Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2017.12.009 http://hdl.handle.net/11449/170496 |
Resumo: | Diuron is one of the most used herbicide in the world, and its field application has been particularly increased in Brazil due to the expansion of sugarcane crops. Diuron has often been detected in freshwater ecosystems and it can be biodegraded into three main metabolites in the environment, the 3,4-dichloroaniline (DCA), 3,4-dichlorophenylurea (DCPU) and 3,4-dichlorophenyl-N-methylurea (DCPMU). Negative effects under aquatic biota are still not well established for diuron, especially when considering its presence in mixture with its different metabolites. In this study, we evaluated the effects of diuron alone or in combination with its metabolites, DCPMU, DCPU and 3,4-DCA on biochemical stress responses and biotransformation activity of the fish Oreochromis niloticus. Results showed that diuron and its metabolites caused significant but dispersed alterations in oxidative stress markers and biotransformation enzymes, except for ethoxyresorufin-O-deethylase (EROD) activity, that presented a dose-dependent increase after exposure to either diuron or its metabolites. Glutathione S-transferase (GST) activity was significant lower in gills after exposure to diuron metabolites, but not diuron. Diuron, DCPMU and DCA also decreased the multixenobiotic resistance (MXR) activity. Lipid peroxidation levels were increased in gill after exposure to all compounds, indicating that the original compound and diuron metabolites can induce oxidative stress in fish. The integration of all biochemical responses by the Integrated Biomarker Response (IBR) model indicated that all compounds caused significant alterations in O. niloticus, but DCPMU caused the higher alterations in both liver and gill. Our findings imply that diuron and its metabolites may impair the physiological response related to biotransformation and antioxidant activity in fish at field concentrations. Such alterations could interfere with the ability of aquatic animals to adapt to environments contaminated by agriculture. |
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Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus)BiotransformationFishHerbicideOxidative stressDiuron is one of the most used herbicide in the world, and its field application has been particularly increased in Brazil due to the expansion of sugarcane crops. Diuron has often been detected in freshwater ecosystems and it can be biodegraded into three main metabolites in the environment, the 3,4-dichloroaniline (DCA), 3,4-dichlorophenylurea (DCPU) and 3,4-dichlorophenyl-N-methylurea (DCPMU). Negative effects under aquatic biota are still not well established for diuron, especially when considering its presence in mixture with its different metabolites. In this study, we evaluated the effects of diuron alone or in combination with its metabolites, DCPMU, DCPU and 3,4-DCA on biochemical stress responses and biotransformation activity of the fish Oreochromis niloticus. Results showed that diuron and its metabolites caused significant but dispersed alterations in oxidative stress markers and biotransformation enzymes, except for ethoxyresorufin-O-deethylase (EROD) activity, that presented a dose-dependent increase after exposure to either diuron or its metabolites. Glutathione S-transferase (GST) activity was significant lower in gills after exposure to diuron metabolites, but not diuron. Diuron, DCPMU and DCA also decreased the multixenobiotic resistance (MXR) activity. Lipid peroxidation levels were increased in gill after exposure to all compounds, indicating that the original compound and diuron metabolites can induce oxidative stress in fish. The integration of all biochemical responses by the Integrated Biomarker Response (IBR) model indicated that all compounds caused significant alterations in O. niloticus, but DCPMU caused the higher alterations in both liver and gill. Our findings imply that diuron and its metabolites may impair the physiological response related to biotransformation and antioxidant activity in fish at field concentrations. Such alterations could interfere with the ability of aquatic animals to adapt to environments contaminated by agriculture.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP – Sao Paulo State University Department of Chemistry and Environmental ScienceUEG – Goias State University University Unit of Exact and Technological Science (UnUCET)Department of Environmental Sciences University of CaliforniaFURB – Fundação Universidade Regional de Blumenau Department of Natural SciencesUNESP – Sao Paulo State University Department of Chemistry and Environmental ScienceFAPESP: 2011/52061-8FAPESP: 2013/16948-3FAPESP: 2014/18825-9CNPq: 302158/2015CNPq: 307603/2014-8CNPq: 473245/2009-4Universidade Estadual Paulista (Unesp)University Unit of Exact and Technological Science (UnUCET)University of CaliforniaFURB – Fundação Universidade Regional de BlumenauFelício, Andréia Arantes [UNESP]Freitas, Juliane Silberschmidt [UNESP]Scarin, Jéssica Bolpeti [UNESP]de Souza Ondei, LucianaTeresa, Fabrício BarretoSchlenk, Danielde Almeida, Eduardo Alves2018-12-11T16:51:03Z2018-12-11T16:51:03Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article248-256application/pdfhttp://dx.doi.org/10.1016/j.ecoenv.2017.12.009Ecotoxicology and Environmental Safety, v. 149, p. 248-256.1090-24140147-6513http://hdl.handle.net/11449/17049610.1016/j.ecoenv.2017.12.0092-s2.0-850388526372-s2.0-85038852637.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safety1,2011,201info:eu-repo/semantics/openAccess2024-01-12T06:28:41Zoai:repositorio.unesp.br:11449/170496Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-12T06:28:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
title |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
spellingShingle |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) Felício, Andréia Arantes [UNESP] Biotransformation Fish Herbicide Oxidative stress |
title_short |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
title_full |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
title_fullStr |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
title_sort |
Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus) |
author |
Felício, Andréia Arantes [UNESP] |
author_facet |
Felício, Andréia Arantes [UNESP] Freitas, Juliane Silberschmidt [UNESP] Scarin, Jéssica Bolpeti [UNESP] de Souza Ondei, Luciana Teresa, Fabrício Barreto Schlenk, Daniel de Almeida, Eduardo Alves |
author_role |
author |
author2 |
Freitas, Juliane Silberschmidt [UNESP] Scarin, Jéssica Bolpeti [UNESP] de Souza Ondei, Luciana Teresa, Fabrício Barreto Schlenk, Daniel de Almeida, Eduardo Alves |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University Unit of Exact and Technological Science (UnUCET) University of California FURB – Fundação Universidade Regional de Blumenau |
dc.contributor.author.fl_str_mv |
Felício, Andréia Arantes [UNESP] Freitas, Juliane Silberschmidt [UNESP] Scarin, Jéssica Bolpeti [UNESP] de Souza Ondei, Luciana Teresa, Fabrício Barreto Schlenk, Daniel de Almeida, Eduardo Alves |
dc.subject.por.fl_str_mv |
Biotransformation Fish Herbicide Oxidative stress |
topic |
Biotransformation Fish Herbicide Oxidative stress |
description |
Diuron is one of the most used herbicide in the world, and its field application has been particularly increased in Brazil due to the expansion of sugarcane crops. Diuron has often been detected in freshwater ecosystems and it can be biodegraded into three main metabolites in the environment, the 3,4-dichloroaniline (DCA), 3,4-dichlorophenylurea (DCPU) and 3,4-dichlorophenyl-N-methylurea (DCPMU). Negative effects under aquatic biota are still not well established for diuron, especially when considering its presence in mixture with its different metabolites. In this study, we evaluated the effects of diuron alone or in combination with its metabolites, DCPMU, DCPU and 3,4-DCA on biochemical stress responses and biotransformation activity of the fish Oreochromis niloticus. Results showed that diuron and its metabolites caused significant but dispersed alterations in oxidative stress markers and biotransformation enzymes, except for ethoxyresorufin-O-deethylase (EROD) activity, that presented a dose-dependent increase after exposure to either diuron or its metabolites. Glutathione S-transferase (GST) activity was significant lower in gills after exposure to diuron metabolites, but not diuron. Diuron, DCPMU and DCA also decreased the multixenobiotic resistance (MXR) activity. Lipid peroxidation levels were increased in gill after exposure to all compounds, indicating that the original compound and diuron metabolites can induce oxidative stress in fish. The integration of all biochemical responses by the Integrated Biomarker Response (IBR) model indicated that all compounds caused significant alterations in O. niloticus, but DCPMU caused the higher alterations in both liver and gill. Our findings imply that diuron and its metabolites may impair the physiological response related to biotransformation and antioxidant activity in fish at field concentrations. Such alterations could interfere with the ability of aquatic animals to adapt to environments contaminated by agriculture. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:51:03Z 2018-12-11T16:51:03Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2017.12.009 Ecotoxicology and Environmental Safety, v. 149, p. 248-256. 1090-2414 0147-6513 http://hdl.handle.net/11449/170496 10.1016/j.ecoenv.2017.12.009 2-s2.0-85038852637 2-s2.0-85038852637.pdf |
url |
http://dx.doi.org/10.1016/j.ecoenv.2017.12.009 http://hdl.handle.net/11449/170496 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 149, p. 248-256. 1090-2414 0147-6513 10.1016/j.ecoenv.2017.12.009 2-s2.0-85038852637 2-s2.0-85038852637.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety 1,201 1,201 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
248-256 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803047330034745344 |