Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/96238 |
Resumo: | Herbal medicines have shown amazing results for the treatment of chronic diseases, especially the ones of the digestive tract (gastric and duodenal ulcers, ulcerative colitis and Crohn's disease), diabetes and cancer. The National Policy of Medicinal Plants and Herbal Medicines (Ministry of Health) proposes the study of several plant species, including plants containing the genus Astronium (Anacardiaceae) that includes species such as A. fraxinifolium, A. graveolens and A. urundeuva, which comprise anti-inflammatory, anti-ulcerogenic, healing and antimicrobial properties. In this context, the inclusion of technology in the improvement of medicinal plants should be encouraged since the strategy to incorporate plant extracts in nanostructured systems has optimized their properties. The antimicrobial activity of the extracts, which were incorporated or not into nanostructured systems, was evaluated by microdilution technique for the microorganisms Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Salmonella setubal (ATCC 19196), Helicobacter pylori (ATCC 43504) and Candida albicans (ATCC 18804). The plant extracts were tested at concentrations from 1000 to 7.81 μg/mL, and the following medications were used to check in order to see if they will work with each type of microorganisms: amoxicillin (100 μg/mL) for H. pylori; ampicillin (50 μg/mL) for the other bacteria, amphotericin B (32 μg/mL) and fluconazole (256 μg/mL) for C. albicans. After the incubation at 37 ºC/72 hours under microaerophilic condition for H. pylori; 37 ºC/24 hours for the other bacteria and 37 °C/48 hours for the yeast, spectrophotometric and visual readings were performed, with the developers 0.01% resazurin (bacteria) and 2% 2,3,5-triphenyltetrazolium chloride in order to determine the minimum inhibitory concentration (MIC). The incorporation was prepared into a nanostructured system comprising 10% oil phase (cholesterol), 10% surfactant ... |
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Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturadosAnacardiaceaAroeira do sertãoAgentes antiinfecciososMatéria médica vegetalMateria medica, VegetableHerbal medicines have shown amazing results for the treatment of chronic diseases, especially the ones of the digestive tract (gastric and duodenal ulcers, ulcerative colitis and Crohn's disease), diabetes and cancer. The National Policy of Medicinal Plants and Herbal Medicines (Ministry of Health) proposes the study of several plant species, including plants containing the genus Astronium (Anacardiaceae) that includes species such as A. fraxinifolium, A. graveolens and A. urundeuva, which comprise anti-inflammatory, anti-ulcerogenic, healing and antimicrobial properties. In this context, the inclusion of technology in the improvement of medicinal plants should be encouraged since the strategy to incorporate plant extracts in nanostructured systems has optimized their properties. The antimicrobial activity of the extracts, which were incorporated or not into nanostructured systems, was evaluated by microdilution technique for the microorganisms Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Salmonella setubal (ATCC 19196), Helicobacter pylori (ATCC 43504) and Candida albicans (ATCC 18804). The plant extracts were tested at concentrations from 1000 to 7.81 μg/mL, and the following medications were used to check in order to see if they will work with each type of microorganisms: amoxicillin (100 μg/mL) for H. pylori; ampicillin (50 μg/mL) for the other bacteria, amphotericin B (32 μg/mL) and fluconazole (256 μg/mL) for C. albicans. After the incubation at 37 ºC/72 hours under microaerophilic condition for H. pylori; 37 ºC/24 hours for the other bacteria and 37 °C/48 hours for the yeast, spectrophotometric and visual readings were performed, with the developers 0.01% resazurin (bacteria) and 2% 2,3,5-triphenyltetrazolium chloride in order to determine the minimum inhibitory concentration (MIC). The incorporation was prepared into a nanostructured system comprising 10% oil phase (cholesterol), 10% surfactant ...Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (Unesp)Bauab, Taís Maria [UNESP]Universidade Estadual Paulista (Unesp)Bonifácio, Bruna Vidal [UNESP]2014-06-11T19:28:03Z2014-06-11T19:28:03Z2014-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis140 f. : ils, tabs, figs.application/pdfBONIFÁCIO, Bruna Vidal. Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados. 2014. 140 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2014.http://hdl.handle.net/11449/96238000748196000748196.pdf33004030078P64910754838277580Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-24T18:42:33Zoai:repositorio.unesp.br:11449/96238Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:13.999528Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
title |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
spellingShingle |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados Bonifácio, Bruna Vidal [UNESP] Anacardiacea Aroeira do sertão Agentes antiinfecciosos Matéria médica vegetal Materia medica, Vegetable |
title_short |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
title_full |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
title_fullStr |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
title_full_unstemmed |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
title_sort |
Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados |
author |
Bonifácio, Bruna Vidal [UNESP] |
author_facet |
Bonifácio, Bruna Vidal [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bauab, Taís Maria [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bonifácio, Bruna Vidal [UNESP] |
dc.subject.por.fl_str_mv |
Anacardiacea Aroeira do sertão Agentes antiinfecciosos Matéria médica vegetal Materia medica, Vegetable |
topic |
Anacardiacea Aroeira do sertão Agentes antiinfecciosos Matéria médica vegetal Materia medica, Vegetable |
description |
Herbal medicines have shown amazing results for the treatment of chronic diseases, especially the ones of the digestive tract (gastric and duodenal ulcers, ulcerative colitis and Crohn's disease), diabetes and cancer. The National Policy of Medicinal Plants and Herbal Medicines (Ministry of Health) proposes the study of several plant species, including plants containing the genus Astronium (Anacardiaceae) that includes species such as A. fraxinifolium, A. graveolens and A. urundeuva, which comprise anti-inflammatory, anti-ulcerogenic, healing and antimicrobial properties. In this context, the inclusion of technology in the improvement of medicinal plants should be encouraged since the strategy to incorporate plant extracts in nanostructured systems has optimized their properties. The antimicrobial activity of the extracts, which were incorporated or not into nanostructured systems, was evaluated by microdilution technique for the microorganisms Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Salmonella setubal (ATCC 19196), Helicobacter pylori (ATCC 43504) and Candida albicans (ATCC 18804). The plant extracts were tested at concentrations from 1000 to 7.81 μg/mL, and the following medications were used to check in order to see if they will work with each type of microorganisms: amoxicillin (100 μg/mL) for H. pylori; ampicillin (50 μg/mL) for the other bacteria, amphotericin B (32 μg/mL) and fluconazole (256 μg/mL) for C. albicans. After the incubation at 37 ºC/72 hours under microaerophilic condition for H. pylori; 37 ºC/24 hours for the other bacteria and 37 °C/48 hours for the yeast, spectrophotometric and visual readings were performed, with the developers 0.01% resazurin (bacteria) and 2% 2,3,5-triphenyltetrazolium chloride in order to determine the minimum inhibitory concentration (MIC). The incorporation was prepared into a nanostructured system comprising 10% oil phase (cholesterol), 10% surfactant ... |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-11T19:28:03Z 2014-06-11T19:28:03Z 2014-02-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BONIFÁCIO, Bruna Vidal. Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados. 2014. 140 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2014. http://hdl.handle.net/11449/96238 000748196 000748196.pdf 33004030078P6 4910754838277580 |
identifier_str_mv |
BONIFÁCIO, Bruna Vidal. Atividade antimicrobiana in vitro de extratos hidroetanólicos de Astronium sp incorporados ou não em sistemas nanoestruturados. 2014. 140 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2014. 000748196 000748196.pdf 33004030078P6 4910754838277580 |
url |
http://hdl.handle.net/11449/96238 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
140 f. : ils, tabs, figs. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128745813311488 |