Análise das alterações epiteliais e da lamina própria em mucosa palatina de ratos tratados com zoledronato

• Autor(a) principal: Rosa, Vinicius Matheus
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: https://tede2.usc.br:8443/handle/tede/461
• topic: Bisfosfonatos.; Epitélio; Lâmina própria; Mucosa Bucal; Bisphosphonates; Epithelium; Lamina Propria; Mucosa; CIENCIAS DA SAUDE::ODONTOLOGIA

The oral mucosa consists of highly proliferative squamous epithelium that under normal conditions performs its functions in a harmonic way with the underlying tissues. Bisphosphonates are synthetic, anti-resorptive drugs used for the control and treatment of diseases of bone follow-up, such as: Paget's diseases; metastatic bone tumors; osteopenias and osteoporosis. This drug acts inhibiting enzymes major for proliferation and migrating cellphone, monitor for the effects of cellular phones of hard and soft. The present death is evaluated by the effects of zoledronic acid (ZL) on the epithelium and the lamina propria of the palatal mucosa of rats chronically accompanied with zoledronic acid by intravenous route. Twenty-four male Wistar rats with a mean of 20 months, weighing about 500 grams, were used. In all groups, CT (Control) - received physiological saline 0.9% (SF) via EV, without volume of 0.1 ml; Group ZL - treated with zoledronic acid given via (EV) at a dose of 35 μg / kg every two weeks (Hokugo et al., 2010) and in four subgroups according to the number of doses: GCT-6 and GZL-6 (six doses), GCT-8 and CZL-8 (high doses). In these periods, the animals were submitted to euthanasia to allow the removal of the maxilla of staining with the aid of tests for PCNA to allow qualitative and quantitative quantitative analysis. Results do not indicate differences in cell thickness. Changes in the morphology and pattern of the basal layer, such as hypercorradant cells, increased melanocyte presence, increased keratohyaline formation in the granulomatous cells and interface of the granulosa layer with the cornea were found in the experimental group (ZL). Decrease of cells immunolabeling to PCNA+ has been found between GCT-08 to GZL-08, showing citotoxic effect of the Zoledronic Acid in this cell lineage. When it comes to tissues in the bone tissue and it is suggested to investigate, concomitantly, as changes in the underlying bone tissue.