Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells

Detalhes bibliográficos
Autor(a) principal: Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]
Data de Publicação: 2018
Outros Autores: Gatto, Mariana [UNESP], Rodrigues, Daniela Ramos [UNESP], De Campos Soares, Ângela Maria Victoriano [UNESP], Calvi, Sueli Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1093/mmy/myx046
http://hdl.handle.net/11449/176223
Resumo: Cryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases.
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spelling Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cellsCryptococcus gattiiCryptococcus neoformansDNA damageNOPeripheral blood mononuclear cellsCryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Botucatu School of Medicine - UNESP Campus Botucatu Tropical Diseases Department, Av. Prof. Mario Rubens Guimaraes Montenegro, S/NBiosciences Institute - UNESP Campus Botucatu Microbiology and Immunology DepartmentBotucatu School of Medicine - UNESP Campus Botucatu Tropical Diseases Department, Av. Prof. Mario Rubens Guimaraes Montenegro, S/NBiosciences Institute - UNESP Campus Botucatu Microbiology and Immunology DepartmentUniversidade Estadual Paulista (Unesp)Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]Gatto, Mariana [UNESP]Rodrigues, Daniela Ramos [UNESP]De Campos Soares, Ângela Maria Victoriano [UNESP]Calvi, Sueli Aparecida [UNESP]2018-12-11T17:19:41Z2018-12-11T17:19:41Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article344-349application/pdfhttp://dx.doi.org/10.1093/mmy/myx046Medical Mycology, v. 56, n. 3, p. 344-349, 2018.1460-27091369-3786http://hdl.handle.net/11449/17622310.1093/mmy/myx0462-s2.0-850459565012-s2.0-85045956501.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycology0,9730,973info:eu-repo/semantics/openAccess2024-01-05T06:22:15Zoai:repositorio.unesp.br:11449/176223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:22:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
title Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
spellingShingle Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]
Cryptococcus gattii
Cryptococcus neoformans
DNA damage
NO
Peripheral blood mononuclear cells
title_short Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
title_full Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
title_fullStr Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
title_full_unstemmed Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
title_sort Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
author Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]
author_facet Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]
Gatto, Mariana [UNESP]
Rodrigues, Daniela Ramos [UNESP]
De Campos Soares, Ângela Maria Victoriano [UNESP]
Calvi, Sueli Aparecida [UNESP]
author_role author
author2 Gatto, Mariana [UNESP]
Rodrigues, Daniela Ramos [UNESP]
De Campos Soares, Ângela Maria Victoriano [UNESP]
Calvi, Sueli Aparecida [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]
Gatto, Mariana [UNESP]
Rodrigues, Daniela Ramos [UNESP]
De Campos Soares, Ângela Maria Victoriano [UNESP]
Calvi, Sueli Aparecida [UNESP]
dc.subject.por.fl_str_mv Cryptococcus gattii
Cryptococcus neoformans
DNA damage
NO
Peripheral blood mononuclear cells
topic Cryptococcus gattii
Cryptococcus neoformans
DNA damage
NO
Peripheral blood mononuclear cells
description Cryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:19:41Z
2018-12-11T17:19:41Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/mmy/myx046
Medical Mycology, v. 56, n. 3, p. 344-349, 2018.
1460-2709
1369-3786
http://hdl.handle.net/11449/176223
10.1093/mmy/myx046
2-s2.0-85045956501
2-s2.0-85045956501.pdf
url http://dx.doi.org/10.1093/mmy/myx046
http://hdl.handle.net/11449/176223
identifier_str_mv Medical Mycology, v. 56, n. 3, p. 344-349, 2018.
1460-2709
1369-3786
10.1093/mmy/myx046
2-s2.0-85045956501
2-s2.0-85045956501.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medical Mycology
0,973
0,973
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 344-349
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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