Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0074-02760170046 http://hdl.handle.net/11449/165858 |
Resumo: | BACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJECTIVE To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. METHODS Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. FINDINGS In the CF patients, an expansion of the peripheral blood TCD4(+) cells was observed during the treatment, and this persisted even after two years of antifungal treatment. In addition, these patients showed high serum levels of SP-D. CONCLUSION Our findings highlight the immunological changes CF patients undergo, during and after treatment, possibly due to the hypoxia triggered by pulmonary fibrosis and emphysema. |
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Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapyparacoccidioidomycosisT-lymphocyte subsetsantifungal agentspulmonary fibrosisemphysemasurfactant protein-DBACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJECTIVE To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. METHODS Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. FINDINGS In the CF patients, an expansion of the peripheral blood TCD4(+) cells was observed during the treatment, and this persisted even after two years of antifungal treatment. In addition, these patients showed high serum levels of SP-D. CONCLUSION Our findings highlight the immunological changes CF patients undergo, during and after treatment, possibly due to the hypoxia triggered by pulmonary fibrosis and emphysema.Univ Estadual Paulista, Fac Med, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias, Bauru, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias, Bauru, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilFundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Venturini, James [UNESP]Cavalcante, Ricardo Souza [UNESP]Sylvestre, Tatiane Fernanda [UNESP]Santos, Rodolfo Ferreira dos [UNESP]Moris, Daniela Vanessa [UNESP]Carvalho, Lidia Raquel [UNESP]Parreira de Arruda, Maria Sueli [UNESP]Golim, Marjorie de Assis [UNESP]Mendes, Rinaldo Poncio [UNESP]2018-11-29T01:20:52Z2018-11-29T01:20:52Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article748-755application/pdfhttp://dx.doi.org/10.1590/0074-02760170046Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 112, n. 11, p. 748-755, 2017.0074-0276http://hdl.handle.net/11449/16585810.1590/0074-02760170046S0074-02762017001100748WOS:000414023000003S0074-02762017001100748.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruz1,172info:eu-repo/semantics/openAccess2023-11-27T06:12:11Zoai:repositorio.unesp.br:11449/165858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:12:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
title |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
spellingShingle |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy Venturini, James [UNESP] paracoccidioidomycosis T-lymphocyte subsets antifungal agents pulmonary fibrosis emphysema surfactant protein-D |
title_short |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
title_full |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
title_fullStr |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
title_full_unstemmed |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
title_sort |
Increased peripheral blood TCD4(+) counts and serum SP-D levels in patients with chronic paracoccidioidomycosis, during and after antifungal therapy |
author |
Venturini, James [UNESP] |
author_facet |
Venturini, James [UNESP] Cavalcante, Ricardo Souza [UNESP] Sylvestre, Tatiane Fernanda [UNESP] Santos, Rodolfo Ferreira dos [UNESP] Moris, Daniela Vanessa [UNESP] Carvalho, Lidia Raquel [UNESP] Parreira de Arruda, Maria Sueli [UNESP] Golim, Marjorie de Assis [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author_role |
author |
author2 |
Cavalcante, Ricardo Souza [UNESP] Sylvestre, Tatiane Fernanda [UNESP] Santos, Rodolfo Ferreira dos [UNESP] Moris, Daniela Vanessa [UNESP] Carvalho, Lidia Raquel [UNESP] Parreira de Arruda, Maria Sueli [UNESP] Golim, Marjorie de Assis [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Venturini, James [UNESP] Cavalcante, Ricardo Souza [UNESP] Sylvestre, Tatiane Fernanda [UNESP] Santos, Rodolfo Ferreira dos [UNESP] Moris, Daniela Vanessa [UNESP] Carvalho, Lidia Raquel [UNESP] Parreira de Arruda, Maria Sueli [UNESP] Golim, Marjorie de Assis [UNESP] Mendes, Rinaldo Poncio [UNESP] |
dc.subject.por.fl_str_mv |
paracoccidioidomycosis T-lymphocyte subsets antifungal agents pulmonary fibrosis emphysema surfactant protein-D |
topic |
paracoccidioidomycosis T-lymphocyte subsets antifungal agents pulmonary fibrosis emphysema surfactant protein-D |
description |
BACKGROUND The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. OBJECTIVE To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. METHODS Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. FINDINGS In the CF patients, an expansion of the peripheral blood TCD4(+) cells was observed during the treatment, and this persisted even after two years of antifungal treatment. In addition, these patients showed high serum levels of SP-D. CONCLUSION Our findings highlight the immunological changes CF patients undergo, during and after treatment, possibly due to the hypoxia triggered by pulmonary fibrosis and emphysema. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-29T01:20:52Z 2018-11-29T01:20:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760170046 Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 112, n. 11, p. 748-755, 2017. 0074-0276 http://hdl.handle.net/11449/165858 10.1590/0074-02760170046 S0074-02762017001100748 WOS:000414023000003 S0074-02762017001100748.pdf |
url |
http://dx.doi.org/10.1590/0074-02760170046 http://hdl.handle.net/11449/165858 |
identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 112, n. 11, p. 748-755, 2017. 0074-0276 10.1590/0074-02760170046 S0074-02762017001100748 WOS:000414023000003 S0074-02762017001100748.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
748-755 application/pdf |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046680069668864 |