Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992010000400012 http://hdl.handle.net/11449/226164 |
Resumo: | Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW®(Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi. © CEVAP 2010. |
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Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venomAntimicrobial activityBothrops leucurusL-amino oxidaseSome snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW®(Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi. © CEVAP 2010.Department of Clinical and Toxicological Analysis School of Pharmacy Federal University of Ceará, Fortaleza, Ceará StateDepartment of Physiology and Pharmacology Federal University of Ceará, Fortaleza, Ceará StateLaboratory of Cell Biology and Chemistry for Proteins and Peptides São Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo StateDepartment of Biochemistry Institute of Biology State University of Campinas UNICAMP Campinas, São Paulo StateDepartment of Molecular Biology Center of Exact Sciences and Nature Federal University of Paraíba, João Pessoa, Paraíba StateLaboratory of Cell Biology and Chemistry for Proteins and Peptides São Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo StateFederal University of CearáUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Federal University of ParaíbaTorres, A. F.C.Dantas, R. T.Menezes, R. R.P.P.B.Toyama, M. H. [UNESP]Filho, E. D.Oliveira, M. F.Nogueira, N. A.P.Oliveira, M. R.Monteiro, H. S.A.Martins, A. M.C.2022-04-28T21:39:41Z2022-04-28T21:39:41Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article614-622http://dx.doi.org/10.1590/S1678-91992010000400012Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 4, p. 614-622, 2010.1678-9199http://hdl.handle.net/11449/22616410.1590/S1678-919920100004000122-s2.0-78650332067Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T21:39:41Zoai:repositorio.unesp.br:11449/226164Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:07:59.621171Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
title |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
spellingShingle |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom Torres, A. F.C. Antimicrobial activity Bothrops leucurus L-amino oxidase |
title_short |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
title_full |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
title_fullStr |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
title_full_unstemmed |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
title_sort |
Antimicrobial activity of an l-amino acid oxidase isolated from bothrops leucurus snake venom |
author |
Torres, A. F.C. |
author_facet |
Torres, A. F.C. Dantas, R. T. Menezes, R. R.P.P.B. Toyama, M. H. [UNESP] Filho, E. D. Oliveira, M. F. Nogueira, N. A.P. Oliveira, M. R. Monteiro, H. S.A. Martins, A. M.C. |
author_role |
author |
author2 |
Dantas, R. T. Menezes, R. R.P.P.B. Toyama, M. H. [UNESP] Filho, E. D. Oliveira, M. F. Nogueira, N. A.P. Oliveira, M. R. Monteiro, H. S.A. Martins, A. M.C. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Ceará Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) Federal University of Paraíba |
dc.contributor.author.fl_str_mv |
Torres, A. F.C. Dantas, R. T. Menezes, R. R.P.P.B. Toyama, M. H. [UNESP] Filho, E. D. Oliveira, M. F. Nogueira, N. A.P. Oliveira, M. R. Monteiro, H. S.A. Martins, A. M.C. |
dc.subject.por.fl_str_mv |
Antimicrobial activity Bothrops leucurus L-amino oxidase |
topic |
Antimicrobial activity Bothrops leucurus L-amino oxidase |
description |
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW®(Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi. © CEVAP 2010. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2022-04-28T21:39:41Z 2022-04-28T21:39:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992010000400012 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 4, p. 614-622, 2010. 1678-9199 http://hdl.handle.net/11449/226164 10.1590/S1678-91992010000400012 2-s2.0-78650332067 |
url |
http://dx.doi.org/10.1590/S1678-91992010000400012 http://hdl.handle.net/11449/226164 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 4, p. 614-622, 2010. 1678-9199 10.1590/S1678-91992010000400012 2-s2.0-78650332067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
614-622 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128899127705600 |