Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.33263/BRIAC113.1008910098 http://hdl.handle.net/11449/206849 |
Resumo: | The development of biomaterials for the regeneration of tissues is of great importance, and their demand increases every day, due to the increase of the aging population, the expectation, and quality of life, as well as the increase of the accident rates (traffic and violence). Scaffolds are a three-dimensional structure designed to withstand cellular infiltration, growth, and differentiation in order to improve the development and formation of new tissues. Many biomaterials can be used to make these structures, such as bioceramics and biopolymers. However, few studies have been conducted to evaluate its microbiological contamination and the influence of sterilization methods may have on the structure and properties. The objective of this work was to evaluate different sterilization processes and their influence on the physical-chemical properties of Scaffolds made with calcium phosphate and bacterial cellulose. Three sterilization techniques (steam sterilization, ultraviolet radiation sterilization, and microwave sterilization) were evaluated. The results showed that steam sterilization was effective in eliminating microorganisms. Microwave sterilization significantly reduced the amount of CFU / mL, while ultraviolet radiation sterilization did not show efficacy. Changes in the physical-chemical properties of the Scaffolds evaluated after sterilization was not observed, except a slight darkening in the samples treated by steam sterilization or treated by microwave sterilization due to the presence of sodium alginate in the formulation. It was concluded that the steam sterilization process was the most effective. |
id |
UNSP_80ad9d4478ec83cfe4a2c367a008e78d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/206849 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regenerationBacterial celluloseCalcium phosphatesScaffoldsStatistical analysisSterilization processThe development of biomaterials for the regeneration of tissues is of great importance, and their demand increases every day, due to the increase of the aging population, the expectation, and quality of life, as well as the increase of the accident rates (traffic and violence). Scaffolds are a three-dimensional structure designed to withstand cellular infiltration, growth, and differentiation in order to improve the development and formation of new tissues. Many biomaterials can be used to make these structures, such as bioceramics and biopolymers. However, few studies have been conducted to evaluate its microbiological contamination and the influence of sterilization methods may have on the structure and properties. The objective of this work was to evaluate different sterilization processes and their influence on the physical-chemical properties of Scaffolds made with calcium phosphate and bacterial cellulose. Three sterilization techniques (steam sterilization, ultraviolet radiation sterilization, and microwave sterilization) were evaluated. The results showed that steam sterilization was effective in eliminating microorganisms. Microwave sterilization significantly reduced the amount of CFU / mL, while ultraviolet radiation sterilization did not show efficacy. Changes in the physical-chemical properties of the Scaffolds evaluated after sterilization was not observed, except a slight darkening in the samples treated by steam sterilization or treated by microwave sterilization due to the presence of sodium alginate in the formulation. It was concluded that the steam sterilization process was the most effective.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physical Chemistry Institute of Chemistry São Paulo State University (UNESP)Department of Dental Materials and Prostheses Araraquara Dental School São Paulo State University (UNESP)Center of Engineering and Chemical ResearchMathematics Department Science Faculty National University of SaltaInnovatec's-Biotechnology Research and DevelopmentDepartment of Physical Chemistry Institute of Chemistry São Paulo State University (UNESP)Department of Dental Materials and Prostheses Araraquara Dental School São Paulo State University (UNESP)FAPESP: 2017 / 15487-3Universidade Estadual Paulista (Unesp)Center of Engineering and Chemical ResearchNational University of SaltaInnovatec's-Biotechnology Research and DevelopmentFrancisco, Erick Mark [UNESP]Zoccolotti, Jacqueline de Oliveira [UNESP]Tiomnova, Olinka TiomnoTolaba, Angel GustavoChanfrau, Jorge Enrique Rodriguez [UNESP]Jorge, Janaina Habib [UNESP]Basmaji, PierreGuastaldi, Antonio Carlos [UNESP]2021-06-25T10:44:52Z2021-06-25T10:44:52Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10089-10098http://dx.doi.org/10.33263/BRIAC113.1008910098Biointerface Research in Applied Chemistry, v. 11, n. 3, p. 10089-10098, 2021.2069-5837http://hdl.handle.net/11449/20684910.33263/BRIAC113.10089100982-s2.0-85096289945Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiointerface Research in Applied Chemistryinfo:eu-repo/semantics/openAccess2021-10-23T15:25:37Zoai:repositorio.unesp.br:11449/206849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:46:43.889830Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
title |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
spellingShingle |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration Francisco, Erick Mark [UNESP] Bacterial cellulose Calcium phosphates Scaffolds Statistical analysis Sterilization process |
title_short |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
title_full |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
title_fullStr |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
title_full_unstemmed |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
title_sort |
Sterilization of scaffolds of calcium phosphates and bacterial cellulose for their use in tissue regeneration |
author |
Francisco, Erick Mark [UNESP] |
author_facet |
Francisco, Erick Mark [UNESP] Zoccolotti, Jacqueline de Oliveira [UNESP] Tiomnova, Olinka Tiomno Tolaba, Angel Gustavo Chanfrau, Jorge Enrique Rodriguez [UNESP] Jorge, Janaina Habib [UNESP] Basmaji, Pierre Guastaldi, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Zoccolotti, Jacqueline de Oliveira [UNESP] Tiomnova, Olinka Tiomno Tolaba, Angel Gustavo Chanfrau, Jorge Enrique Rodriguez [UNESP] Jorge, Janaina Habib [UNESP] Basmaji, Pierre Guastaldi, Antonio Carlos [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Center of Engineering and Chemical Research National University of Salta Innovatec's-Biotechnology Research and Development |
dc.contributor.author.fl_str_mv |
Francisco, Erick Mark [UNESP] Zoccolotti, Jacqueline de Oliveira [UNESP] Tiomnova, Olinka Tiomno Tolaba, Angel Gustavo Chanfrau, Jorge Enrique Rodriguez [UNESP] Jorge, Janaina Habib [UNESP] Basmaji, Pierre Guastaldi, Antonio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Bacterial cellulose Calcium phosphates Scaffolds Statistical analysis Sterilization process |
topic |
Bacterial cellulose Calcium phosphates Scaffolds Statistical analysis Sterilization process |
description |
The development of biomaterials for the regeneration of tissues is of great importance, and their demand increases every day, due to the increase of the aging population, the expectation, and quality of life, as well as the increase of the accident rates (traffic and violence). Scaffolds are a three-dimensional structure designed to withstand cellular infiltration, growth, and differentiation in order to improve the development and formation of new tissues. Many biomaterials can be used to make these structures, such as bioceramics and biopolymers. However, few studies have been conducted to evaluate its microbiological contamination and the influence of sterilization methods may have on the structure and properties. The objective of this work was to evaluate different sterilization processes and their influence on the physical-chemical properties of Scaffolds made with calcium phosphate and bacterial cellulose. Three sterilization techniques (steam sterilization, ultraviolet radiation sterilization, and microwave sterilization) were evaluated. The results showed that steam sterilization was effective in eliminating microorganisms. Microwave sterilization significantly reduced the amount of CFU / mL, while ultraviolet radiation sterilization did not show efficacy. Changes in the physical-chemical properties of the Scaffolds evaluated after sterilization was not observed, except a slight darkening in the samples treated by steam sterilization or treated by microwave sterilization due to the presence of sodium alginate in the formulation. It was concluded that the steam sterilization process was the most effective. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:44:52Z 2021-06-25T10:44:52Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.33263/BRIAC113.1008910098 Biointerface Research in Applied Chemistry, v. 11, n. 3, p. 10089-10098, 2021. 2069-5837 http://hdl.handle.net/11449/206849 10.33263/BRIAC113.1008910098 2-s2.0-85096289945 |
url |
http://dx.doi.org/10.33263/BRIAC113.1008910098 http://hdl.handle.net/11449/206849 |
identifier_str_mv |
Biointerface Research in Applied Chemistry, v. 11, n. 3, p. 10089-10098, 2021. 2069-5837 10.33263/BRIAC113.1008910098 2-s2.0-85096289945 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biointerface Research in Applied Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10089-10098 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128857107070976 |