A putative role of teneurin-2 and its related proteins in astrocytes
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fnins.2019.00655 http://hdl.handle.net/11449/189369 |
Resumo: | Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS. |
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A putative role of teneurin-2 and its related proteins in astrocytesAdult ratCerebral cortexLatrophilinMechanical brain injuryReactive astrocytesTeneurinTeneurin c-terminal associated peptideTeneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Natural Sciences and Engineering Research Council of CanadaDepartment of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP)Department of Anatomy Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Cell and Systems Biology University of TorontoSchool of Medicine Federal University of Mato Grosso do Sul (UFMS)Department of Anatomy Institute of Biomedical Sciences São Paulo University (USP)Department of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP)Department of Anatomy Institute of Biosciences of Botucatu São Paulo State University (UNESP)CAPES: 001FAPESP: 2008/02771-6FAPESP: 2009/54141-9FAPESP: 2010/52068-0FAPESP: 2012/03067-6FAPESP: 2012/08833-9FAPESP: 2013/26779-4Universidade Estadual Paulista (Unesp)University of TorontoUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade de São Paulo (USP)Tessarin, Gestter W.L. [UNESP]Michalec, Ola M.Torres-Da-Silva, Kelly R. [UNESP]Da Silva, André V. [UNESP]Cruz-Rizzolo, Roelf J. [UNESP]Gonçalves, Alaide [UNESP]Gasparini, Daniele C. [UNESP]Horta-Júnior, José A.C. [UNESP]Ervolino, Edilson [UNESP]Bittencourt, Jackson C.Lovejoy, David A.Casatti, Cláudio A. [UNESP]2019-10-06T16:38:24Z2019-10-06T16:38:24Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fnins.2019.00655Frontiers in Neuroscience, v. 13, n. JUN, 2019.1662-453X1662-4548http://hdl.handle.net/11449/18936910.3389/fnins.2019.006552-s2.0-85068466489440809551734684684874626269318770000-0003-4859-05830000-0001-5650-7343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Neuroscienceinfo:eu-repo/semantics/openAccess2021-12-14T13:51:55Zoai:repositorio.unesp.br:11449/189369Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:48:33.395043Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A putative role of teneurin-2 and its related proteins in astrocytes |
title |
A putative role of teneurin-2 and its related proteins in astrocytes |
spellingShingle |
A putative role of teneurin-2 and its related proteins in astrocytes Tessarin, Gestter W.L. [UNESP] Adult rat Cerebral cortex Latrophilin Mechanical brain injury Reactive astrocytes Teneurin Teneurin c-terminal associated peptide |
title_short |
A putative role of teneurin-2 and its related proteins in astrocytes |
title_full |
A putative role of teneurin-2 and its related proteins in astrocytes |
title_fullStr |
A putative role of teneurin-2 and its related proteins in astrocytes |
title_full_unstemmed |
A putative role of teneurin-2 and its related proteins in astrocytes |
title_sort |
A putative role of teneurin-2 and its related proteins in astrocytes |
author |
Tessarin, Gestter W.L. [UNESP] |
author_facet |
Tessarin, Gestter W.L. [UNESP] Michalec, Ola M. Torres-Da-Silva, Kelly R. [UNESP] Da Silva, André V. [UNESP] Cruz-Rizzolo, Roelf J. [UNESP] Gonçalves, Alaide [UNESP] Gasparini, Daniele C. [UNESP] Horta-Júnior, José A.C. [UNESP] Ervolino, Edilson [UNESP] Bittencourt, Jackson C. Lovejoy, David A. Casatti, Cláudio A. [UNESP] |
author_role |
author |
author2 |
Michalec, Ola M. Torres-Da-Silva, Kelly R. [UNESP] Da Silva, André V. [UNESP] Cruz-Rizzolo, Roelf J. [UNESP] Gonçalves, Alaide [UNESP] Gasparini, Daniele C. [UNESP] Horta-Júnior, José A.C. [UNESP] Ervolino, Edilson [UNESP] Bittencourt, Jackson C. Lovejoy, David A. Casatti, Cláudio A. [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Toronto Universidade Federal de Mato Grosso do Sul (UFMS) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Tessarin, Gestter W.L. [UNESP] Michalec, Ola M. Torres-Da-Silva, Kelly R. [UNESP] Da Silva, André V. [UNESP] Cruz-Rizzolo, Roelf J. [UNESP] Gonçalves, Alaide [UNESP] Gasparini, Daniele C. [UNESP] Horta-Júnior, José A.C. [UNESP] Ervolino, Edilson [UNESP] Bittencourt, Jackson C. Lovejoy, David A. Casatti, Cláudio A. [UNESP] |
dc.subject.por.fl_str_mv |
Adult rat Cerebral cortex Latrophilin Mechanical brain injury Reactive astrocytes Teneurin Teneurin c-terminal associated peptide |
topic |
Adult rat Cerebral cortex Latrophilin Mechanical brain injury Reactive astrocytes Teneurin Teneurin c-terminal associated peptide |
description |
Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:38:24Z 2019-10-06T16:38:24Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fnins.2019.00655 Frontiers in Neuroscience, v. 13, n. JUN, 2019. 1662-453X 1662-4548 http://hdl.handle.net/11449/189369 10.3389/fnins.2019.00655 2-s2.0-85068466489 4408095517346846 8487462626931877 0000-0003-4859-0583 0000-0001-5650-7343 |
url |
http://dx.doi.org/10.3389/fnins.2019.00655 http://hdl.handle.net/11449/189369 |
identifier_str_mv |
Frontiers in Neuroscience, v. 13, n. JUN, 2019. 1662-453X 1662-4548 10.3389/fnins.2019.00655 2-s2.0-85068466489 4408095517346846 8487462626931877 0000-0003-4859-0583 0000-0001-5650-7343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Neuroscience |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128982515712000 |