Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.indcrop.2022.114972 http://hdl.handle.net/11449/239988 |
Resumo: | Sugarcane bagasse (SB) was subjected to enzymatic hydrolysis using an enzyme cocktail immobilized on magnetic graphene oxide particles (GO-MNP). The thermal, storage, pH, and operational stabilities of the immobilized enzymes, including exoglucanase, endoglucanase, β-glucosidase, xylanase, and β-xylosidase, were evaluated. The half-life of the biocatalyst (GO-MNP-Enz) was longer than that of the free enzymes at temperatures above 45 °C, with the exception of endoglucanase. SB was subjected to pretreatments generating sulfite-NaOH pretreated SB (SSB) and chlorite pretreated SB (CSB). The enzymatic hydrolysis of SSB and CSB was evaluated using free enzymes and GO-MNP-Enz. The cellulose and xylan conversion of SSB using free enzymes was higher than using GO-MNP-Enz; however, a similar result was obtained after 72 h. This did not occur in the hydrolysis of CSB, in which at the end of 72 h, conversion using GO-MNP-Enz did not reach the levels obtained with free enzymes. GO-MNP-Enz was successfully reused in several SSB hydrolysis cycles, maintaining an efficiency of approximately 80% and presenting the highest turnover frequency when compared with previous results reported in literature. Finally, these results show that the immobilization of cellulases and xylanases improves their operational stability and the obtained GO-MNP-Enz can be used for various SB hydrolysis cycles. |
id |
UNSP_7d940f888b58d5f49716016feda32130 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/239988 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasseBiocatalyst reuseEnzymatic hydrolysisEnzyme immobilizationMagnetic graphene oxideSecond-generation ethanolSugarcane bagasseSugarcane bagasse (SB) was subjected to enzymatic hydrolysis using an enzyme cocktail immobilized on magnetic graphene oxide particles (GO-MNP). The thermal, storage, pH, and operational stabilities of the immobilized enzymes, including exoglucanase, endoglucanase, β-glucosidase, xylanase, and β-xylosidase, were evaluated. The half-life of the biocatalyst (GO-MNP-Enz) was longer than that of the free enzymes at temperatures above 45 °C, with the exception of endoglucanase. SB was subjected to pretreatments generating sulfite-NaOH pretreated SB (SSB) and chlorite pretreated SB (CSB). The enzymatic hydrolysis of SSB and CSB was evaluated using free enzymes and GO-MNP-Enz. The cellulose and xylan conversion of SSB using free enzymes was higher than using GO-MNP-Enz; however, a similar result was obtained after 72 h. This did not occur in the hydrolysis of CSB, in which at the end of 72 h, conversion using GO-MNP-Enz did not reach the levels obtained with free enzymes. GO-MNP-Enz was successfully reused in several SSB hydrolysis cycles, maintaining an efficiency of approximately 80% and presenting the highest turnover frequency when compared with previous results reported in literature. Finally, these results show that the immobilization of cellulases and xylanases improves their operational stability and the obtained GO-MNP-Enz can be used for various SB hydrolysis cycles.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Agencia Estatal de InvestigaciónSão Paulo State University (UNESP) School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and Biotechnology, SPUniversitat Politècnica de València (UPV) Institute of Chemical Technology (ITQ)National University of Singapore Center for Advanced 2D MaterialsFederal University of Acre Laboratory of Nanobiotechnology, ACFederal University of Rio Grande do Norte Department of Theoretical and Experimental Physics, RNSão Paulo State University (UNESP) School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and Biotechnology, SPFAPESP: 2018/06241–3Agencia Estatal de Investigación: PGC2018-097277-B-100Universidade Estadual Paulista (UNESP)Institute of Chemical Technology (ITQ)Center for Advanced 2D MaterialsLaboratory of NanobiotechnologyFederal University of Rio Grande do NortePaz-Cedeno, Fernando Roberto [UNESP]Carceller, Jose MiguelIborra, SaraDonato, Ricardo KeitelRodriguez, Anselmo Fortunato RuizMorales, Marco AntonioSolorzano-Chavez, Eddyn Gabriel [UNESP]Roldán, Ismael Ulises Miranda [UNESP]Paula, Ariela Veloso de [UNESP]Masarin, Fernando [UNESP]2023-03-01T19:56:36Z2023-03-01T19:56:36Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.indcrop.2022.114972Industrial Crops and Products, v. 183.0926-6690http://hdl.handle.net/11449/23998810.1016/j.indcrop.2022.1149722-s2.0-85129386666Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndustrial Crops and Productsinfo:eu-repo/semantics/openAccess2023-03-01T19:56:37Zoai:repositorio.unesp.br:11449/239988Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T19:56:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
title |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
spellingShingle |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse Paz-Cedeno, Fernando Roberto [UNESP] Biocatalyst reuse Enzymatic hydrolysis Enzyme immobilization Magnetic graphene oxide Second-generation ethanol Sugarcane bagasse |
title_short |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
title_full |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
title_fullStr |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
title_full_unstemmed |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
title_sort |
Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: Assessment of hydrolysis of pretreated sugarcane bagasse |
author |
Paz-Cedeno, Fernando Roberto [UNESP] |
author_facet |
Paz-Cedeno, Fernando Roberto [UNESP] Carceller, Jose Miguel Iborra, Sara Donato, Ricardo Keitel Rodriguez, Anselmo Fortunato Ruiz Morales, Marco Antonio Solorzano-Chavez, Eddyn Gabriel [UNESP] Roldán, Ismael Ulises Miranda [UNESP] Paula, Ariela Veloso de [UNESP] Masarin, Fernando [UNESP] |
author_role |
author |
author2 |
Carceller, Jose Miguel Iborra, Sara Donato, Ricardo Keitel Rodriguez, Anselmo Fortunato Ruiz Morales, Marco Antonio Solorzano-Chavez, Eddyn Gabriel [UNESP] Roldán, Ismael Ulises Miranda [UNESP] Paula, Ariela Veloso de [UNESP] Masarin, Fernando [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Institute of Chemical Technology (ITQ) Center for Advanced 2D Materials Laboratory of Nanobiotechnology Federal University of Rio Grande do Norte |
dc.contributor.author.fl_str_mv |
Paz-Cedeno, Fernando Roberto [UNESP] Carceller, Jose Miguel Iborra, Sara Donato, Ricardo Keitel Rodriguez, Anselmo Fortunato Ruiz Morales, Marco Antonio Solorzano-Chavez, Eddyn Gabriel [UNESP] Roldán, Ismael Ulises Miranda [UNESP] Paula, Ariela Veloso de [UNESP] Masarin, Fernando [UNESP] |
dc.subject.por.fl_str_mv |
Biocatalyst reuse Enzymatic hydrolysis Enzyme immobilization Magnetic graphene oxide Second-generation ethanol Sugarcane bagasse |
topic |
Biocatalyst reuse Enzymatic hydrolysis Enzyme immobilization Magnetic graphene oxide Second-generation ethanol Sugarcane bagasse |
description |
Sugarcane bagasse (SB) was subjected to enzymatic hydrolysis using an enzyme cocktail immobilized on magnetic graphene oxide particles (GO-MNP). The thermal, storage, pH, and operational stabilities of the immobilized enzymes, including exoglucanase, endoglucanase, β-glucosidase, xylanase, and β-xylosidase, were evaluated. The half-life of the biocatalyst (GO-MNP-Enz) was longer than that of the free enzymes at temperatures above 45 °C, with the exception of endoglucanase. SB was subjected to pretreatments generating sulfite-NaOH pretreated SB (SSB) and chlorite pretreated SB (CSB). The enzymatic hydrolysis of SSB and CSB was evaluated using free enzymes and GO-MNP-Enz. The cellulose and xylan conversion of SSB using free enzymes was higher than using GO-MNP-Enz; however, a similar result was obtained after 72 h. This did not occur in the hydrolysis of CSB, in which at the end of 72 h, conversion using GO-MNP-Enz did not reach the levels obtained with free enzymes. GO-MNP-Enz was successfully reused in several SSB hydrolysis cycles, maintaining an efficiency of approximately 80% and presenting the highest turnover frequency when compared with previous results reported in literature. Finally, these results show that the immobilization of cellulases and xylanases improves their operational stability and the obtained GO-MNP-Enz can be used for various SB hydrolysis cycles. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-01T19:56:36Z 2023-03-01T19:56:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.indcrop.2022.114972 Industrial Crops and Products, v. 183. 0926-6690 http://hdl.handle.net/11449/239988 10.1016/j.indcrop.2022.114972 2-s2.0-85129386666 |
url |
http://dx.doi.org/10.1016/j.indcrop.2022.114972 http://hdl.handle.net/11449/239988 |
identifier_str_mv |
Industrial Crops and Products, v. 183. 0926-6690 10.1016/j.indcrop.2022.114972 2-s2.0-85129386666 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Industrial Crops and Products |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789883223244800 |