Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation

Detalhes bibliográficos
Autor(a) principal: Cerri, Paulo Sérgio [UNESP]
Data de Publicação: 2010
Outros Autores: Pereira-Junior, Jorge Alonso, Biselli, Natalia Barrionuevo, Cerri, Estela Sasso [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/j.1469-7580.2010.01249.x
http://hdl.handle.net/11449/16606
Resumo: During the active tooth eruption process, structural changes in the lamina propria are necessary to provide extracellular matrix remodelling and for the establishment of the eruptive pathway. A large number of resident cells, recruited cells and proteases have been demonstrated in the eruptive process, but the participation of MMP-9 and mast cells has not yet been demonstrated. In this study, we set out to evaluate the intensity of MMP-9 immunoexpression, the frequency of mast cells and the correlation between the incidence of mast cells and bone resorption in different phases of tooth eruption. Fragments of maxilla containing first molars, obtained from 9-, 11-, 13- and 16-day-old rats, were fixed in 4% formaldehyde, decalcified and embedded in paraffin. Sagittal sections were stained with Masson's trichrome or submitted to the tartrate-resistant acid phosphatase method for quantification of osteoclasts. Sections stained by 1% toluidine blue were used for quantification of metachromatic mast cells mm-2 of lamina propria. The expression of MMP-9 in the lamina propria was evaluated by immunohistochemistry. In the 9-day-old rats, the lamina propria contained few mast cells and occasional osteoclasts were found in the bone surface overlying the occlusal portion of the tooth germs. Otherwise, a significant increase in the number of mast cells was observed in the intra-osseous phase of tooth eruption (11-day-old rats), period in which numerous TRAP-positive osteoclasts were found in the bone surface. MMP-9 immunolabelling was detected in fibroblasts, mast cells and macrophage-like cells of the lamina propria in all ages studied. However, an enhanced immunolabelling was evident in the advanced phase of tooth eruption (16-day-old rats). During the intra-osseous phase, the parallel between the high frequency of both mast cells and osteoclasts suggests that mast cells could exert a paracrine function on the osteoclasts and then stimulate bone resorption. The immunoexpression of MMP-9 in different cells of lamina propria, including mast cells, indicates that this enzyme participates in the degradation of ECM, mainly during late phase of mucosal penetration. Thus mast cells and MMP-9 are involved in the complex process of degradation of the eruptive pathway extracellular matrix.
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spelling Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluationeruptive pathwayimmunohistochemistrylamina propriamast cellsmetalloproteinase-9oral mucosaDuring the active tooth eruption process, structural changes in the lamina propria are necessary to provide extracellular matrix remodelling and for the establishment of the eruptive pathway. A large number of resident cells, recruited cells and proteases have been demonstrated in the eruptive process, but the participation of MMP-9 and mast cells has not yet been demonstrated. In this study, we set out to evaluate the intensity of MMP-9 immunoexpression, the frequency of mast cells and the correlation between the incidence of mast cells and bone resorption in different phases of tooth eruption. Fragments of maxilla containing first molars, obtained from 9-, 11-, 13- and 16-day-old rats, were fixed in 4% formaldehyde, decalcified and embedded in paraffin. Sagittal sections were stained with Masson's trichrome or submitted to the tartrate-resistant acid phosphatase method for quantification of osteoclasts. Sections stained by 1% toluidine blue were used for quantification of metachromatic mast cells mm-2 of lamina propria. The expression of MMP-9 in the lamina propria was evaluated by immunohistochemistry. In the 9-day-old rats, the lamina propria contained few mast cells and occasional osteoclasts were found in the bone surface overlying the occlusal portion of the tooth germs. Otherwise, a significant increase in the number of mast cells was observed in the intra-osseous phase of tooth eruption (11-day-old rats), period in which numerous TRAP-positive osteoclasts were found in the bone surface. MMP-9 immunolabelling was detected in fibroblasts, mast cells and macrophage-like cells of the lamina propria in all ages studied. However, an enhanced immunolabelling was evident in the advanced phase of tooth eruption (16-day-old rats). During the intra-osseous phase, the parallel between the high frequency of both mast cells and osteoclasts suggests that mast cells could exert a paracrine function on the osteoclasts and then stimulate bone resorption. The immunoexpression of MMP-9 in different cells of lamina propria, including mast cells, indicates that this enzyme participates in the degradation of ECM, mainly during late phase of mucosal penetration. Thus mast cells and MMP-9 are involved in the complex process of degradation of the eruptive pathway extracellular matrix.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, São Paulo State Univ, Dept Morphol, Lab Histol & Embryol,Dent Sch, BR-14801903 São Paulo, BrazilUniv Estadual Paulista, São Paulo State Univ, Dept Morphol, Lab Histol & Embryol,Dent Sch, BR-14801903 São Paulo, BrazilCNPq: 307692/2006-0FAPESP: 04/09898-0Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Cerri, Paulo Sérgio [UNESP]Pereira-Junior, Jorge AlonsoBiselli, Natalia BarrionuevoCerri, Estela Sasso [UNESP]2014-05-20T13:46:50Z2014-05-20T13:46:50Z2010-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article116-125http://dx.doi.org/10.1111/j.1469-7580.2010.01249.xJournal of Anatomy. Malden: Wiley-blackwell, v. 217, n. 2, p. 116-125, 2010.0021-8782http://hdl.handle.net/11449/1660610.1111/j.1469-7580.2010.01249.xWOS:000279736300004327849591120788244556300768413020000-0001-5756-5828Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Anatomy2.4791,078info:eu-repo/semantics/openAccess2024-09-27T15:15:10Zoai:repositorio.unesp.br:11449/16606Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
title Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
spellingShingle Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
Cerri, Paulo Sérgio [UNESP]
eruptive pathway
immunohistochemistry
lamina propria
mast cells
metalloproteinase-9
oral mucosa
title_short Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
title_full Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
title_fullStr Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
title_full_unstemmed Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
title_sort Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation
author Cerri, Paulo Sérgio [UNESP]
author_facet Cerri, Paulo Sérgio [UNESP]
Pereira-Junior, Jorge Alonso
Biselli, Natalia Barrionuevo
Cerri, Estela Sasso [UNESP]
author_role author
author2 Pereira-Junior, Jorge Alonso
Biselli, Natalia Barrionuevo
Cerri, Estela Sasso [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cerri, Paulo Sérgio [UNESP]
Pereira-Junior, Jorge Alonso
Biselli, Natalia Barrionuevo
Cerri, Estela Sasso [UNESP]
dc.subject.por.fl_str_mv eruptive pathway
immunohistochemistry
lamina propria
mast cells
metalloproteinase-9
oral mucosa
topic eruptive pathway
immunohistochemistry
lamina propria
mast cells
metalloproteinase-9
oral mucosa
description During the active tooth eruption process, structural changes in the lamina propria are necessary to provide extracellular matrix remodelling and for the establishment of the eruptive pathway. A large number of resident cells, recruited cells and proteases have been demonstrated in the eruptive process, but the participation of MMP-9 and mast cells has not yet been demonstrated. In this study, we set out to evaluate the intensity of MMP-9 immunoexpression, the frequency of mast cells and the correlation between the incidence of mast cells and bone resorption in different phases of tooth eruption. Fragments of maxilla containing first molars, obtained from 9-, 11-, 13- and 16-day-old rats, were fixed in 4% formaldehyde, decalcified and embedded in paraffin. Sagittal sections were stained with Masson's trichrome or submitted to the tartrate-resistant acid phosphatase method for quantification of osteoclasts. Sections stained by 1% toluidine blue were used for quantification of metachromatic mast cells mm-2 of lamina propria. The expression of MMP-9 in the lamina propria was evaluated by immunohistochemistry. In the 9-day-old rats, the lamina propria contained few mast cells and occasional osteoclasts were found in the bone surface overlying the occlusal portion of the tooth germs. Otherwise, a significant increase in the number of mast cells was observed in the intra-osseous phase of tooth eruption (11-day-old rats), period in which numerous TRAP-positive osteoclasts were found in the bone surface. MMP-9 immunolabelling was detected in fibroblasts, mast cells and macrophage-like cells of the lamina propria in all ages studied. However, an enhanced immunolabelling was evident in the advanced phase of tooth eruption (16-day-old rats). During the intra-osseous phase, the parallel between the high frequency of both mast cells and osteoclasts suggests that mast cells could exert a paracrine function on the osteoclasts and then stimulate bone resorption. The immunoexpression of MMP-9 in different cells of lamina propria, including mast cells, indicates that this enzyme participates in the degradation of ECM, mainly during late phase of mucosal penetration. Thus mast cells and MMP-9 are involved in the complex process of degradation of the eruptive pathway extracellular matrix.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
2014-05-20T13:46:50Z
2014-05-20T13:46:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1469-7580.2010.01249.x
Journal of Anatomy. Malden: Wiley-blackwell, v. 217, n. 2, p. 116-125, 2010.
0021-8782
http://hdl.handle.net/11449/16606
10.1111/j.1469-7580.2010.01249.x
WOS:000279736300004
3278495911207882
4455630076841302
0000-0001-5756-5828
url http://dx.doi.org/10.1111/j.1469-7580.2010.01249.x
http://hdl.handle.net/11449/16606
identifier_str_mv Journal of Anatomy. Malden: Wiley-blackwell, v. 217, n. 2, p. 116-125, 2010.
0021-8782
10.1111/j.1469-7580.2010.01249.x
WOS:000279736300004
3278495911207882
4455630076841302
0000-0001-5756-5828
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Anatomy
2.479
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 116-125
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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