CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.18066/revistaunivap.v25i48.2198 http://hdl.handle.net/11449/197662 |
Resumo: | Natural products such as glycolic extracts of plants are important for the clinical application in health care, like mouthwashes, toothpastes and intracanal irrigation. It is therefore necessary to perform cytotoxicity studies of these glycolic extracts. Objectives: This study aimed to assess the cytotoxic activity of Cynara scolymus (artichoke), Myracrodruom urundeuva Allem. (aroeira-do-sertao) and Camellia sinensis (L.) Kuntze (green tea) glycolic extracts in mouse macrophages (RAW 264.7) by the MTT metabolic activity test. Design: Macrophages were distributed into 96-well plates and exposed to 11 serial dilutions of each extract (200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL 6.25 mg/mL, 3.13 mg/mL, 1.56 mg/mL, 0.78 mg/mL, 0.39 mg/mL and 0.20 mg/mL), with n=8 for each dilution. After 5 min and 24 h of contact, cell viability was assessed using the MTT assay. Results: Given these results, using 5 min of exposure time to the extracts, in ascending order of cell viability reduction follows green tea, with increased cell viability, pepper tree and artichoke. In the MTT assay of the extracts using exposure time of 24 h, it was observed that artichoke extract showed the greatest toxicity, followed by green tea and pepper tree extracts. Statistical analysis was performed by ANOVA and Tukey's test, with a significance level of 5%. Conclusions: Among the extracts, green tea with 12.5mg/mL had interesting results for 5 min and 24 h, since this was the highest concentration and it wasn't cytotoxic to the macrophages, and artichoke was the most cytotoxic to the macrophages in both times (5 min and 24 h). |
id |
UNSP_7d57f4e3f5f4336de774ed3ef89602cd |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/197662 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTSGreen teaartichokepepper treecytotoxic activitymacrophagesNatural products such as glycolic extracts of plants are important for the clinical application in health care, like mouthwashes, toothpastes and intracanal irrigation. It is therefore necessary to perform cytotoxicity studies of these glycolic extracts. Objectives: This study aimed to assess the cytotoxic activity of Cynara scolymus (artichoke), Myracrodruom urundeuva Allem. (aroeira-do-sertao) and Camellia sinensis (L.) Kuntze (green tea) glycolic extracts in mouse macrophages (RAW 264.7) by the MTT metabolic activity test. Design: Macrophages were distributed into 96-well plates and exposed to 11 serial dilutions of each extract (200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL 6.25 mg/mL, 3.13 mg/mL, 1.56 mg/mL, 0.78 mg/mL, 0.39 mg/mL and 0.20 mg/mL), with n=8 for each dilution. After 5 min and 24 h of contact, cell viability was assessed using the MTT assay. Results: Given these results, using 5 min of exposure time to the extracts, in ascending order of cell viability reduction follows green tea, with increased cell viability, pepper tree and artichoke. In the MTT assay of the extracts using exposure time of 24 h, it was observed that artichoke extract showed the greatest toxicity, followed by green tea and pepper tree extracts. Statistical analysis was performed by ANOVA and Tukey's test, with a significance level of 5%. Conclusions: Among the extracts, green tea with 12.5mg/mL had interesting results for 5 min and 24 h, since this was the highest concentration and it wasn't cytotoxic to the macrophages, and artichoke was the most cytotoxic to the macrophages in both times (5 min and 24 h).Univ Anhanguera Sao Paulo, Sao Paulo, BrazilCtr Univ Brascubas, Mogi Das Cruzes, SP, BrazilUniv Estadual Paulista, Sao Paulo, BrazilUniv Estadual Paulista, Posgrad Biopatol Bucal, Sao Paulo, BrazilUniv Estadual Paulista, Sao Paulo, BrazilUniv Estadual Paulista, Posgrad Biopatol Bucal, Sao Paulo, BrazilUniv Vale Paraiba-univapUniv Anhanguera Sao PauloCtr Univ BrascubasUniversidade Estadual Paulista (Unesp)Higa, Karen CristianeJorjao, Adeline LacerdaOliveira, Felipe EduardoOliveira, Jonatas Rafael de [UNESP]Back Brito, Graziella Nuernberg [UNESP]Cardoso Jorge, Antonio Olavo [UNESP]Oliveira, Luciane Dias de [UNESP]2020-12-11T07:55:55Z2020-12-11T07:55:55Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article76-90http://dx.doi.org/10.18066/revistaunivap.v25i48.2198Revista Univap. Sao Jose Dos Campos: Univ Vale Paraiba-univap, v. 25, n. 48, p. 76-90, 2019.1517-3275http://hdl.handle.net/11449/19766210.18066/revistaunivap.v25i48.2198WOS:000511319900007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Univapinfo:eu-repo/semantics/openAccess2021-10-23T10:18:34Zoai:repositorio.unesp.br:11449/197662Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
title |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
spellingShingle |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS Higa, Karen Cristiane Green tea artichoke pepper tree cytotoxic activity macrophages |
title_short |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
title_full |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
title_fullStr |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
title_full_unstemmed |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
title_sort |
CYTOTOXICITY OF Cynara scolymus (ARTICHOKE), Myracrodruom urundeuva (AROEIRA-DO-SERTAO) AND Camellia sinensis (GREEN TEA) GLYCOLIC EXTRACTS |
author |
Higa, Karen Cristiane |
author_facet |
Higa, Karen Cristiane Jorjao, Adeline Lacerda Oliveira, Felipe Eduardo Oliveira, Jonatas Rafael de [UNESP] Back Brito, Graziella Nuernberg [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Oliveira, Luciane Dias de [UNESP] |
author_role |
author |
author2 |
Jorjao, Adeline Lacerda Oliveira, Felipe Eduardo Oliveira, Jonatas Rafael de [UNESP] Back Brito, Graziella Nuernberg [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Oliveira, Luciane Dias de [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Anhanguera Sao Paulo Ctr Univ Brascubas Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Higa, Karen Cristiane Jorjao, Adeline Lacerda Oliveira, Felipe Eduardo Oliveira, Jonatas Rafael de [UNESP] Back Brito, Graziella Nuernberg [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Oliveira, Luciane Dias de [UNESP] |
dc.subject.por.fl_str_mv |
Green tea artichoke pepper tree cytotoxic activity macrophages |
topic |
Green tea artichoke pepper tree cytotoxic activity macrophages |
description |
Natural products such as glycolic extracts of plants are important for the clinical application in health care, like mouthwashes, toothpastes and intracanal irrigation. It is therefore necessary to perform cytotoxicity studies of these glycolic extracts. Objectives: This study aimed to assess the cytotoxic activity of Cynara scolymus (artichoke), Myracrodruom urundeuva Allem. (aroeira-do-sertao) and Camellia sinensis (L.) Kuntze (green tea) glycolic extracts in mouse macrophages (RAW 264.7) by the MTT metabolic activity test. Design: Macrophages were distributed into 96-well plates and exposed to 11 serial dilutions of each extract (200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12.5 mg/mL 6.25 mg/mL, 3.13 mg/mL, 1.56 mg/mL, 0.78 mg/mL, 0.39 mg/mL and 0.20 mg/mL), with n=8 for each dilution. After 5 min and 24 h of contact, cell viability was assessed using the MTT assay. Results: Given these results, using 5 min of exposure time to the extracts, in ascending order of cell viability reduction follows green tea, with increased cell viability, pepper tree and artichoke. In the MTT assay of the extracts using exposure time of 24 h, it was observed that artichoke extract showed the greatest toxicity, followed by green tea and pepper tree extracts. Statistical analysis was performed by ANOVA and Tukey's test, with a significance level of 5%. Conclusions: Among the extracts, green tea with 12.5mg/mL had interesting results for 5 min and 24 h, since this was the highest concentration and it wasn't cytotoxic to the macrophages, and artichoke was the most cytotoxic to the macrophages in both times (5 min and 24 h). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-11T07:55:55Z 2020-12-11T07:55:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.18066/revistaunivap.v25i48.2198 Revista Univap. Sao Jose Dos Campos: Univ Vale Paraiba-univap, v. 25, n. 48, p. 76-90, 2019. 1517-3275 http://hdl.handle.net/11449/197662 10.18066/revistaunivap.v25i48.2198 WOS:000511319900007 |
url |
http://dx.doi.org/10.18066/revistaunivap.v25i48.2198 http://hdl.handle.net/11449/197662 |
identifier_str_mv |
Revista Univap. Sao Jose Dos Campos: Univ Vale Paraiba-univap, v. 25, n. 48, p. 76-90, 2019. 1517-3275 10.18066/revistaunivap.v25i48.2198 WOS:000511319900007 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Univap |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
76-90 |
dc.publisher.none.fl_str_mv |
Univ Vale Paraiba-univap |
publisher.none.fl_str_mv |
Univ Vale Paraiba-univap |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965370579156992 |