From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass

Detalhes bibliográficos
Autor(a) principal: Micheletti, Chiara
Data de Publicação: 2021
Outros Autores: Gomes-Ferreira, Pedro Henrique Silva [UNESP], Casagrande, Travis, Lisboa-Filho, Paulo Noronha [UNESP], Okamoto, Roberta [UNESP], Grandfield, Kathryn
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1098/rsif.2021.0181
http://hdl.handle.net/11449/229612
Resumo: The success of biomaterials for bone regeneration relies on many factors, among which osseointegration plays a key role. Biogran (BG) is a bioactive glass commonly employed as a bone graft in dental procedures. Despite its use in clinical practice, the capability of BG to promote osseointegration has never been resolved at the nanoscale. In this paper, we present the workflow for characterizing the interface between newly formed bone and BG in a preclinical rat model. Areas of bone-BG contact were first identified by backscattered electron imaging in a scanning electron microscope. A focused ion beam in situ lift-out protocol was employed to prepare ultrathin samples for transmission electron microscopy analysis. The bone-BG gradual interface, i.e. the biointerphase, was visualized at the nanoscale with unprecedented resolution thanks to scanning transmission electron microscopy. Finally, we present a method to view the bone-BG interface in three dimensions using electron tomography.
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spelling From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glassBiogranbiointerphaseelectron tomographyfocused ion beamosseointegrationscanning transmission electron microscopyThe success of biomaterials for bone regeneration relies on many factors, among which osseointegration plays a key role. Biogran (BG) is a bioactive glass commonly employed as a bone graft in dental procedures. Despite its use in clinical practice, the capability of BG to promote osseointegration has never been resolved at the nanoscale. In this paper, we present the workflow for characterizing the interface between newly formed bone and BG in a preclinical rat model. Areas of bone-BG contact were first identified by backscattered electron imaging in a scanning electron microscope. A focused ion beam in situ lift-out protocol was employed to prepare ultrathin samples for transmission electron microscopy analysis. The bone-BG gradual interface, i.e. the biointerphase, was visualized at the nanoscale with unprecedented resolution thanks to scanning transmission electron microscopy. Finally, we present a method to view the bone-BG interface in three dimensions using electron tomography.Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, CanadaDepartment of Surgery and Integrated Clinic São Paulo State University Araçatuba Dental SchoolCanadian Centre for Electron Microscopy, McMaster University, Hamilton, Ontario, CanadaDepartment of Physics São Paulo State University School of SciencesDepartment of Basic Sciences São Paulo State University Araçatuba Dental SchoolResearch Productivity Scholarship (Process: 309408/2020-2)School of Biomedical Engineering, McMaster University, Hamilton, Ontario, CanadaDepartment of Surgery and Integrated Clinic São Paulo State University Araçatuba Dental SchoolDepartment of Physics São Paulo State University School of SciencesDepartment of Basic Sciences São Paulo State University Araçatuba Dental SchoolUniversidade Estadual Paulista (UNESP)Research Productivity Scholarship (Process: 309408/2020-2)Micheletti, ChiaraGomes-Ferreira, Pedro Henrique Silva [UNESP]Casagrande, TravisLisboa-Filho, Paulo Noronha [UNESP]Okamoto, Roberta [UNESP]Grandfield, Kathryn2022-04-29T08:34:49Z2022-04-29T08:34:49Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20210181http://dx.doi.org/10.1098/rsif.2021.0181Journal of the Royal Society, Interface, v. 18, n. 182, p. 20210181-, 2021.1742-5662http://hdl.handle.net/11449/22961210.1098/rsif.2021.01812-s2.0-85115967055Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Royal Society, Interfaceinfo:eu-repo/semantics/openAccess2022-04-29T08:34:49Zoai:repositorio.unesp.br:11449/229612Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:34:16.257894Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
title From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
spellingShingle From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
Micheletti, Chiara
Biogran
biointerphase
electron tomography
focused ion beam
osseointegration
scanning transmission electron microscopy
title_short From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
title_full From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
title_fullStr From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
title_full_unstemmed From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
title_sort From tissue retrieval to electron tomography: nanoscale characterization of the interface between bone and bioactive glass
author Micheletti, Chiara
author_facet Micheletti, Chiara
Gomes-Ferreira, Pedro Henrique Silva [UNESP]
Casagrande, Travis
Lisboa-Filho, Paulo Noronha [UNESP]
Okamoto, Roberta [UNESP]
Grandfield, Kathryn
author_role author
author2 Gomes-Ferreira, Pedro Henrique Silva [UNESP]
Casagrande, Travis
Lisboa-Filho, Paulo Noronha [UNESP]
Okamoto, Roberta [UNESP]
Grandfield, Kathryn
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Research Productivity Scholarship (Process: 309408/2020-2)
dc.contributor.author.fl_str_mv Micheletti, Chiara
Gomes-Ferreira, Pedro Henrique Silva [UNESP]
Casagrande, Travis
Lisboa-Filho, Paulo Noronha [UNESP]
Okamoto, Roberta [UNESP]
Grandfield, Kathryn
dc.subject.por.fl_str_mv Biogran
biointerphase
electron tomography
focused ion beam
osseointegration
scanning transmission electron microscopy
topic Biogran
biointerphase
electron tomography
focused ion beam
osseointegration
scanning transmission electron microscopy
description The success of biomaterials for bone regeneration relies on many factors, among which osseointegration plays a key role. Biogran (BG) is a bioactive glass commonly employed as a bone graft in dental procedures. Despite its use in clinical practice, the capability of BG to promote osseointegration has never been resolved at the nanoscale. In this paper, we present the workflow for characterizing the interface between newly formed bone and BG in a preclinical rat model. Areas of bone-BG contact were first identified by backscattered electron imaging in a scanning electron microscope. A focused ion beam in situ lift-out protocol was employed to prepare ultrathin samples for transmission electron microscopy analysis. The bone-BG gradual interface, i.e. the biointerphase, was visualized at the nanoscale with unprecedented resolution thanks to scanning transmission electron microscopy. Finally, we present a method to view the bone-BG interface in three dimensions using electron tomography.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-01
2022-04-29T08:34:49Z
2022-04-29T08:34:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1098/rsif.2021.0181
Journal of the Royal Society, Interface, v. 18, n. 182, p. 20210181-, 2021.
1742-5662
http://hdl.handle.net/11449/229612
10.1098/rsif.2021.0181
2-s2.0-85115967055
url http://dx.doi.org/10.1098/rsif.2021.0181
http://hdl.handle.net/11449/229612
identifier_str_mv Journal of the Royal Society, Interface, v. 18, n. 182, p. 20210181-, 2021.
1742-5662
10.1098/rsif.2021.0181
2-s2.0-85115967055
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Royal Society, Interface
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20210181
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128950377906176

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