Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mmy/myx046 http://hdl.handle.net/11449/176223 |
Resumo: | Cryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases. |
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Repositório Institucional da UNESP |
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Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cellsCryptococcus gattiiCryptococcus neoformansDNA damageNOPeripheral blood mononuclear cellsCryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Botucatu School of Medicine - UNESP Campus Botucatu Tropical Diseases Department, Av. Prof. Mario Rubens Guimaraes Montenegro, S/NBiosciences Institute - UNESP Campus Botucatu Microbiology and Immunology DepartmentBotucatu School of Medicine - UNESP Campus Botucatu Tropical Diseases Department, Av. Prof. Mario Rubens Guimaraes Montenegro, S/NBiosciences Institute - UNESP Campus Botucatu Microbiology and Immunology DepartmentUniversidade Estadual Paulista (Unesp)Andrade, Jéssica Cristina Bilizario Noguerol [UNESP]Gatto, Mariana [UNESP]Rodrigues, Daniela Ramos [UNESP]De Campos Soares, Ângela Maria Victoriano [UNESP]Calvi, Sueli Aparecida [UNESP]2018-12-11T17:19:41Z2018-12-11T17:19:41Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article344-349application/pdfhttp://dx.doi.org/10.1093/mmy/myx046Medical Mycology, v. 56, n. 3, p. 344-349, 2018.1460-27091369-3786http://hdl.handle.net/11449/17622310.1093/mmy/myx0462-s2.0-850459565012-s2.0-85045956501.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycology0,9730,973info:eu-repo/semantics/openAccess2024-01-05T06:22:15Zoai:repositorio.unesp.br:11449/176223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:22:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
title |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
spellingShingle |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells Andrade, Jéssica Cristina Bilizario Noguerol [UNESP] Cryptococcus gattii Cryptococcus neoformans DNA damage NO Peripheral blood mononuclear cells |
title_short |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
title_full |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
title_fullStr |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
title_full_unstemmed |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
title_sort |
Cryptococcus neoformans and gattii promote DNA damage in human peripheral blood mononuclear cells |
author |
Andrade, Jéssica Cristina Bilizario Noguerol [UNESP] |
author_facet |
Andrade, Jéssica Cristina Bilizario Noguerol [UNESP] Gatto, Mariana [UNESP] Rodrigues, Daniela Ramos [UNESP] De Campos Soares, Ângela Maria Victoriano [UNESP] Calvi, Sueli Aparecida [UNESP] |
author_role |
author |
author2 |
Gatto, Mariana [UNESP] Rodrigues, Daniela Ramos [UNESP] De Campos Soares, Ângela Maria Victoriano [UNESP] Calvi, Sueli Aparecida [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Andrade, Jéssica Cristina Bilizario Noguerol [UNESP] Gatto, Mariana [UNESP] Rodrigues, Daniela Ramos [UNESP] De Campos Soares, Ângela Maria Victoriano [UNESP] Calvi, Sueli Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Cryptococcus gattii Cryptococcus neoformans DNA damage NO Peripheral blood mononuclear cells |
topic |
Cryptococcus gattii Cryptococcus neoformans DNA damage NO Peripheral blood mononuclear cells |
description |
Cryptococcosis, a systemic mycosis capable of disseminating to the central nervous system with frequent lethal effects, is caused by the species Cryptococus neoformans and Cryptococcus gattii. Several infectious agents such as virus, bacteria, and parasites may be associated to DNA damage and carcinogenesis in humans. Products of the oxidative metabolism, such as NO, produced as a host defense mechanism to destroy these pathogens, have been implicated in this damage process, due to excessive production related to an established chronic inflammatory response. Here, we investigated whether C. neoformans and/or C. gattii can cause DNA damage in human peripheral blood mononuclear cells (PBMCs) and whether this process is related to NO levels produced by PBMCs. We found that both species are equally able to induce genotoxicity in PBMCs. However, an association between DNA damage and high NO levels was only detected in relation to C. gattii. The results point to the possibility that patients with cryptococcosis are more susceptible to the development of other diseases. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:41Z 2018-12-11T17:19:41Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mmy/myx046 Medical Mycology, v. 56, n. 3, p. 344-349, 2018. 1460-2709 1369-3786 http://hdl.handle.net/11449/176223 10.1093/mmy/myx046 2-s2.0-85045956501 2-s2.0-85045956501.pdf |
url |
http://dx.doi.org/10.1093/mmy/myx046 http://hdl.handle.net/11449/176223 |
identifier_str_mv |
Medical Mycology, v. 56, n. 3, p. 344-349, 2018. 1460-2709 1369-3786 10.1093/mmy/myx046 2-s2.0-85045956501 2-s2.0-85045956501.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Mycology 0,973 0,973 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
344-349 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965520854777856 |