Lignin surface area influenced by biomass heterogeneity and pretreatment process
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s13399-021-02264-5 http://hdl.handle.net/11449/230161 |
Resumo: | Biomass conversion is challenged by the heterogeneity and lignin, a component that acts as a barrier to the enzymatic hydrolysis of polysaccharides. This study evaluated the effect of pretreatments on different fractions of sugarcane biomass lignin and cellulose exposure. Two different methods were used: dilute sulfuric acid (5%, 10%, and 20% w/w at 121 °C/30 min) and partial delignification with sodium chlorite (30% m/m at 70 °C/1 h) to reduce the exposed surface of lignin. The surface area of lignin was obtained through the cationic dye Azure B, and cellulose access by enzymatic hydrolysis (15 FPU/g for 24 h—Cellic Cetec 2—Novozymes) was employed. Acid pretreatment was efficient in hemicellulose removal, and delignification removed lignin from all the biomasses fractions. Azure B dyes determined the surface area filled with lignin after dilute sulfuric acid pretreatment and partial delignification. The sample with the largest total surface area of lignin (52 m2/g of material) was the leaf pretreated with 5% sulfuric acid. The external fraction presented the largest specific surface area (240 m2/g) after partial delignification. These results indicate that reducing the lignin surface area enhances the glucose yield of enzymatic hydrolysis. |
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Repositório Institucional da UNESP |
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Lignin surface area influenced by biomass heterogeneity and pretreatment processAzure BCellulose accessibilityDiluted acidSugarcane biomassXyloseBiomass conversion is challenged by the heterogeneity and lignin, a component that acts as a barrier to the enzymatic hydrolysis of polysaccharides. This study evaluated the effect of pretreatments on different fractions of sugarcane biomass lignin and cellulose exposure. Two different methods were used: dilute sulfuric acid (5%, 10%, and 20% w/w at 121 °C/30 min) and partial delignification with sodium chlorite (30% m/m at 70 °C/1 h) to reduce the exposed surface of lignin. The surface area of lignin was obtained through the cationic dye Azure B, and cellulose access by enzymatic hydrolysis (15 FPU/g for 24 h—Cellic Cetec 2—Novozymes) was employed. Acid pretreatment was efficient in hemicellulose removal, and delignification removed lignin from all the biomasses fractions. Azure B dyes determined the surface area filled with lignin after dilute sulfuric acid pretreatment and partial delignification. The sample with the largest total surface area of lignin (52 m2/g of material) was the leaf pretreated with 5% sulfuric acid. The external fraction presented the largest specific surface area (240 m2/g) after partial delignification. These results indicate that reducing the lignin surface area enhances the glucose yield of enzymatic hydrolysis.Institute for Research in Bioenergy (IPBEN) University of São Paulo State (UNESP), SPBiochemistry and Microbiology Department University of São Paulo State (UNESP), SPInstitute for Research in Bioenergy (IPBEN) University of São Paulo State (UNESP), SPBiochemistry and Microbiology Department University of São Paulo State (UNESP), SPUniversidade Estadual Paulista (UNESP)de Azevedo, Gabriel Oliveira [UNESP]Shimizu, Felipe Lange [UNESP]Fontes, Luciana Coelho [UNESP]Contiero, Jonas [UNESP]Brienzo, Michel [UNESP]2022-04-29T08:38:12Z2022-04-29T08:38:12Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s13399-021-02264-5Biomass Conversion and Biorefinery.2190-68232190-6815http://hdl.handle.net/11449/23016110.1007/s13399-021-02264-52-s2.0-85122251463Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomass Conversion and Biorefineryinfo:eu-repo/semantics/openAccess2022-04-29T08:38:12Zoai:repositorio.unesp.br:11449/230161Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:55:02.793428Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
title |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
spellingShingle |
Lignin surface area influenced by biomass heterogeneity and pretreatment process de Azevedo, Gabriel Oliveira [UNESP] Azure B Cellulose accessibility Diluted acid Sugarcane biomass Xylose |
title_short |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
title_full |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
title_fullStr |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
title_full_unstemmed |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
title_sort |
Lignin surface area influenced by biomass heterogeneity and pretreatment process |
author |
de Azevedo, Gabriel Oliveira [UNESP] |
author_facet |
de Azevedo, Gabriel Oliveira [UNESP] Shimizu, Felipe Lange [UNESP] Fontes, Luciana Coelho [UNESP] Contiero, Jonas [UNESP] Brienzo, Michel [UNESP] |
author_role |
author |
author2 |
Shimizu, Felipe Lange [UNESP] Fontes, Luciana Coelho [UNESP] Contiero, Jonas [UNESP] Brienzo, Michel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Azevedo, Gabriel Oliveira [UNESP] Shimizu, Felipe Lange [UNESP] Fontes, Luciana Coelho [UNESP] Contiero, Jonas [UNESP] Brienzo, Michel [UNESP] |
dc.subject.por.fl_str_mv |
Azure B Cellulose accessibility Diluted acid Sugarcane biomass Xylose |
topic |
Azure B Cellulose accessibility Diluted acid Sugarcane biomass Xylose |
description |
Biomass conversion is challenged by the heterogeneity and lignin, a component that acts as a barrier to the enzymatic hydrolysis of polysaccharides. This study evaluated the effect of pretreatments on different fractions of sugarcane biomass lignin and cellulose exposure. Two different methods were used: dilute sulfuric acid (5%, 10%, and 20% w/w at 121 °C/30 min) and partial delignification with sodium chlorite (30% m/m at 70 °C/1 h) to reduce the exposed surface of lignin. The surface area of lignin was obtained through the cationic dye Azure B, and cellulose access by enzymatic hydrolysis (15 FPU/g for 24 h—Cellic Cetec 2—Novozymes) was employed. Acid pretreatment was efficient in hemicellulose removal, and delignification removed lignin from all the biomasses fractions. Azure B dyes determined the surface area filled with lignin after dilute sulfuric acid pretreatment and partial delignification. The sample with the largest total surface area of lignin (52 m2/g of material) was the leaf pretreated with 5% sulfuric acid. The external fraction presented the largest specific surface area (240 m2/g) after partial delignification. These results indicate that reducing the lignin surface area enhances the glucose yield of enzymatic hydrolysis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:12Z 2022-04-29T08:38:12Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s13399-021-02264-5 Biomass Conversion and Biorefinery. 2190-6823 2190-6815 http://hdl.handle.net/11449/230161 10.1007/s13399-021-02264-5 2-s2.0-85122251463 |
url |
http://dx.doi.org/10.1007/s13399-021-02264-5 http://hdl.handle.net/11449/230161 |
identifier_str_mv |
Biomass Conversion and Biorefinery. 2190-6823 2190-6815 10.1007/s13399-021-02264-5 2-s2.0-85122251463 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomass Conversion and Biorefinery |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128720455598080 |