KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.redalyc.org/articulo.oa?id=33917977012 http://hdl.handle.net/11449/18694 |
Resumo: | The first cytogenetic analysis by conventional Giemsa staining silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, I subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: one coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a pen-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair N(o) 3. Chromosome evolution in Synbranchidae is discussed. |
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Repositório Institucional da UNESP |
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KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELAChromosome EvolutionChromosome Fusion18S DNAFish ChromosomePericentric Inversion5S rDNAThe first cytogenetic analysis by conventional Giemsa staining silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, I subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: one coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a pen-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair N(o) 3. Chromosome evolution in Synbranchidae is discussed.Consejo de Investigacion, Universidad de Oriente, VenezuelaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista UNESP Botucatu, São Paulo, BrazilUNESP Botucatu, Botucatu, SP, BrazilUniv Estadual Campinas, BR-13081970 Campinas, SP, BrazilUniv Estadual Paulista, São Paulo, BrazilUniv São Paulo, BR-05508 São Paulo, BrazilUniv Estadual Paulista UNESP Botucatu, São Paulo, BrazilUNESP Botucatu, Botucatu, SP, BrazilUniv Estadual Paulista, São Paulo, BrazilIntercienciaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Nirchio, Mauro [UNESP]Casagrande Mariguela, Tatiane [UNESP]Alves Ferreira, Irani [UNESP]Foresti, Fausto [UNESP]Oliveira, Claudio [UNESP]2014-05-20T13:52:18Z2014-05-20T13:52:18Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article229-233http://www.redalyc.org/articulo.oa?id=33917977012Interciencia. Caracas: Interciencia, v. 36, n. 3, p. 229-233, 2011.0378-1844http://hdl.handle.net/11449/18694WOS:0002891256000110804793944846367Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInterciencia0.2580,183info:eu-repo/semantics/openAccess2021-10-23T10:49:12Zoai:repositorio.unesp.br:11449/18694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:04:33.134348Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
title |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
spellingShingle |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA Nirchio, Mauro [UNESP] Chromosome Evolution Chromosome Fusion 18S DNA Fish Chromosome Pericentric Inversion 5S rDNA |
title_short |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
title_full |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
title_fullStr |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
title_full_unstemmed |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
title_sort |
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA |
author |
Nirchio, Mauro [UNESP] |
author_facet |
Nirchio, Mauro [UNESP] Casagrande Mariguela, Tatiane [UNESP] Alves Ferreira, Irani [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
author_role |
author |
author2 |
Casagrande Mariguela, Tatiane [UNESP] Alves Ferreira, Irani [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nirchio, Mauro [UNESP] Casagrande Mariguela, Tatiane [UNESP] Alves Ferreira, Irani [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome Evolution Chromosome Fusion 18S DNA Fish Chromosome Pericentric Inversion 5S rDNA |
topic |
Chromosome Evolution Chromosome Fusion 18S DNA Fish Chromosome Pericentric Inversion 5S rDNA |
description |
The first cytogenetic analysis by conventional Giemsa staining silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, I subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: one coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a pen-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair N(o) 3. Chromosome evolution in Synbranchidae is discussed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2014-05-20T13:52:18Z 2014-05-20T13:52:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.redalyc.org/articulo.oa?id=33917977012 Interciencia. Caracas: Interciencia, v. 36, n. 3, p. 229-233, 2011. 0378-1844 http://hdl.handle.net/11449/18694 WOS:000289125600011 0804793944846367 |
url |
http://www.redalyc.org/articulo.oa?id=33917977012 http://hdl.handle.net/11449/18694 |
identifier_str_mv |
Interciencia. Caracas: Interciencia, v. 36, n. 3, p. 229-233, 2011. 0378-1844 WOS:000289125600011 0804793944846367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Interciencia 0.258 0,183 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
229-233 |
dc.publisher.none.fl_str_mv |
Interciencia |
publisher.none.fl_str_mv |
Interciencia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128454794674176 |