Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Detalhes bibliográficos
Autor(a) principal: Freitas,Telma Luisa de
Data de Publicação: 2004
Outros Autores: Monti,Rubens, Contiero,Jonas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015
Resumo: GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.
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spelling Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industryBacillus alkalophilic CGIICGTasebeta-cyclodextrinstarch hydrolysisenzyme activityGCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.Sociedade Brasileira de Microbiologia2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015Brazilian Journal of Microbiology v.35 n.3 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000200015info:eu-repo/semantics/openAccessFreitas,Telma Luisa deMonti,RubensContiero,Jonaseng2005-11-25T00:00:00Zoai:scielo:S1517-83822004000200015Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-11-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
spellingShingle Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
Freitas,Telma Luisa de
Bacillus alkalophilic CGII
CGTase
beta-cyclodextrin
starch hydrolysis
enzyme activity
title_short Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_full Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_fullStr Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_full_unstemmed Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
title_sort Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
author Freitas,Telma Luisa de
author_facet Freitas,Telma Luisa de
Monti,Rubens
Contiero,Jonas
author_role author
author2 Monti,Rubens
Contiero,Jonas
author2_role author
author
dc.contributor.author.fl_str_mv Freitas,Telma Luisa de
Monti,Rubens
Contiero,Jonas
dc.subject.por.fl_str_mv Bacillus alkalophilic CGII
CGTase
beta-cyclodextrin
starch hydrolysis
enzyme activity
topic Bacillus alkalophilic CGII
CGTase
beta-cyclodextrin
starch hydrolysis
enzyme activity
description GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822004000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.35 n.3 2004
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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