgroups (A1, A2, A3, A4, B1 and B2) and clusters of moderately related strains (from I to X), suggesting the existence of strains restricted or not to certain groups of geographically limited, healthy students. However, the coexistence of identical strains in healthy schoolchildren from the same school (geographically related) reinforces the hypothesis of oral transmission, where the sources of propagation could be explored. Furthermore, this could also be used in current and retrospective analyses of C. albicans isolated from immunocompetent and immunocompromised people, in order to detect commensal or potentially pathogenic yeast groups, predominantly in candidiasis, and in the development of strategies to prevent transmission or human propagation. |
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Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxaCandida albicanshealthy schoolchildrengeographic regionMLEEcluster analysisThe genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly polymorphic and distantly genetically related taxa (A and B) were identified within the total population of yeasts, each contained subgroups (A1, A2, A3, A4, B1 and B2) and clusters of moderately related strains (from I to X), suggesting the existence of strains restricted or not to certain groups of geographically limited, healthy students. However, the coexistence of identical strains in healthy schoolchildren from the same school (geographically related) reinforces the hypothesis of oral transmission, where the sources of propagation could be explored. Furthermore, this could also be used in current and retrospective analyses of C. albicans isolated from immunocompetent and immunocompromised people, in order to detect commensal or potentially pathogenic yeast groups, predominantly in candidiasis, and in the development of strategies to prevent transmission or human propagation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de Alfenas Faculdade de Ciências Médicas Laboratório de Genética e Biologia MolecularUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Patologia and Fisiologia OralPontificia Universidade Católica do Paraná Centro de Ciências Biológicas e da Saúde Laboratório de Microbiologia OralUniversidade Estadual de Campinas Faculdade de Odontologia Laboratório de Microbiologia Oral e ImunologiaUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Patologia and Fisiologia OralSociedade Brasileira de MicrobiologiaUniversidade de Alfenas Faculdade de Ciências Médicas Laboratório de Genética e Biologia MolecularUniversidade Estadual Paulista (Unesp)Pontifícia Universidade Católica do Paraná (PUCPR)Universidade Estadual de Campinas (UNICAMP)Boriollo, Marcelo Fabiano GomesSpolidório, Denise Madalena Palomari [UNESP]Barros, Letizia MonteiroBassi, Rodrigo CarlosGarcia, José Antonio DiasCosta, Ana Maria Duarte DiasRosa, Edvaldo Antonio RibeiroHöfling, José Francisco2014-05-20T15:07:44Z2014-05-20T15:07:44Z2011-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1030-1046application/pdfhttp://dx.doi.org/10.1590/S1517-83822011000300023Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 3, p. 1030-1046, 2011.1517-8382http://hdl.handle.net/11449/2664510.1590/S1517-83822011000300023S1517-83822011000300023WOS:000297756800023S1517-83822011000300023.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2023-10-11T06:03:06Zoai:repositorio.unesp.br:11449/26645Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-11T06:03:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
title |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
spellingShingle |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa Boriollo, Marcelo Fabiano Gomes Candida albicans healthy schoolchildren geographic region MLEE cluster analysis |
title_short |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
title_full |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
title_fullStr |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
title_full_unstemmed |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
title_sort |
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa |
author |
Boriollo, Marcelo Fabiano Gomes |
author_facet |
Boriollo, Marcelo Fabiano Gomes Spolidório, Denise Madalena Palomari [UNESP] Barros, Letizia Monteiro Bassi, Rodrigo Carlos Garcia, José Antonio Dias Costa, Ana Maria Duarte Dias Rosa, Edvaldo Antonio Ribeiro Höfling, José Francisco |
author_role |
author |
author2 |
Spolidório, Denise Madalena Palomari [UNESP] Barros, Letizia Monteiro Bassi, Rodrigo Carlos Garcia, José Antonio Dias Costa, Ana Maria Duarte Dias Rosa, Edvaldo Antonio Ribeiro Höfling, José Francisco |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Alfenas Faculdade de Ciências Médicas Laboratório de Genética e Biologia Molecular Universidade Estadual Paulista (Unesp) Pontifícia Universidade Católica do Paraná (PUCPR) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Boriollo, Marcelo Fabiano Gomes Spolidório, Denise Madalena Palomari [UNESP] Barros, Letizia Monteiro Bassi, Rodrigo Carlos Garcia, José Antonio Dias Costa, Ana Maria Duarte Dias Rosa, Edvaldo Antonio Ribeiro Höfling, José Francisco |
dc.subject.por.fl_str_mv |
Candida albicans healthy schoolchildren geographic region MLEE cluster analysis |
topic |
Candida albicans healthy schoolchildren geographic region MLEE cluster analysis |
description |
The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly polymorphic and distantly genetically related taxa (A and B) were identified within the total population of yeasts, each contained subgroups (A1, A2, A3, A4, B1 and B2) and clusters of moderately related strains (from I to X), suggesting the existence of strains restricted or not to certain groups of geographically limited, healthy students. However, the coexistence of identical strains in healthy schoolchildren from the same school (geographically related) reinforces the hypothesis of oral transmission, where the sources of propagation could be explored. Furthermore, this could also be used in current and retrospective analyses of C. albicans isolated from immunocompetent and immunocompromised people, in order to detect commensal or potentially pathogenic yeast groups, predominantly in candidiasis, and in the development of strategies to prevent transmission or human propagation. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 2014-05-20T15:07:44Z 2014-05-20T15:07:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822011000300023 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 3, p. 1030-1046, 2011. 1517-8382 http://hdl.handle.net/11449/26645 10.1590/S1517-83822011000300023 S1517-83822011000300023 WOS:000297756800023 S1517-83822011000300023.pdf |
url |
http://dx.doi.org/10.1590/S1517-83822011000300023 http://hdl.handle.net/11449/26645 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 3, p. 1030-1046, 2011. 1517-8382 10.1590/S1517-83822011000300023 S1517-83822011000300023 WOS:000297756800023 S1517-83822011000300023.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1030-1046 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045932182274048 |