Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy

• Autor(a) principal: Fernandes, Dayanne C. [UNESP]
• Data de Publicação: 2019
• Outros Autores: Eto, Silas F. [UNESP], Moraes, Alessandra C. [UNESP], Prado, Ed Johnny R. [UNESP], Medeiros, Andrea S.R. [UNESP], Belo, Marco A.A. [UNESP], Samara, Samir I. [UNESP], Costa, Paulo I. [UNESP], Pizauro, João M. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.fsi.2018.12.074; http://hdl.handle.net/11449/188576
• topic: Chronic inflammation; Immunoglobulin Y; Phagocytosis; Teleost

The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti-A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti-Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection.