Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Detalhes bibliográficos
Autor(a) principal: Freitas, Telma Luisa de [UNESP]
Data de Publicação: 2004
Outros Autores: Monti, Rubens [UNESP], Contiero, Jonas [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822004000200015
http://hdl.handle.net/11449/67995
Resumo: GCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition.
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spelling Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industryβ-cyclodextrinBacillus alkalophilic CGIICGTaseEnzyme activityStarch hydrolysisManihot esculentaGCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition.Departamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SPInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SPDepartamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SPInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SPUniversidade Estadual Paulista (Unesp)Freitas, Telma Luisa de [UNESP]Monti, Rubens [UNESP]Contiero, Jonas [UNESP]2014-05-27T11:21:12Z2014-05-27T11:21:12Z2004-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article255-260application/pdfhttp://dx.doi.org/10.1590/S1517-83822004000200015Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004.1517-8382http://hdl.handle.net/11449/6799510.1590/S1517-83822004000200015S1517-83822004000200015WOS:0002348026000152-s2.0-199443632252-s2.0-19944363225.pdf9859154979447005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2023-12-22T06:24:06Zoai:repositorio.unesp.br:11449/67995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-22T06:24:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
spellingShingle Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
Freitas, Telma Luisa de [UNESP]
β-cyclodextrin
Bacillus alkalophilic CGII
CGTase
Enzyme activity
Starch hydrolysis
Manihot esculenta
title_short Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_full Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_fullStr Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_full_unstemmed Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_sort Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
author Freitas, Telma Luisa de [UNESP]
author_facet Freitas, Telma Luisa de [UNESP]
Monti, Rubens [UNESP]
Contiero, Jonas [UNESP]
author_role author
author2 Monti, Rubens [UNESP]
Contiero, Jonas [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Freitas, Telma Luisa de [UNESP]
Monti, Rubens [UNESP]
Contiero, Jonas [UNESP]
dc.subject.por.fl_str_mv β-cyclodextrin
Bacillus alkalophilic CGII
CGTase
Enzyme activity
Starch hydrolysis
Manihot esculenta
topic β-cyclodextrin
Bacillus alkalophilic CGII
CGTase
Enzyme activity
Starch hydrolysis
Manihot esculenta
description GCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition.
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
2014-05-27T11:21:12Z
2014-05-27T11:21:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822004000200015
Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004.
1517-8382
http://hdl.handle.net/11449/67995
10.1590/S1517-83822004000200015
S1517-83822004000200015
WOS:000234802600015
2-s2.0-19944363225
2-s2.0-19944363225.pdf
9859154979447005
url http://dx.doi.org/10.1590/S1517-83822004000200015
http://hdl.handle.net/11449/67995
identifier_str_mv Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004.
1517-8382
10.1590/S1517-83822004000200015
S1517-83822004000200015
WOS:000234802600015
2-s2.0-19944363225
2-s2.0-19944363225.pdf
9859154979447005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 255-260
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965371371880448

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