Bioprospecção de atividade antioxidante e antimicrobiana da casca de Stryphnodendron adstringens (Mart.) Coville (Leguminosae-Mimosoidae)

• Autor(a) principal: De Souza, Tatiana Maria [UNESP]
• Data de Publicação: 2007
• Outros Autores: Severi, J. A. [UNESP], Silva, V. Y. A. [UNESP], Santos, E. [UNESP], Pietro, R. C. L. R. [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/334; http://hdl.handle.net/11449/70142
• topic: Antimicrobial activity; Antioxidant activity; Stryphnodendron adstringens; Tannins; 1,1 diphenyl 2 picrylhydrazyl; acetone; agar; alcohol; chloroform; drug metabolite; plant extract; Stryphnodendron adstringens extract; tannin; unclassified drug; water; agar diffusion; antibacterial activity; antimicrobial activity; antioxidant activity; bacterial growth; bark; bioautography; Brazil; comparative study; controlled study; drug isolation; drug screening; growth inhibition; legume; measurement; nonhuman; spectrophotometry; thin layer chromatography; traditional medicine

The aim of this work was to evaluate the class of secondary metabolites responsible for the antioxidant and antimicrobial activities of bark extracts of Stryphnodendron adstringens (Mart.) Coville (Leguminosae-Mimosoidae), a plant widely used in folk medicine in Brazil. Extracts of the bark were prepared with 50% ethanol, 70% ethanol, acetone:water (7:3, v/v) and chloroform. Antioxidant activity was prospected by spraying thin-layer chromatographs of the extracts with 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and measuring the DPPH radical scavenging capacity by spectrophotometry. Antibacterial activity was revealed by the agar diffusion method and bioautography. TLC spots assigned to tannins in the polar extracts showed antioxidant activity by DPPH radical scavenging and the chloroform extract showed the least scavenging activity. Antimicrobial activity was indicated by the bacterial growth inhibition haloes around polar extracts and bioautography showed activity in the TLC spots assigned to tannins. It was concluded that polar extracts of the bark of S. adstringens possessed antioxidant and antimicrobial activities which were due to secondary metabolite derived from the tannin class, which are the main constituent of these bark extracts, according to the literature.