Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)

Detalhes bibliográficos
Autor(a) principal: Fernandes, Dayanne C. [UNESP]
Data de Publicação: 2019
Outros Autores: Eto, Silas F. [UNESP], Funnicelli, Michelli I.G. [UNESP], Fernandes, Camila C. [UNESP], Charlie-Silva, Ives [UNESP], Belo, Marco A.A. [UNESP], Pizauro, João M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquaculture.2018.10.045
http://hdl.handle.net/11449/187025
Resumo: Aeromonas hydrophila (Ah) are rod-shaped, Gram-negative, motile aquatic bacteria that can infect fish and mammals, including humans. Due to the importance of this pathogen for fish farming and public health, the present study produced a specific immunoglobulin (Ig) Y by immunizing laying hens with Ah cell proteins. The antibody efficacy in detecting Ah was tested by direct immunofluorescence and indirect immunohistochemistry using tissues from infected Nile tilapia. The produced IgY exhibited specificity and affinity for membrane and cytoplasmic Ah proteins, which enabled detection of the intracellular localization of the bacteria and their proteins within the phagosomes of phagocytes and detection of extracellular bacteria colonizing host tissues. Thus, the findings offer a new perspective on the use of IgY for the diagnosis and analysis of Ah pathogenesis in Nile tilapia.
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spelling Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)AeromonosisAntibodyImmunodiagnosisSepsisTeleostAeromonas hydrophila (Ah) are rod-shaped, Gram-negative, motile aquatic bacteria that can infect fish and mammals, including humans. Due to the importance of this pathogen for fish farming and public health, the present study produced a specific immunoglobulin (Ig) Y by immunizing laying hens with Ah cell proteins. The antibody efficacy in detecting Ah was tested by direct immunofluorescence and indirect immunohistochemistry using tissues from infected Nile tilapia. The produced IgY exhibited specificity and affinity for membrane and cytoplasmic Ah proteins, which enabled detection of the intracellular localization of the bacteria and their proteins within the phagosomes of phagocytes and detection of extracellular bacteria colonizing host tissues. Thus, the findings offer a new perspective on the use of IgY for the diagnosis and analysis of Ah pathogenesis in Nile tilapia.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Institute of Chemistry Sao Paulo State University (Unesp)Department of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)Department of Preventive Veterinary School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)Institute of Chemistry Sao Paulo State University (Unesp)Department of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)Department of Preventive Veterinary School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)CNPq: 140505/2016-4CNPq: 429245/2016-5Universidade Estadual Paulista (Unesp)Fernandes, Dayanne C. [UNESP]Eto, Silas F. [UNESP]Funnicelli, Michelli I.G. [UNESP]Fernandes, Camila C. [UNESP]Charlie-Silva, Ives [UNESP]Belo, Marco A.A. [UNESP]Pizauro, João M. [UNESP]2019-10-06T15:23:08Z2019-10-06T15:23:08Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article576-585http://dx.doi.org/10.1016/j.aquaculture.2018.10.045Aquaculture, v. 500, p. 576-585.0044-8486http://hdl.handle.net/11449/18702510.1016/j.aquaculture.2018.10.0452-s2.0-85055901775Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2021-10-23T12:19:00Zoai:repositorio.unesp.br:11449/187025Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:34:03.275273Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
title Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
spellingShingle Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
Fernandes, Dayanne C. [UNESP]
Aeromonosis
Antibody
Immunodiagnosis
Sepsis
Teleost
title_short Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
title_full Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
title_fullStr Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
title_full_unstemmed Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
title_sort Immunoglobulin Y in the diagnosis of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)
author Fernandes, Dayanne C. [UNESP]
author_facet Fernandes, Dayanne C. [UNESP]
Eto, Silas F. [UNESP]
Funnicelli, Michelli I.G. [UNESP]
Fernandes, Camila C. [UNESP]
Charlie-Silva, Ives [UNESP]
Belo, Marco A.A. [UNESP]
Pizauro, João M. [UNESP]
author_role author
author2 Eto, Silas F. [UNESP]
Funnicelli, Michelli I.G. [UNESP]
Fernandes, Camila C. [UNESP]
Charlie-Silva, Ives [UNESP]
Belo, Marco A.A. [UNESP]
Pizauro, João M. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fernandes, Dayanne C. [UNESP]
Eto, Silas F. [UNESP]
Funnicelli, Michelli I.G. [UNESP]
Fernandes, Camila C. [UNESP]
Charlie-Silva, Ives [UNESP]
Belo, Marco A.A. [UNESP]
Pizauro, João M. [UNESP]
dc.subject.por.fl_str_mv Aeromonosis
Antibody
Immunodiagnosis
Sepsis
Teleost
topic Aeromonosis
Antibody
Immunodiagnosis
Sepsis
Teleost
description Aeromonas hydrophila (Ah) are rod-shaped, Gram-negative, motile aquatic bacteria that can infect fish and mammals, including humans. Due to the importance of this pathogen for fish farming and public health, the present study produced a specific immunoglobulin (Ig) Y by immunizing laying hens with Ah cell proteins. The antibody efficacy in detecting Ah was tested by direct immunofluorescence and indirect immunohistochemistry using tissues from infected Nile tilapia. The produced IgY exhibited specificity and affinity for membrane and cytoplasmic Ah proteins, which enabled detection of the intracellular localization of the bacteria and their proteins within the phagosomes of phagocytes and detection of extracellular bacteria colonizing host tissues. Thus, the findings offer a new perspective on the use of IgY for the diagnosis and analysis of Ah pathogenesis in Nile tilapia.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:23:08Z
2019-10-06T15:23:08Z
2019-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2018.10.045
Aquaculture, v. 500, p. 576-585.
0044-8486
http://hdl.handle.net/11449/187025
10.1016/j.aquaculture.2018.10.045
2-s2.0-85055901775
url http://dx.doi.org/10.1016/j.aquaculture.2018.10.045
http://hdl.handle.net/11449/187025
identifier_str_mv Aquaculture, v. 500, p. 576-585.
0044-8486
10.1016/j.aquaculture.2018.10.045
2-s2.0-85055901775
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquaculture
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 576-585
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128948514586624

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