Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation

Detalhes bibliográficos
Autor(a) principal: Mendes, L. S. [UNESP]
Data de Publicação: 2018
Outros Autores: Saska, S. [UNESP], Coelho, F. [UNESP], Capote, T S De O [UNESP], Scarel-Caminaga, R. M. [UNESP], Marchetto, R. [UNESP], Carrodeguas, R. G., Gaspar, A. M.M. [UNESP], Rodríguez, M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1088/1748-605X/aa9085
http://hdl.handle.net/11449/170722
Resumo: Calcium phosphate cement has been widely investigated as a bone graft substitute due to its excellent self-setting ability, biocompatibility, osteoconductivity and moldability. In addition, mesoporous materials have been studied as potential materials for application in medical devices due to their large surface area, which is capable of loading numerous biological molecules, besides being bioactive. In this study, bone β-TCP-MCPM-based injectable cement with mesoporous silica particles was synthesized and characterized in terms of its mechanical properties, microstructure, porosity, injectability, in vitro bioactivity and degradability; together with toxicity effects in CHO-K1 cell culture. The results showed that the β-TCP-MCPM cement is bioactive after soaking in simulated body fluid solution, and mesoporous silica particles provided better physicochemical properties compared with silica-free cement. Toxicity assays showed low CHO-K1 cell viability after treatment with more concentrated extracts (200 mg ml-1). However, this behavior did not compromise the reproductive capacity and did not promote significant DNA damage in those cells. In conclusion, the β-TCP-MCPM cement associated with mesoporous silica might be considered as a potential bone substitute for the repair and regeneration of bone defects.
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spelling Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluationbioactivitybone repaircalcium phosphate cementinjectabilitymesoporous silicatoxicity analysisCalcium phosphate cement has been widely investigated as a bone graft substitute due to its excellent self-setting ability, biocompatibility, osteoconductivity and moldability. In addition, mesoporous materials have been studied as potential materials for application in medical devices due to their large surface area, which is capable of loading numerous biological molecules, besides being bioactive. In this study, bone β-TCP-MCPM-based injectable cement with mesoporous silica particles was synthesized and characterized in terms of its mechanical properties, microstructure, porosity, injectability, in vitro bioactivity and degradability; together with toxicity effects in CHO-K1 cell culture. The results showed that the β-TCP-MCPM cement is bioactive after soaking in simulated body fluid solution, and mesoporous silica particles provided better physicochemical properties compared with silica-free cement. Toxicity assays showed low CHO-K1 cell viability after treatment with more concentrated extracts (200 mg ml-1). However, this behavior did not compromise the reproductive capacity and did not promote significant DNA damage in those cells. In conclusion, the β-TCP-MCPM cement associated with mesoporous silica might be considered as a potential bone substitute for the repair and regeneration of bone defects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) Institute of ChemistrySão Paulo State University (Unesp) School of DentistryAzurebio S.L.Instituto de Cerámica y Vidrio CSICSão Paulo State University (Unesp) Institute of ChemistrySão Paulo State University (Unesp) School of DentistryFAPESP: 2012/21735-6Universidade Estadual Paulista (Unesp)Azurebio S.L.CSICMendes, L. S. [UNESP]Saska, S. [UNESP]Coelho, F. [UNESP]Capote, T S De O [UNESP]Scarel-Caminaga, R. M. [UNESP]Marchetto, R. [UNESP]Carrodeguas, R. G.Gaspar, A. M.M. [UNESP]Rodríguez, M. A.2018-12-11T16:52:09Z2018-12-11T16:52:09Z2018-02-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1088/1748-605X/aa9085Biomedical Materials (Bristol), v. 13, n. 2, 2018.1748-605X1748-6041http://hdl.handle.net/11449/17072210.1088/1748-605X/aa90852-s2.0-850426627482-s2.0-85042662748.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedical Materials (Bristol)0,768info:eu-repo/semantics/openAccess2023-12-04T06:16:03Zoai:repositorio.unesp.br:11449/170722Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-04T06:16:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
title Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
spellingShingle Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
Mendes, L. S. [UNESP]
bioactivity
bone repair
calcium phosphate cement
injectability
mesoporous silica
toxicity analysis
title_short Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
title_full Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
title_fullStr Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
title_full_unstemmed Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
title_sort Injectable β-TCP/MCPM cement associated with mesoporous silica for bone regeneration: Characterization and toxicity evaluation
author Mendes, L. S. [UNESP]
author_facet Mendes, L. S. [UNESP]
Saska, S. [UNESP]
Coelho, F. [UNESP]
Capote, T S De O [UNESP]
Scarel-Caminaga, R. M. [UNESP]
Marchetto, R. [UNESP]
Carrodeguas, R. G.
Gaspar, A. M.M. [UNESP]
Rodríguez, M. A.
author_role author
author2 Saska, S. [UNESP]
Coelho, F. [UNESP]
Capote, T S De O [UNESP]
Scarel-Caminaga, R. M. [UNESP]
Marchetto, R. [UNESP]
Carrodeguas, R. G.
Gaspar, A. M.M. [UNESP]
Rodríguez, M. A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Azurebio S.L.
CSIC
dc.contributor.author.fl_str_mv Mendes, L. S. [UNESP]
Saska, S. [UNESP]
Coelho, F. [UNESP]
Capote, T S De O [UNESP]
Scarel-Caminaga, R. M. [UNESP]
Marchetto, R. [UNESP]
Carrodeguas, R. G.
Gaspar, A. M.M. [UNESP]
Rodríguez, M. A.
dc.subject.por.fl_str_mv bioactivity
bone repair
calcium phosphate cement
injectability
mesoporous silica
toxicity analysis
topic bioactivity
bone repair
calcium phosphate cement
injectability
mesoporous silica
toxicity analysis
description Calcium phosphate cement has been widely investigated as a bone graft substitute due to its excellent self-setting ability, biocompatibility, osteoconductivity and moldability. In addition, mesoporous materials have been studied as potential materials for application in medical devices due to their large surface area, which is capable of loading numerous biological molecules, besides being bioactive. In this study, bone β-TCP-MCPM-based injectable cement with mesoporous silica particles was synthesized and characterized in terms of its mechanical properties, microstructure, porosity, injectability, in vitro bioactivity and degradability; together with toxicity effects in CHO-K1 cell culture. The results showed that the β-TCP-MCPM cement is bioactive after soaking in simulated body fluid solution, and mesoporous silica particles provided better physicochemical properties compared with silica-free cement. Toxicity assays showed low CHO-K1 cell viability after treatment with more concentrated extracts (200 mg ml-1). However, this behavior did not compromise the reproductive capacity and did not promote significant DNA damage in those cells. In conclusion, the β-TCP-MCPM cement associated with mesoporous silica might be considered as a potential bone substitute for the repair and regeneration of bone defects.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:52:09Z
2018-12-11T16:52:09Z
2018-02-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1088/1748-605X/aa9085
Biomedical Materials (Bristol), v. 13, n. 2, 2018.
1748-605X
1748-6041
http://hdl.handle.net/11449/170722
10.1088/1748-605X/aa9085
2-s2.0-85042662748
2-s2.0-85042662748.pdf
url http://dx.doi.org/10.1088/1748-605X/aa9085
http://hdl.handle.net/11449/170722
identifier_str_mv Biomedical Materials (Bristol), v. 13, n. 2, 2018.
1748-605X
1748-6041
10.1088/1748-605X/aa9085
2-s2.0-85042662748
2-s2.0-85042662748.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedical Materials (Bristol)
0,768
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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