In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1007/s11356-020-07602-0 http://hdl.handle.net/11449/199989 |
Resumo: | The aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro. |
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In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuaryComet assayCytotoxicityGenotoxicityMicronucleus assaySantos-São Vicente estuaryThe aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Biociências Universidade Federal de São Paulo UNIFESP, Av. Ana Costa, 95, Vila MathiasDepartment of Pathology Botucatu Medical School São Paulo State University UNESPDepartment of Sea Sciences Federal University of São Paulo UNIFESP Campus Baixada SantistaDepartment of Pathology Botucatu Medical School São Paulo State University UNESPCNPq: 001Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Alpire, Maria Esther Suarezde Camargo, Elaine Aparecida [UNESP]Cardoso, Caroline MargonatoSalvadori, Daisy Maria Favero [UNESP]Pereira, Camilo Dias SeabraRibeiro, Daniel Araki2020-12-12T01:54:41Z2020-12-12T01:54:41Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12039-12046http://dx.doi.org/10.1007/s11356-020-07602-0Environmental Science and Pollution Research, v. 27, n. 11, p. 12039-12046, 2020.1614-74990944-1344http://hdl.handle.net/11449/19998910.1007/s11356-020-07602-02-s2.0-85078341286Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2021-10-23T10:18:41Zoai:repositorio.unesp.br:11449/199989Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| title |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| spellingShingle |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary Alpire, Maria Esther Suarez Comet assay Cytotoxicity Genotoxicity Micronucleus assay Santos-São Vicente estuary |
| title_short |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| title_full |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| title_fullStr |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| title_full_unstemmed |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| title_sort |
In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary |
| author |
Alpire, Maria Esther Suarez |
| author_facet |
Alpire, Maria Esther Suarez de Camargo, Elaine Aparecida [UNESP] Cardoso, Caroline Margonato Salvadori, Daisy Maria Favero [UNESP] Pereira, Camilo Dias Seabra Ribeiro, Daniel Araki |
| author_role |
author |
| author2 |
de Camargo, Elaine Aparecida [UNESP] Cardoso, Caroline Margonato Salvadori, Daisy Maria Favero [UNESP] Pereira, Camilo Dias Seabra Ribeiro, Daniel Araki |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
| dc.contributor.author.fl_str_mv |
Alpire, Maria Esther Suarez de Camargo, Elaine Aparecida [UNESP] Cardoso, Caroline Margonato Salvadori, Daisy Maria Favero [UNESP] Pereira, Camilo Dias Seabra Ribeiro, Daniel Araki |
| dc.subject.por.fl_str_mv |
Comet assay Cytotoxicity Genotoxicity Micronucleus assay Santos-São Vicente estuary |
| topic |
Comet assay Cytotoxicity Genotoxicity Micronucleus assay Santos-São Vicente estuary |
| description |
The aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-12T01:54:41Z 2020-12-12T01:54:41Z 2020-04-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-020-07602-0 Environmental Science and Pollution Research, v. 27, n. 11, p. 12039-12046, 2020. 1614-7499 0944-1344 http://hdl.handle.net/11449/199989 10.1007/s11356-020-07602-0 2-s2.0-85078341286 |
| url |
http://dx.doi.org/10.1007/s11356-020-07602-0 http://hdl.handle.net/11449/199989 |
| identifier_str_mv |
Environmental Science and Pollution Research, v. 27, n. 11, p. 12039-12046, 2020. 1614-7499 0944-1344 10.1007/s11356-020-07602-0 2-s2.0-85078341286 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
12039-12046 |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
|
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1803649457186668544 |