Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-8478cr20141539 http://hdl.handle.net/11449/168201 |
Resumo: | The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of producing enterotoxins. It was confirmed the genetic similarity among Bacillus cereus isolates from raw, pasteurized and UHT milk, therefore demonstrating that the microorganism is able to withstand UHT treatment. These results should serve as a warning to health authorities, given that 13.8% of samples were not in accordance with standards established by the Department of Health for containing a potentially pathogen agent, therefore indicating that contamination of milk by sporulating bacteria should be avoided. |
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Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimeraseDetection of bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic dna polymerase chain reactionBacillus cereusEnterotoxinsMicrobiological qualityPasteurized milkRaw milkUHT milkThe present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of producing enterotoxins. It was confirmed the genetic similarity among Bacillus cereus isolates from raw, pasteurized and UHT milk, therefore demonstrating that the microorganism is able to withstand UHT treatment. These results should serve as a warning to health authorities, given that 13.8% of samples were not in accordance with standards established by the Department of Health for containing a potentially pathogen agent, therefore indicating that contamination of milk by sporulating bacteria should be avoided.Departamento de Medicina Veterinária Universidade de São Paulo (USP), Av. Duque de Caxias Norte 225Departamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nCentro Universitário de Rio Preto (UNIRP)Departamento de Zootecnia FZEA USPDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Centro Universitário de Rio Preto (UNIRP)Vidal, Ana Maria CentolaJunior, Oswaldo Durival Rossi [UNESP]de Abreu, Irlan LeiteBürger, Karina Paes [UNESP]Cardoso, Marita Vedovelli [UNESP]Gonçalves, Ana Carolina Siqueira [UNESP]Rossi, Gabriel Augusto Marques [UNESP]D’Abreu, Léa Furlan2018-12-11T16:40:12Z2018-12-11T16:40:12Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article286-292application/pdfhttp://dx.doi.org/10.1590/0103-8478cr20141539Ciencia Rural, v. 46, n. 2, p. 286-292, 2016.1678-45960103-8478http://hdl.handle.net/11449/16820110.1590/0103-8478cr20141539S0103-847820160002002862-s2.0-84949765827S0103-84782016000200286.pdf60481366783932660000-0003-1187-198XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2023-11-24T06:14:51Zoai:repositorio.unesp.br:11449/168201Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-24T06:14:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase Detection of bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic dna polymerase chain reaction |
title |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
spellingShingle |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase Vidal, Ana Maria Centola Bacillus cereus Enterotoxins Microbiological quality Pasteurized milk Raw milk UHT milk |
title_short |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
title_full |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
title_fullStr |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
title_full_unstemmed |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
title_sort |
Detecção de Bacillus cereus isolado durante o fluxograma de produção do leite tratado por ultra alta temperatura através do DNA polimórfico amplificado ao acaso através da reação em cadeia pela polimerase |
author |
Vidal, Ana Maria Centola |
author_facet |
Vidal, Ana Maria Centola Junior, Oswaldo Durival Rossi [UNESP] de Abreu, Irlan Leite Bürger, Karina Paes [UNESP] Cardoso, Marita Vedovelli [UNESP] Gonçalves, Ana Carolina Siqueira [UNESP] Rossi, Gabriel Augusto Marques [UNESP] D’Abreu, Léa Furlan |
author_role |
author |
author2 |
Junior, Oswaldo Durival Rossi [UNESP] de Abreu, Irlan Leite Bürger, Karina Paes [UNESP] Cardoso, Marita Vedovelli [UNESP] Gonçalves, Ana Carolina Siqueira [UNESP] Rossi, Gabriel Augusto Marques [UNESP] D’Abreu, Léa Furlan |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Centro Universitário de Rio Preto (UNIRP) |
dc.contributor.author.fl_str_mv |
Vidal, Ana Maria Centola Junior, Oswaldo Durival Rossi [UNESP] de Abreu, Irlan Leite Bürger, Karina Paes [UNESP] Cardoso, Marita Vedovelli [UNESP] Gonçalves, Ana Carolina Siqueira [UNESP] Rossi, Gabriel Augusto Marques [UNESP] D’Abreu, Léa Furlan |
dc.subject.por.fl_str_mv |
Bacillus cereus Enterotoxins Microbiological quality Pasteurized milk Raw milk UHT milk |
topic |
Bacillus cereus Enterotoxins Microbiological quality Pasteurized milk Raw milk UHT milk |
description |
The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of producing enterotoxins. It was confirmed the genetic similarity among Bacillus cereus isolates from raw, pasteurized and UHT milk, therefore demonstrating that the microorganism is able to withstand UHT treatment. These results should serve as a warning to health authorities, given that 13.8% of samples were not in accordance with standards established by the Department of Health for containing a potentially pathogen agent, therefore indicating that contamination of milk by sporulating bacteria should be avoided. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 2018-12-11T16:40:12Z 2018-12-11T16:40:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-8478cr20141539 Ciencia Rural, v. 46, n. 2, p. 286-292, 2016. 1678-4596 0103-8478 http://hdl.handle.net/11449/168201 10.1590/0103-8478cr20141539 S0103-84782016000200286 2-s2.0-84949765827 S0103-84782016000200286.pdf 6048136678393266 0000-0003-1187-198X |
url |
http://dx.doi.org/10.1590/0103-8478cr20141539 http://hdl.handle.net/11449/168201 |
identifier_str_mv |
Ciencia Rural, v. 46, n. 2, p. 286-292, 2016. 1678-4596 0103-8478 10.1590/0103-8478cr20141539 S0103-84782016000200286 2-s2.0-84949765827 S0103-84782016000200286.pdf 6048136678393266 0000-0003-1187-198X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Rural 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
286-292 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046636766625792 |