Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jat.4384 http://hdl.handle.net/11449/242245 |
Resumo: | 3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 μg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 μg/ml, which encourages further studies aiming to explore its potential as a drug candidate. |
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Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes3-hydroxyphenylcoumarinscomet assaygenotoxic assessmentmicronucleus testsynthetic coumarin3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 μg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 μg/ml, which encourages further studies aiming to explore its potential as a drug candidate.Marilia Medical School FAMEMAUniversidad Estatal AmazonicaDepartment of Organic Chemistry Faculty of Pharmacy University of Santiago de CompostelaCIQUP/Department of Chemistry and Biochemistry Faculty of Sciences University of PortoFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University—UNESPFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University—UNESPFAMEMAUniversidad Estatal AmazonicaUniversity of Santiago de CompostelaUniversity of PortoUniversidade Estadual Paulista (UNESP)Castanha, Ana Paula MartinsAlmeida-Terassi, Laíza MouraGuardado-Yordi, EstelaMatos, Maria JoãoMaistro, Edson Luis [UNESP]2023-03-02T12:25:26Z2023-03-02T12:25:26Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jat.4384Journal of Applied Toxicology.1099-12630260-437Xhttp://hdl.handle.net/11449/24224510.1002/jat.43842-s2.0-85137350042Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Toxicologyinfo:eu-repo/semantics/openAccess2024-08-09T17:40:01Zoai:repositorio.unesp.br:11449/242245Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T17:40:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
title |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
spellingShingle |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes Castanha, Ana Paula Martins 3-hydroxyphenylcoumarins comet assay genotoxic assessment micronucleus test synthetic coumarin |
title_short |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
title_full |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
title_fullStr |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
title_full_unstemmed |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
title_sort |
Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes |
author |
Castanha, Ana Paula Martins |
author_facet |
Castanha, Ana Paula Martins Almeida-Terassi, Laíza Moura Guardado-Yordi, Estela Matos, Maria João Maistro, Edson Luis [UNESP] |
author_role |
author |
author2 |
Almeida-Terassi, Laíza Moura Guardado-Yordi, Estela Matos, Maria João Maistro, Edson Luis [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
FAMEMA Universidad Estatal Amazonica University of Santiago de Compostela University of Porto Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Castanha, Ana Paula Martins Almeida-Terassi, Laíza Moura Guardado-Yordi, Estela Matos, Maria João Maistro, Edson Luis [UNESP] |
dc.subject.por.fl_str_mv |
3-hydroxyphenylcoumarins comet assay genotoxic assessment micronucleus test synthetic coumarin |
topic |
3-hydroxyphenylcoumarins comet assay genotoxic assessment micronucleus test synthetic coumarin |
description |
3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 μg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 μg/ml, which encourages further studies aiming to explore its potential as a drug candidate. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T12:25:26Z 2023-03-02T12:25:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jat.4384 Journal of Applied Toxicology. 1099-1263 0260-437X http://hdl.handle.net/11449/242245 10.1002/jat.4384 2-s2.0-85137350042 |
url |
http://dx.doi.org/10.1002/jat.4384 http://hdl.handle.net/11449/242245 |
identifier_str_mv |
Journal of Applied Toxicology. 1099-1263 0260-437X 10.1002/jat.4384 2-s2.0-85137350042 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128196125655040 |