Efeito probiótico do Bacillus amyloliquefaciens na aerocistite aguda em tilápias-do-Nilo (Oreochromis niloticus)

• Autor(a) principal: Petrillo, Thalita Regina [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/154734; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-03-2017/000881020.pdf
• topic: Probiotics; Probióticos; Bactérias; Inflamação; Teleosteos; Peixe; Tilápia-do-Nilo

The use of probiotics arises as an alternative to the use of antibiotics in fishes, preventing them from becoming sick. The scope of this study was to evaluate the effects of food with added probiotic containing Bacillus amyloliquefaciens in acute inflammation against A.hydrophila in the Nile-tilapia (Oreochromis niloticus). To that end, there were used 936 copies of the Nile-tilapia (30g), evenly distributed in 12 net tanks with 78 fish in each. The fishes were fed for 60 days with food added with the probiotic bacteria in different concentrations, with the following groups being formed; Group 1 (control) fed with basal food without added probiotic: Group 2 (T1) supplemented with 0.75 grams of probiotic / kg food; Group 3 (T2) supplemented with 1.5 grams of the probiotic / kg food; Group 4 (T3) supplemented with 3 grams of probiotic / kg food. For the inflammatory stimulus it was inoculated in the swimming bladder of fish 0.5 ml of saline solution 0.65% in the control group and 0.5 ml x106 of a solution containing inactivated A. hydrophila in the other groups. Blood collections and inflammatory exudate were held in the periods of 12, 24 and 48 hours after the inflammatory stimulus (HPE), for the following analysis: haematological variables, basal glucose levels, respiratory activity of sanguine leukocytes (oxidative burst), qualitative and quantitative characteristics of the exudate, lytic activity of serum proteases and agglutination bacterial activity. The results were compared using analysis of variance (ANOVA) at 5% probability and the difference between the means was compared by Tukey test. There were no significant hematological changes between the groups of Nile-tilapia treated and stimulated and the control group, the same thing can be observed in glycemic indeces and respiratory activity of blood leucocytes. The inflammatory exudate was characterized by the predominant accumulation ...