Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822004000200015 http://hdl.handle.net/11449/67995 |
Resumo: | GCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition. |
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Repositório Institucional da UNESP |
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spelling |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industryβ-cyclodextrinBacillus alkalophilic CGIICGTaseEnzyme activityStarch hydrolysisManihot esculentaGCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition.Departamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SPInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SPDepartamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SPDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SPInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SPUniversidade Estadual Paulista (Unesp)Freitas, Telma Luisa de [UNESP]Monti, Rubens [UNESP]Contiero, Jonas [UNESP]2014-05-27T11:21:12Z2014-05-27T11:21:12Z2004-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article255-260application/pdfhttp://dx.doi.org/10.1590/S1517-83822004000200015Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004.1517-8382http://hdl.handle.net/11449/6799510.1590/S1517-83822004000200015S1517-83822004000200015WOS:0002348026000152-s2.0-199443632252-s2.0-19944363225.pdf9859154979447005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-06-21T12:47:11Zoai:repositorio.unesp.br:11449/67995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:02.294581Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
spellingShingle |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry Freitas, Telma Luisa de [UNESP] β-cyclodextrin Bacillus alkalophilic CGII CGTase Enzyme activity Starch hydrolysis Manihot esculenta |
title_short |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_full |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_fullStr |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_full_unstemmed |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_sort |
Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
author |
Freitas, Telma Luisa de [UNESP] |
author_facet |
Freitas, Telma Luisa de [UNESP] Monti, Rubens [UNESP] Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Monti, Rubens [UNESP] Contiero, Jonas [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Freitas, Telma Luisa de [UNESP] Monti, Rubens [UNESP] Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
β-cyclodextrin Bacillus alkalophilic CGII CGTase Enzyme activity Starch hydrolysis Manihot esculenta |
topic |
β-cyclodextrin Bacillus alkalophilic CGII CGTase Enzyme activity Starch hydrolysis Manihot esculenta |
description |
GCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-12-01 2014-05-27T11:21:12Z 2014-05-27T11:21:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822004000200015 Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004. 1517-8382 http://hdl.handle.net/11449/67995 10.1590/S1517-83822004000200015 S1517-83822004000200015 WOS:000234802600015 2-s2.0-19944363225 2-s2.0-19944363225.pdf 9859154979447005 |
url |
http://dx.doi.org/10.1590/S1517-83822004000200015 http://hdl.handle.net/11449/67995 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004. 1517-8382 10.1590/S1517-83822004000200015 S1517-83822004000200015 WOS:000234802600015 2-s2.0-19944363225 2-s2.0-19944363225.pdf 9859154979447005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
255-260 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129275705950208 |