QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s40858-020-00372-7 http://hdl.handle.net/11449/221484 |
Resumo: | Huanglongbing (HLB) is a severe disease of citrus caused by the bacterium Candidatus Liberibacter. In America, the most common species is Candidatus Liberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization between Citrus sunki Hort. Ex Tan. and Poncirus trifoliata (L.) Raf. cv. Rubidoux. The bacterial concentration was higher in C. sunki than in P. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps of C. sunki and P. trifoliata cv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) between C. sunki and P. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in the C. sunki map and 17 QTL and 40 eQTL in the P. trifoliata map. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. |
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QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarinsCitrandarinsGene expressionHuanglongbingPhenotypic analysisHuanglongbing (HLB) is a severe disease of citrus caused by the bacterium Candidatus Liberibacter. In America, the most common species is Candidatus Liberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization between Citrus sunki Hort. Ex Tan. and Poncirus trifoliata (L.) Raf. cv. Rubidoux. The bacterial concentration was higher in C. sunki than in P. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps of C. sunki and P. trifoliata cv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) between C. sunki and P. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in the C. sunki map and 17 QTL and 40 eQTL in the P. trifoliata map. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection.Centro APTA Citros Sylvio Moreira Instituto Agronômico de CampinasLaboratório de Bioinformática Centro de Ciências Biológicas Universidade Federal de Santa CatarinaUniversidade Estadual de CampinasHill LaboratoriesDepartamento de Genética Instituto de Biociências Universidade do Estado de São PauloCentro de Ciências Agrárias Universidade Federal de São CarlosInstituto Agronômico de CampinasUniversidade Federal de Santa Catarina (UFSC)Universidade Estadual de Campinas (UNICAMP)Hill LaboratoriesUniversidade do Estado de São PauloUniversidade Federal de São Carlos (UFSCar)Soratto, Tatiany Aparecida TeixeiraCurtolo, MaiaraMarengo, SamantaDezotti, Ana LúciaLima, Rômulo Pedro MacêdoGazaffi, RodrigoMachado, Marcos AntonioCristofani-Yaly, Mariângela2022-04-28T19:28:40Z2022-04-28T19:28:40Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article626-645http://dx.doi.org/10.1007/s40858-020-00372-7Tropical Plant Pathology, v. 45, n. 6, p. 626-645, 2020.1983-20521982-5676http://hdl.handle.net/11449/22148410.1007/s40858-020-00372-72-s2.0-85085592450Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Plant Pathologyinfo:eu-repo/semantics/openAccess2022-04-28T19:28:40Zoai:repositorio.unesp.br:11449/221484Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:28:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
title |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
spellingShingle |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins Soratto, Tatiany Aparecida Teixeira Citrandarins Gene expression Huanglongbing Phenotypic analysis |
title_short |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
title_full |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
title_fullStr |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
title_full_unstemmed |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
title_sort |
QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins |
author |
Soratto, Tatiany Aparecida Teixeira |
author_facet |
Soratto, Tatiany Aparecida Teixeira Curtolo, Maiara Marengo, Samanta Dezotti, Ana Lúcia Lima, Rômulo Pedro Macêdo Gazaffi, Rodrigo Machado, Marcos Antonio Cristofani-Yaly, Mariângela |
author_role |
author |
author2 |
Curtolo, Maiara Marengo, Samanta Dezotti, Ana Lúcia Lima, Rômulo Pedro Macêdo Gazaffi, Rodrigo Machado, Marcos Antonio Cristofani-Yaly, Mariângela |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Agronômico de Campinas Universidade Federal de Santa Catarina (UFSC) Universidade Estadual de Campinas (UNICAMP) Hill Laboratories Universidade do Estado de São Paulo Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Soratto, Tatiany Aparecida Teixeira Curtolo, Maiara Marengo, Samanta Dezotti, Ana Lúcia Lima, Rômulo Pedro Macêdo Gazaffi, Rodrigo Machado, Marcos Antonio Cristofani-Yaly, Mariângela |
dc.subject.por.fl_str_mv |
Citrandarins Gene expression Huanglongbing Phenotypic analysis |
topic |
Citrandarins Gene expression Huanglongbing Phenotypic analysis |
description |
Huanglongbing (HLB) is a severe disease of citrus caused by the bacterium Candidatus Liberibacter. In America, the most common species is Candidatus Liberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization between Citrus sunki Hort. Ex Tan. and Poncirus trifoliata (L.) Raf. cv. Rubidoux. The bacterial concentration was higher in C. sunki than in P. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps of C. sunki and P. trifoliata cv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) between C. sunki and P. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in the C. sunki map and 17 QTL and 40 eQTL in the P. trifoliata map. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2022-04-28T19:28:40Z 2022-04-28T19:28:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s40858-020-00372-7 Tropical Plant Pathology, v. 45, n. 6, p. 626-645, 2020. 1983-2052 1982-5676 http://hdl.handle.net/11449/221484 10.1007/s40858-020-00372-7 2-s2.0-85085592450 |
url |
http://dx.doi.org/10.1007/s40858-020-00372-7 http://hdl.handle.net/11449/221484 |
identifier_str_mv |
Tropical Plant Pathology, v. 45, n. 6, p. 626-645, 2020. 1983-2052 1982-5676 10.1007/s40858-020-00372-7 2-s2.0-85085592450 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tropical Plant Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
626-645 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650226116886528 |