Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.indcrop.2021.114043 http://hdl.handle.net/11449/222420 |
Resumo: | Agaves have been used for centuries as a feedstock in dryland areas for fibers, food, and beverages, and have enormous potential for biofuel production. Brazil is the world's largest producer of Agave fiber (sisal). However, since the development of synthetic fibers, the national investment in Agave research has decreased drastically, leading to the cessation of the country's breeding programs. What is left of the Brazilian elite cultivars were planted at a germplasm bank in the middle of the semiarid. Surprisingly, after 7 years of abandonment, the plants were still healthy and did not show any clear signs of stress. Here, we aimed to investigate how these plants managed to cope with this environment and the molecular basis of their biomass traits. We assembled the transcriptomic atlas of Agave sisalana, Agave fourcroydes, and Agave hybrid 11648 ((A. amaniensis x A. angustifolia) x A. amaniensis). We observed that the cultivars activated a highly overlapping set of stress-response genes, which were the most expressed transcripts. Also, raffinose was detected at high concentrations, possibly acting as an osmolyte, though differences at its biosynthesis have been found depending on cultivar. Finally, we observed differences in recalcitrance that could be attributed to lignin composition and its biosynthetic pathway. Our data contribute new insights that can help molecular breeders to correspond to emerging expectations for Agave as biorenewables feedstocks for dryland areas. |
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Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivarsAbiotic stressBiomassGenetic resourcesRNA-SeqSisalAgaves have been used for centuries as a feedstock in dryland areas for fibers, food, and beverages, and have enormous potential for biofuel production. Brazil is the world's largest producer of Agave fiber (sisal). However, since the development of synthetic fibers, the national investment in Agave research has decreased drastically, leading to the cessation of the country's breeding programs. What is left of the Brazilian elite cultivars were planted at a germplasm bank in the middle of the semiarid. Surprisingly, after 7 years of abandonment, the plants were still healthy and did not show any clear signs of stress. Here, we aimed to investigate how these plants managed to cope with this environment and the molecular basis of their biomass traits. We assembled the transcriptomic atlas of Agave sisalana, Agave fourcroydes, and Agave hybrid 11648 ((A. amaniensis x A. angustifolia) x A. amaniensis). We observed that the cultivars activated a highly overlapping set of stress-response genes, which were the most expressed transcripts. Also, raffinose was detected at high concentrations, possibly acting as an osmolyte, though differences at its biosynthesis have been found depending on cultivar. Finally, we observed differences in recalcitrance that could be attributed to lignin composition and its biosynthetic pathway. Our data contribute new insights that can help molecular breeders to correspond to emerging expectations for Agave as biorenewables feedstocks for dryland areas.Laboratório de Genômica e BioEnergia Departamento de Genética Evolução Microbiologia e Imunologia UNICAMPDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas UNESPDepartamento de Botânica Instituto de Biociências USPLaboratório de Anatomia Vegetal Departamento de Biologia Vegetal UNICAMPEmpresa Brasileira de Pesquisa Agropecuária - Algodão EMBRAPAHigh-Throughput Sequencing Facility School of Medicine UNCCentro para Computação em Engenharia e Ciências UNICAMPDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas UNESPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)UNCRaya, Fabio TrigoMarone, Marina PupkeCarvalho, Lucas MiguelRabelo, Sarita Candida [UNESP]de Paula, Maiki SoaresCampanari, Maria Fernanda ZaneliFreschi, LucianoMayer, Juliana Lischka SampaioSilva, Odilon Reny Ribeiro FerreiraMieczkowski, PiotrCarazzolle, Marcelo FalsarellaPereira, Gonçalo Amarante Guimarães2022-04-28T19:44:38Z2022-04-28T19:44:38Z2021-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.indcrop.2021.114043Industrial Crops and Products, v. 172.0926-6690http://hdl.handle.net/11449/22242010.1016/j.indcrop.2021.1140432-s2.0-85114908052Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndustrial Crops and Productsinfo:eu-repo/semantics/openAccess2022-04-28T19:44:38Zoai:repositorio.unesp.br:11449/222420Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:44:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
title |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
spellingShingle |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars Raya, Fabio Trigo Abiotic stress Biomass Genetic resources RNA-Seq Sisal |
title_short |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
title_full |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
title_fullStr |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
title_full_unstemmed |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
title_sort |
Extreme physiology: Biomass and transcriptional profiling of three abandoned Agave cultivars |
author |
Raya, Fabio Trigo |
author_facet |
Raya, Fabio Trigo Marone, Marina Pupke Carvalho, Lucas Miguel Rabelo, Sarita Candida [UNESP] de Paula, Maiki Soares Campanari, Maria Fernanda Zaneli Freschi, Luciano Mayer, Juliana Lischka Sampaio Silva, Odilon Reny Ribeiro Ferreira Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Pereira, Gonçalo Amarante Guimarães |
author_role |
author |
author2 |
Marone, Marina Pupke Carvalho, Lucas Miguel Rabelo, Sarita Candida [UNESP] de Paula, Maiki Soares Campanari, Maria Fernanda Zaneli Freschi, Luciano Mayer, Juliana Lischka Sampaio Silva, Odilon Reny Ribeiro Ferreira Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Pereira, Gonçalo Amarante Guimarães |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) UNC |
dc.contributor.author.fl_str_mv |
Raya, Fabio Trigo Marone, Marina Pupke Carvalho, Lucas Miguel Rabelo, Sarita Candida [UNESP] de Paula, Maiki Soares Campanari, Maria Fernanda Zaneli Freschi, Luciano Mayer, Juliana Lischka Sampaio Silva, Odilon Reny Ribeiro Ferreira Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Pereira, Gonçalo Amarante Guimarães |
dc.subject.por.fl_str_mv |
Abiotic stress Biomass Genetic resources RNA-Seq Sisal |
topic |
Abiotic stress Biomass Genetic resources RNA-Seq Sisal |
description |
Agaves have been used for centuries as a feedstock in dryland areas for fibers, food, and beverages, and have enormous potential for biofuel production. Brazil is the world's largest producer of Agave fiber (sisal). However, since the development of synthetic fibers, the national investment in Agave research has decreased drastically, leading to the cessation of the country's breeding programs. What is left of the Brazilian elite cultivars were planted at a germplasm bank in the middle of the semiarid. Surprisingly, after 7 years of abandonment, the plants were still healthy and did not show any clear signs of stress. Here, we aimed to investigate how these plants managed to cope with this environment and the molecular basis of their biomass traits. We assembled the transcriptomic atlas of Agave sisalana, Agave fourcroydes, and Agave hybrid 11648 ((A. amaniensis x A. angustifolia) x A. amaniensis). We observed that the cultivars activated a highly overlapping set of stress-response genes, which were the most expressed transcripts. Also, raffinose was detected at high concentrations, possibly acting as an osmolyte, though differences at its biosynthesis have been found depending on cultivar. Finally, we observed differences in recalcitrance that could be attributed to lignin composition and its biosynthetic pathway. Our data contribute new insights that can help molecular breeders to correspond to emerging expectations for Agave as biorenewables feedstocks for dryland areas. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-15 2022-04-28T19:44:38Z 2022-04-28T19:44:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.indcrop.2021.114043 Industrial Crops and Products, v. 172. 0926-6690 http://hdl.handle.net/11449/222420 10.1016/j.indcrop.2021.114043 2-s2.0-85114908052 |
url |
http://dx.doi.org/10.1016/j.indcrop.2021.114043 http://hdl.handle.net/11449/222420 |
identifier_str_mv |
Industrial Crops and Products, v. 172. 0926-6690 10.1016/j.indcrop.2021.114043 2-s2.0-85114908052 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Industrial Crops and Products |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964857050595328 |