Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy

Bibliographic Details
Main Author: Fernandes, Dayanne C. [UNESP]
Publication Date: 2019
Other Authors: Eto, Silas F. [UNESP], Moraes, Alessandra C. [UNESP], Prado, Ed Johnny R. [UNESP], Medeiros, Andrea S.R. [UNESP], Belo, Marco A.A. [UNESP], Samara, Samir I. [UNESP], Costa, Paulo I. [UNESP], Pizauro, João M. [UNESP]
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://hdl.handle.net/11449/188576
http://dx.doi.org/10.1016/j.fsi.2018.12.074
Summary: The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti-A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti-Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection.
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spelling Fernandes, Dayanne C. [UNESP]Eto, Silas F. [UNESP]Moraes, Alessandra C. [UNESP]Prado, Ed Johnny R. [UNESP]Medeiros, Andrea S.R. [UNESP]Belo, Marco A.A. [UNESP]Samara, Samir I. [UNESP]Costa, Paulo I. [UNESP]Pizauro, João M. [UNESP]Universidade Estadual Paulista (Unesp)9819c2ce-4871-4118-bcc0-926d52189c21ac84c503-ae22-4cb8-9ab8-df83e049ad4b36e1bc75-ac9c-41bd-b758-475498370667b7679aa6-1eac-4cfc-a481-c8a09753d3ae56800480-380e-49cb-914c-65b834d0fe713abc9cf5-b68b-4aaa-bbe6-83a577a0d81bcb6f8d8f-d765-456a-bca6-d0583abbe60796e4ac04-7ce8-4309-b82c-db8bc8025155eb13f9cd-e712-4dac-a255-7e3d7461ebc12019-10-06T16:12:33Z2019-10-06T16:12:33Z2019-04-01Fish and Shellfish Immunology, v. 87, p. 51-61.1095-99471050-4648http://hdl.handle.net/11449/188576http://dx.doi.org/10.1016/j.fsi.2018.12.07410.1016/j.fsi.2018.12.0742-s2.0-85059552784The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti-A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti-Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection.Made available in DSpace on 2019-10-06T16:12:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Institute of Chemistry Sao Paulo State University (Unesp), AraraquaraDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), JaboticabalDepartment of Preventive Veterinary Medicine of Unesp School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), JaboticabalClinical Analysis Department School of Pharmaceutical Sciences São Paulo State University (Unesp), AraraquaraInstitute of Chemistry Sao Paulo State University (Unesp), AraraquaraDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), JaboticabalDepartment of Preventive Veterinary Medicine of Unesp School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), JaboticabalClinical Analysis Department School of Pharmaceutical Sciences São Paulo State University (Unesp), AraraquaraCNPq: 140505/2016-4CNPq: 429245/2016-551-61engFish and Shellfish Immunology-1-1-1-1-1-1-1-1-1Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPChronic inflammationImmunoglobulin YPhagocytosisTeleostPhagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess0000-0001-5845-3940[6]11449/1885762021-10-22 23:05:43.003oai:repositorio.unesp.br:11449/188576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T02:05:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.en.fl_str_mv Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
title Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
spellingShingle Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
Fernandes, Dayanne C. [UNESP]
Chronic inflammation
Immunoglobulin Y
Phagocytosis
Teleost
title_short Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
title_full Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
title_fullStr Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
title_full_unstemmed Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
title_sort Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy
author Fernandes, Dayanne C. [UNESP]
author_facet Fernandes, Dayanne C. [UNESP]
Eto, Silas F. [UNESP]
Moraes, Alessandra C. [UNESP]
Prado, Ed Johnny R. [UNESP]
Medeiros, Andrea S.R. [UNESP]
Belo, Marco A.A. [UNESP]
Samara, Samir I. [UNESP]
Costa, Paulo I. [UNESP]
Pizauro, João M. [UNESP]
author_role author
author2 Eto, Silas F. [UNESP]
Moraes, Alessandra C. [UNESP]
Prado, Ed Johnny R. [UNESP]
Medeiros, Andrea S.R. [UNESP]
Belo, Marco A.A. [UNESP]
Samara, Samir I. [UNESP]
Costa, Paulo I. [UNESP]
Pizauro, João M. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fernandes, Dayanne C. [UNESP]
Eto, Silas F. [UNESP]
Moraes, Alessandra C. [UNESP]
Prado, Ed Johnny R. [UNESP]
Medeiros, Andrea S.R. [UNESP]
Belo, Marco A.A. [UNESP]
Samara, Samir I. [UNESP]
Costa, Paulo I. [UNESP]
Pizauro, João M. [UNESP]
dc.contributor.authorID.fl_str_mv 9819c2ce-4871-4118-bcc0-926d52189c21
ac84c503-ae22-4cb8-9ab8-df83e049ad4b
36e1bc75-ac9c-41bd-b758-475498370667
b7679aa6-1eac-4cfc-a481-c8a09753d3ae
56800480-380e-49cb-914c-65b834d0fe71
3abc9cf5-b68b-4aaa-bbe6-83a577a0d81b
cb6f8d8f-d765-456a-bca6-d0583abbe607
96e4ac04-7ce8-4309-b82c-db8bc8025155
eb13f9cd-e712-4dac-a255-7e3d7461ebc1
dc.subject.por.fl_str_mv Chronic inflammation
Immunoglobulin Y
Phagocytosis
Teleost
topic Chronic inflammation
Immunoglobulin Y
Phagocytosis
Teleost
description The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti-A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti-Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-10-06T16:12:33Z
dc.date.available.fl_str_mv 2019-10-06T16:12:33Z
dc.date.issued.fl_str_mv 2019-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Fish and Shellfish Immunology, v. 87, p. 51-61.
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11449/188576
http://dx.doi.org/10.1016/j.fsi.2018.12.074
dc.identifier.issn.none.fl_str_mv 1095-9947
1050-4648
dc.identifier.doi.none.fl_str_mv 10.1016/j.fsi.2018.12.074
dc.identifier.scopus.none.fl_str_mv 2-s2.0-85059552784
identifier_str_mv Fish and Shellfish Immunology, v. 87, p. 51-61.
1095-9947
1050-4648
10.1016/j.fsi.2018.12.074
2-s2.0-85059552784
url http://hdl.handle.net/11449/188576
http://dx.doi.org/10.1016/j.fsi.2018.12.074
dc.language.iso.fl_str_mv eng
language eng
dc.relation.confidence.fl_str_mv -1
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dc.relation.ispartof.none.fl_str_mv Fish and Shellfish Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 51-61
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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